Stress associated proteins （SAPs） play an important role in plant response to abiotic stress，but there was little information about the SAP family and function of its member in wheat. In this study，62 SAP genes were identified from wheat，and analyses of these members were conducted combined phylogenetic tree， gene structure and constitutive motifs. The expression pattern of TaSAP1；1，a member belongs to SAP family and responding to low-Pi stress in our previous study was investigated by qRT-PCR，and the results showed its expression increased after both stresses. Biodiversity analysis revealed that there was diversity of TaSAP1；1 gene and subcellular localization analysis showed that TaSAP1；1 targeted onto nucleus. The expression vector was constructed and transformed into tobacco to obtain the overexpression lines （OE）. Combined phenotypic analysis and parameters comparison such as fresh weight，leaf area and chlorophyll content in plants of OE lines and wild-type （WT），it was showed that overexpression of TaSAP1；1 in tobacco not only enhanced the tolerance of seedlings to salt stress，but also to low-Pi stress. The improved tolerance may be related to the increased stomatal closure rate resulting from up-regulation of some ABA signal pathway related genes （NtSAPKs），and the increased expression of some phosphate transporters （PTS） genes，respectively. In addition，the interaction between TaSAP1；1 and a transaminase named TaAMTR detected by yeast-two hybridization assay may also be involved in the process of stress resistance. The results not only enrich the understanding of wheat SAP family， but also have a new insight on the function of its members involved in abiotic stress，which provides a theoretical basis for the genetic improvement of crop comprehensive stress resistance.