HIPPs (heavy metal-associated isoprenylated plant proteins) are key proteins for the safety transportation of metallic ions in the plants cell, which plays an important role in the maintenance of metal ion homeostasis and detoxification mechanism. To date, most studies on HIPPs gene family focus on model plants Oryza sativa L. and Arabidopsis thaliana L., while relatively few reports have been reported in Brassica napus L.. In this study, In this study, 104 HIPPs genes were identified in the genome of Brassica napus L. (Darmor-bzh v10) using Bioinformatics methods, which were unevenly distributed on 19 chromosomes of B. napus L.. By analyzing the physicochemical properties, we found that the amino acid number, protein molecular weight and protein hydrophobicity index of BnaHIPPs genes ranged from 120 to 630 aa, 13 827.56 to 64 467.05 Da, and -1.146 to -0.17, respectively. And the average isoelectric point was 8.07. Their expression patterns have certain spatiotemporal expression properties in different tissues and developmental periods, with the highest expression in roots. The expression pattern of BnaHIPPs were altered in response to Cd2+ stress treatment, and the relative expression levels of BnaA08p24150 and BnaA09p41730 were up-regulated in both roots and leaves. The phylogenetic relationship confirmed that 104 BnaHIPPs gene were divided into five distinct clades. In addition, collinearity analysis elucidated that during the evolution of BnaHIPPs gene famliy, most BnaHIPPs participate in the expansion of the BnaHIPPs gene family in the form of fragment replication, and only BnaC05p3800, BnaC05p3810, BnaC05p3820 and BnaC05p3830 were found to involve in the replication events of BnaHIPPs in the form of tandem repetition. Our results will lay the foundation for further research on the BnaHIPPs gene family in B. napus L..