2025-4-4- 21
Home > Archive>Volume 23, Issue 3, 2022 >811-822. DOI:10.13430/j.cnki.jpgr.20211203002
PDF HTML XML Export Cite reminder
Genetic Effect of Sterile Spikelet Number-related QTL-qSsnps-5D and Its Use in Wheat Varieties
Author:
Affiliation:

1.School of Agriculture,Ludong University/Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong;2.Modern Breeding Technology Center,Liaocheng Academy of Agricultural Sciences,Shandong Liaocheng;3.Shandong Agricultural Technology Extension Center

Fund Project:

Shandong Provincial Key Research and Development Program（2019GNC106126），National Natural Science Foundation of China（31871612），Youth Innovation Science and Technology Program of Colleges and Universities in Shandong Province （2019KJF002），Yantai Science and Technology Planning Project（2020XCZX045）

  • Article
    • | |
  • Metrics
    • |
  • Reference [39]
    • | | | |
  • Comments
    Abstract:

    qSsnps-5D，derived from the backbone parental line Jing 411（J411），is a major stable QTL for sterile spikelet number per spike（SSNPS）. This study aimed to decipher the genetic effect of this QTL and its use in wheat varieties. Two populations，including a recombinant inbred lines derived from the cross of Kenong 9204（KN9204）and Jing 411（KJ-RIL），and a natural mapping population comprised by 314 wheat authorized varieties or advanced lines，were involved. The experiment results showed that qSsnps-5D was confirmed in an interval of 3.41 Mb from 0.72 Mb to 4.13 Mb on chromosome 5D among 8 datasets. Excellent allele of qSsnps5D from J411 could increase spikes per plant but significantly decrease thousand kernel weight in both KJ-RIL and the natural mapping populations. However，the genetic effects of qSsnps-5D on kernel number per spike and yield per plant were differing between the two populations. Two close linkage markers of qSsnps-5D，i.e.，AX110565536 and AX-86170796 were used to specify the breeding utilization characteristics of qSsnps-5D in the natural mapping population. The excellent haplotype for qSsnps-5D（Hap-GG-CC）was often found（80.60%） in the foreign varieties. Among the Chinese varieties，higher rates of excellent haplotype（Hap-GG-CC）were found from varieties of Qinghai province，Sichuan province and Henan province，but the rates of haplotype HapGG-CC were lower in varieties of Shandong，Beijing，Shaanxi and Hebei. The rates of Hap-GG-CC in varieties released from 1980 s to present were constantly declined. To facilitate the application of qSsnps-5D in future molecular breeding programs，we developed a PCR-based InDel molecular marker named as 5D-1620921. Altogether，this study provided insights for the application of qSsnps-5D in molecular breeding programs of wheat.

