NAC transcription factors are known to be involved into responses to biotic and abiotic stresses in plants. In this study，we cloned by sequence homology 3 SsNAC genes from sugarcane wild species S. spontaneum L.，named as SsNAC2，SsNAC3 and SsNAC4. Bioinformatic analysis showed that the full-length coding sequences of SsNAC2，SsNAC3 and SsNAC4 were 931 bp，486 bp，and 781 bp，encoding 309，162 and 259 amino acids，respectively. The subcellular location prediction indicated that the deduced proteins of SsNAC2， SsNAC3 and SsNAC4 were localized in the nucleus. The promoters of SsNAC2，SsNAC3 and SsNAC4 were predicted with putative stress-responsive cis-acting elements including an ABRE，LTR，MBS，MYB and STRE. Phylogenetic tree analysis showed that SsNAC2 within the OsNAC3 subgroup was closely related to Sorghum SbNAC68 gene. SsNAC4 and its homolog gene sorghum SbNAC82 belonged to NAC2 subgroup，and SsNAC3 in the ATAF subgroup was closely related to Miscanthus MlNAC1. The tissue-specific analysis showed that SsNAC2 and SsNAC3 were highly expressed in stem and leaves. The transcription profile analysis showed that SsNAC2， SsNAC3 and SsNAC4 were variable on expression under different stress conditions. It′s speculated that SsNAC2， SsNAC3 and SsNAC4 might be involved in regulation of abiotic and biotic stresses including drought，low temperature，salinity，pathogenic fungi，etc. The transcripts of SsNAC2，SsNAC3 andSsNAC4 were inducible by ABA and MeJA treatments. These results provided theoretical basis for future deciphering the functions of NAC genes under stresses conditions in S. Spontaneum L.