In this study，we cloned the transcription factor gene FtMYB41 in tartary buckwheat cultivar Pinku-1. Sequence analysis showed that the MYB super gene family member FtMYB41 contained one 5′UTR and two introns，with the complete coding sequence of 705bp which encodes for 234 amino acids. Phylogenetic tree analysis showed that FtMYB41 protein had close homology with tomato NP_001234262.1 and tobacco NP_001312384.1. Transcriptional activation analysis revealed the transcriptional activation activity at FtMYB41 C-terminal. Moreover，the FtMYB41 gene had the higher expression in roots and was induced by drought and salt stresses. The expression level of FtMYB41 gene was significantly increased under drought stress for 9 h. Overexpressing FtMYB41 gene in Arabidopsis thaliana（L.）Heynh. and tartary buckwheat hair root resulted in improved drought resistance by increasing germination rate，increasing CAT and SOD activity，and decreasing MDA content.