    Reference
    [1]刘阳娜，刘丽华，张风廷，张立平，苑少华，张明明，李宏博，庞斌双，赵昌平. 利用永久F2群体定位小麦穗部性状相关的QTL. 麦类作物学报, 2021, 41(05):525-531.
    Liu Y N, Liu L H, Zhang FT, Zhang L P, Yuan S H, Zhang MM, Li H B, Pang B S, Zhao C P. QTL mapping for spike traits of wheat using immortalized F2 population. Journal of Triticeae Crops, 2021, 41(05):525-531.
    [2] Sreenivasulu N, Schnurbusch T. A genetic playground for enhancing grain number in cereals. Trends Plant Science, 2012, 17(2):91-101.
    [3] Gupta P K, Mir R R, Mohan A, Kumar J. Wheat genomics: present status and future prospects. International journal of plant genomics, 2008, 2008 : 896451.
    [4]周阳，何中虎，陈新民，王德森，张勇，张改生. 30余年来北部冬麦区小麦品种产量改良遗传进展.作物学报，2007，(09):1530-1535.
    Zhou Y, He Z H, Chen X M, Wang D S, Zhang Y, Zhang G S. Genetic gain of wheat breeding for yield in northern winter wheat zone over 30 years. Acta Agronomica Sinica, 2007, (09):1530-1535.
    [5]刘凯，邓志英，李青芳，张莹，孙彩铃，田纪春，陈建省. 利用高密度SNP遗传图谱定位小麦穗部性状基因.作物学报，2016，42(06):820-831.
    Liu K, Deng Z Y, Li Q F, Zhang Y, Sun CL, Tian J C, Chen J S. Mapping QTLs for wheat panicle traits with high density SNP genetic map. Acta Agronomica Sinica, 2016,42(06):820-831.
    [6] Brancourt-Hulmel M, Doussinault G, Lecomte C, Bérard P, Le Buanec B, Trottet M. Genetic improvement of agronomic traits of winter wheat cultivars released in France from 1946 to 1992. Crop Science, 2003, 43:37-45.
    [7]李维平.高产小麦育种如何取得突破.科技导报，1998，(04):45-47.
    Li W P. Strategy for higher yield of China’s wheat breeding. Science & Technology Review, 1998, (04):45-47.
    [8]胡文静，李东升，裔新，张春梅，张勇. 小麦穗部性状和株高的QTL定位及育种标记开发和验证.作物学报,2021,1-12.
    Hu WJ, Li D S, Yi X, Zhang C M, Zhang Y. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat. Acta Agronomica Sinica, 2021, 1-12.
    [9] Chen Z, Cheng X, Chai L, Wang Z, Du D, Wang Z, Bian R, Zhao A, Xin M, Guo W, Hu Z, Peng H, Yao Y, Sun Q, Ni Z. Pleiotropic QTL influencing spikelet number and heading date in common wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 2020,133(6):1825-1838.
    [10]王佳佳.小麦基部小穗不育突变体穗部性状的QTL定位.山东：山东农业大学, 2014.
    Wang JJ. QTL Mapping for spike related traits using a mutant line of basal spikelets sterile in wheat. Shandong: Shandong Agricultural University,2014.
    [11] Cui F, Ding A M, Li J, Zhao C H, Wang L, Wang X Q, Qi X L, Li X F, Li G Y, Gao J R, Wang H G. QTL detection of seven spike-related traits and their genetic correlations in wheat using two related RIL populations. Euphytica, 2012, 186: 177–192.
    [12] Cui F, Zhao C H, Ding A M, Li J, Wang L, Li X F, Bao Y G, Li J M, Wang H G. Construction of an integrative linkage map and QTL mapping of grain yield-related traits using three related wheat RIL populations. Theoretical and Applied Genetics, 2014, 127(3):659-75.
    [13] Huang X Q, Kempf H, Canal M W, Roder M S. Advanced back-cross QTL analysis in progenies derived from a cross between a German elite winter wheat variety and a synthetic wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 2004, 109: 933–943.
    [14] Ma Z Q, Zhao D M, Zhang C Q, Zhang Z Z, Xue S L, Lin F,Kong Z X, Tian D G, Luo Q Y. Molecular genetic analysis of five spike-related traits in wheat using RIL and immortalized F2 populations. Molecular Genetics and Genomics, 2007, 277: 31–42.
    [15] Deng S, Wu X, Wu Y, Zhou R, Wang H, Jia J, Liu S. Characterization and precise mapping of a QTL increasing spike number with pleiotropic effects in wheat. Theoretical and Applied Genetics, 2011, 122:281–289.
    [16] Li T, Deng G B, Su Y, Yang Z, Tang Y Y, Wang J H, Qiu X B, Pu X, Li J, Liu Z H, Zhang H L, Liang J J, Yang W Y, Yu M Q, Wei Y M, Long H. Identification and validation of two major QTLs for spike compactness and length in bread wheat (Triticum aestivum L.) showing pleiotropic effects on yield-related traits. Theoretical and Applied Genetics, 2021, 134(11):3625-3641.
