Field weeds are the main factors affecting crop quality and yield，and chemical weed control is the primary strategy in modern agricultural production. Acetolactate synthase （ALS），also known as acetohydroxyacid synthase，is the critical enzyme in the biosynthesis of branched amino acids in plants. ALS inhibitor herbicides are also called ALS herbicides，which inhibit the biosynthesis of branched-chain amino acids in plants by disturbing the binding of ALS to substrates，achieving the purpose of killing weeds. With the widespread application of ALS herbicides in agricultural production，the problem of herbicide residues on subsequent crops is becoming more and more serious. The impact on crop yield and quality is particularly obvious. Thus，breeding for cultivars resistant to these herbicides would bring great advantage in effective weed control. Germplasm resistant to ALS herbicides has been created in a variety of crops through chemical mutagenesis and natural mutation，and resistant varieties have been successfully developed. In this study，the characteristics，types and scope of application for ALS herbicides，the resistance mechanism of ALS herbicide-resistant crops，and the research progress of germplasm creation and utilization of ALS herbicide-resistant crops were reviewed，which provides a better understanding of crops resistant to ALS herbicides. The innovation of herbicide germplasm and variety selection can provide reference，and make a simple prediction for the possible development of ALSresistant herbicide crops in the future.