Long non-coding RNAs（lncRNAs）are functional RNA molecules with>200 nucleotides in length，and play an important regulatory role in the growth and development of plant. We previously identified a salt stress-related lncRNA in soybean，called lncRNA77580. Deletion and overexpression of lncRNA77580 were found to alter the expression of several neighboring protein-coding genes. In this study，we explored the function and regulation network of lncRNA77580 by the transcriptome analysis of lncRNA77580 transgenic hairy root. Over-expressing lncRNA77580 in soybean hairy roots showed salt sensitive. We generated the edited lines harboring>4kb fragment deletion in lncRNA77580 using dual-sgRNA/Cas9 technology（mutation efficiency：12.5%）. By conducting the transcriptome sequencing on the hairy roots with lncRNA77580 overexpressing or knockout，significant changes on transcriptional profiles of the transgenic hairy roots were detected. GO enrichment and KEGG pathway analysis of differentially expressed genes（DEGs）showed that overexpressing or knockout of lncRNA77580 had modified the transcriptional profiles in a large number of transcription factors and signaling genes，which are annotated to be related with plant stress response and plant growth and development. Collectively，these results suggested that lncRNA77580 is a long non-coding RNA with important functions in stress response of soybean and plant growth and development.