    [17] Xu H W, Zhang R Q, Wang M M, Li L H, Yan L, Wang Z, Zhu J, Chen X Y, Zhao A J, Su Z Q, Xing J W, Sun Q X, Ni Z F. Identification and characterization of QTL for spike morphological traits, plant height and heading date derived from the D genome of natural and resynthetic allohexaploid wheat. Theoretical and Applied Genetics, 2021, doi: 10.1007/s00122-021-03971-3. Online ahead of print.
    [18] Yang Y, Amo A, Wei D, Chai Y M, Zheng J, Qiao P F, Cui C, Lu S, Chen L, Hu Y G. Large-scale integration of meta-QTL and genome-wide association study discovers the genomic regions and candidate genes for yield and yield-related traits in bread wheat. Theoretical and Applied Genetics, 2021, 134(9):3083-3109.
    [19]武炳瑾，简俊涛，张德强，马文洁，冯洁，崔紫霞，张传量，孙道杰. 利用90k芯片技术进行小麦穗部性状QTL定位.作物学报，2017，43(07):1087-1095.
    Wu BJ, Jian J T, Zhang D Q, Ma W J, Feng J, Cui Z X, Zhang C L, Sun D J. QTL mapping for spike traits of wheat using 90K chip technology. Acta Agronomica Sinica, 2017,43(07):1087-1095.
    [20]肖永贵，路亚明，闻伟锷，陈新民，夏先春，王德森，李思敏，童依平，何中虎. 小麦骨干亲本京411及衍生品种苗期根部性状的遗传.中国农业科学，2014，47(15):2916-2926.
    Xiao Y G, Lu Y M, Wen W E, Chen X M, Xia X C, Wang D S, Li S M, Tonf Y P, He Z H. Genetic contribution of seedling root traits among elite wheat parent Jing 411 to its derivatives. Scientia Agricultura Sinica, 2014,47(15):2916-2926.
    [21] Cui F, Zhang N, Fan X L, Zhang W, Zhao C H, Yang L J, Pan R Q, Chen M, Han J, Zhao X Q, Ji J, Tong Y P, Zhang H X, Jia J Z, Zhao G Y, Li J M. Utilization of a Wheat660K SNP array-derived high-density genetic map for high-resolution mapping of a major QTL for kernel number. Scientific Reports, 2017, 7(1):3788.
    [22] Cui F, Fan X L, Chen M, Zhang N, Zhao C H, Zhang W, Han J, Ji J, Zhao X Q, Yang L J, Zhao Z W, Tong Y P, Wang T, Li J M. QTL detection for wheat kernel size and quality and the responses of these traits to low nitrogen stress. Theoretical and Applied Genetics, 2016, 129(3):469-84.
    [23]赵春华，樊小莉，王维莲，张玮，韩洁，陈梅，纪军，崔法，李俊明. 小麦候选骨干亲本科农9204遗传构成及其传递率.作物学报，2015，41(04):574-584.
    Zhao C H, Fan X L, Wang W L, Zhang W, Han H, Chen M, Ji J, Cui F, Li J M. Genetic composition and its transmissibility analysis and wheat candidate backbone parent Kenong 9204. Acta Agronomica Sinica, 2015,41(04):574-584.
    [24]刘朦朦，张萌娜，张倩倩，刘锡建，郭宇航，孙靳惠，武亚瑞，王素容，吴永振，孙晗，崔法，赵春华. 小麦旗叶宽主效QTL QFlw-5B遗传效应解析. 麦类作物学报，2019，39(12):1399-1405.
    Liu M M, Zhang M N, Zhang Q Q, Liu X J, Guo Y H, Sun Q H, Wu Y R, Wang S R, Wu Y Z, Sun H, Cui F, Zhao C H. Genetic analysis of a major stable QTL QFlw-5B for wheat flag leaf width. Journal of Triticeae Crops, 2019, 39(12):1399-1405.
    [25]崔俊鹏，赵慧，张倩倩，宫娜，刘朦朦，张萌娜，侯玉竹，刘成，李林志，周芳婷，吴永振，孙晗，赵春华，崔法.小麦穗粒数主效QTL-qKnps-4A遗传效应解析.分子植物育种,2019,17(11):3632-3640.
    Cui J P, Zhao H, Zhang Q Q, Gong N, Liu M M, Zhang M N, Hou YZ, Liu C, Li L Z, Zhou F T, Wu Y Z, Sun H, Zhao C H, Cui F. Genetic effects analysis of major QTL-qKnps-4A for kernel per spike in common wheat. Molecular Plant Breeding, 2019, 17(11):3632-3640.
    [26]张倩倩，闫学梅，刘锡建，张萌娜，刘朦朦，周芳婷，吴永振，孙晗，赵春华，崔法.小麦穗粒数主效QTL-qKnps-2A遗传效应解析.分子植物育种,2020,18(15):5003-5009.
    Zhang Q Q, Yan XM, Liu X J, Zhang M N, Liu M M, Zhou F T, Wu Y Z, Sun H, Zhao C H, Cui F. Genetic analysis of qKnps-2A: a major stable QTL for kernel number per spike in common wheat. Molecular Plant Breeding, 2020,18(15):5003-5009.
    [27] Stacey J, Isaac P G . Isolation of DNA from plants. Methods in Molecular Biology, 1994, 28:9.
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

Copy
Share
Article Metrics
  • Abstract:424
  • PDF: 1107
  • HTML: 0
  • Cited by: 0
History
  • Received:December 03,2021
  • Revised:December 13,2021
  • Adopted:December 21,2021
  • Online: May 10,2022
Article QR Code
You are the 604630th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.