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Home > Archive>Volume 23, Issue 5, 2022 >1438-1445. DOI:10.13430/j.cnki.jpgr.20220128003
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Allelic Variation of The TaGS2 Gene is Associated with Kernel Weight in Chinese Bread Wheat
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Affiliation:

1.Wheat Research Institute, Henan Academy of Agricultural Science/Henan Province Key Laboratory of Wheat Biology;2.Agronomy College, National Key Laboratory of Wheat and Corn Crop Science / Collaborative Innovation Center of Henan Grain Crops/Agronomy College, Henan Agricultural University;3.Puyang city soil fertilizer station, Henan Puyang

Fund Project:

Henan Province Science Foundation for Youths (202300410527)

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    Abstract:

    Kernel weight is one of the three elements contributing to the wheat yield, and kernel size is highly correlated with kernel weight. Therefore, the study of kernel size has a great significance to improve the wheat yield. The TaGS2 gene positively regulates kernel size and kernel weight in wheat. In order to further explore allelic variation of TaGS2 gene in wheat, specific primers were designed to amplify the genomic DNA sequence of three TaGS2 homoeologues and identify the polymorphisms in 12 samples of 268 wheat cultivars from the Yellow and Huai wheat regions. A deletion of three nucleotides (CTT) at position 3,002 bp in the fourth exon of TaGS2-2B genomic DNA sequence was detected in some cultivars, resulting in a deletion of one phenylalanine (F) at the 306 position. Both alleles without and with the 3 bp deletion was designated as TaGS2-2B-a and TaGS2-2B-b, respectively. In wheat cultivars carrying TaGS2-2B-a allele, thousand-kernel weight, kernel length, kernel width, kernel area, and kernel circumference were significantly higher than those of wheat cultivars harboring TaGS2-2B-b allele. Collectively, this study provided a relatively superior allele (TaGS2-2B-a) with positive effects in increasing kernel weight in wheat cultivars.

    Reference
    [1] 张弛. 中国未来人口发展的粮食安全与耕地保障. 今日财富, 2021(11): 225-226.Zhang C. TheSChineseSfoodSsecuritySandSarableSlandSforSpopulationSdevelopmentSinStheSfuture. Fortune Today, 2021, (11): 225-226. (in Chinese)
    [2] 刘志勇, 王道文, 张爱民, 梁翰文, 吕慧颖, 邓向东, 葛毅强, 魏 珣, 杨维才. 小麦育种行业创新现状与发展趋势. 植物遗传资源学报, 2018, 19(3): 430-434.Liu Z Y, Wang D W, Zhang A M, Liang H W, Lv H Y, Deng X D, Ge Y Q, Wei X, Yang W C. Current status and perspective of wheat genomics, genetics, and breeding. Journal of Plant Genetic Resources, 2018, 19(3): 430-434. (in Chinese)
    [3] Cui F, Ding A M, Li J, Zhao C H, Li X F, Feng D S, Wang X Q, Wang L, Gao J R, Wang H G. Wheat kernel dimensions: how do they contribute to kernel weight at an individual QTL level. Journal of Genetics, 2011, 90(3): 409-425.
    [4] 方良学. 从小麦产量因素的相对重要性谈小麦增产技术途径. 河南农林科技, 1982, (10): 7?9.Fang L X. The technique of increasing wheat yield from the relative importance of wheat yield elements. Henan Agriculture and Forestry Technology, 1982,(10): 7-9. (in Chinese)
    [5] 张煜. 河南省小麦品种产量的遗传改进及相关生物学特性的研究. 河南农业大学博士论文, 河南郑州, 2015.Zhang Y. Genetic Improvement in Grain Yield and Associated Biological Characteristics of Wheat in Henan province of China. PhD Dissertation of Henan Agricultural University, Zhengzhou, Henan, China, 2015. (in Chinese)
    [6] Brocklehurst P. Factors controlling grain weight in wheat. Nature, 1977, 24(5600): 348-349.
    [7] Su Z Q, Hao C Y, Wang L F, Dong Y C, Zhang X Y. Identification and development of a functional marker of TaGW2 associated with grain weight in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 2011, 122(1): 211-223.
    [8] Wang W , Simmonds J, Pan Q L, Davidson D, He F, Battal A, Akhunova A, Trick H N, Uauy C, Akhunov E. Gene editing and mutagenesis reveal inter-cultivar differences and additivity in the contribution of TaGW2 homoeologues to grain size and weight in wheat. Theoretical and Applied Genetics, 2018, 131: 2463-2475
    [9] Yi Z , Da L , Zhang D B, Zhao X G, Cao X M, Dong L L, Liu J X, Chen K L, Zhang H W, Gao C X, Wang D. Analysis of the functions of TaGW2 homoeologs in wheat grain weight and protein content traits. Plant Journal for Cell Molecular Biology, 2018, 94(5): 857-866
    [10] Bednarek J, Boulaflous A, Girousse C, Ravel C, Tassy C, Barret P, Bouzidi M F and Mouzeyar S. Down-regulation of the TaGW2 gene by RNA interference results in decreased grain size and weight in wheat. Journal of Experimental Botany, 2012, 63(16): 5945-5955.
    [11] Liu H, Li H F, Hao C Y, Wang K, Wang Y M, Qin L, An D G, Li T, Zhang X Y. TaDA1, a conserved negative regulator of kernel size, has an additive effect with TaGW2 in common wheat (Triticum aestivum L.). Plant Biotechnology Journal, 2019, 18(5): 1330-1342.
    [12] Zhang L, Zhao Y L, Gao L F, Zhao G Y, Zhou R H, Zhang B S, Jia J Z. TaCKX6-D1, the ortholog of rice OsCKX2, is associated with grain weight in hexaploid wheat. New Phytologist, 2012, 195(3): 574-584.
    [13] Wang S S, Zhang X F, Chen F, Cui D. A single-nucleotide polymorphism of TaGS5 gene revealed its association with kernel weight in Chinese bread wheat. Frontiers in Plant Science, 2015, 6(1166): 1166.
    [14] Ma L, Li T, Hao C Y, Wang Y Q, Chen X H, Zhang X Y. TaGS5-3A, a grain size gene selected during wheat improvement for larger kernel and yield. Plant Biotechnology Journal, 2016, 14:1269-1280.
    [15] Hou J, Li T,SWang Y M, SHao C Y, SLiu H X, SZhang X Y. ADP‐glucose pyrophosphorylase genes, associated with kernel weight, underwent selection during wheat domestication and breeding. Plant Biotechnology Journal, 2017, 15(12): 1533-1543.
    [16] Wang J Y, Wang R T, Mao X G, Zhang J L, Liu Y N, Xie Q, Yang X Y, Chang X P, Zhang X Y, Jing R L. RING finger ubiquitin E3 ligase gene TaSDIR1-4A contributes to grain size in common wheat. Journal of Experimental Botany, 2020, 71(18): 5377-5388.
    [17] Yang J, Zhou Y J, Wu Q H, Chen Y X, Zhang P P, Zhang Y, Hu W G, Wang X C, Zhao H, Dong L L, Han J, Liu Z Y, Cao T J. Molecular characterization of a novel TaGL3-5A allele and its association with grain length in wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 2019, 132:1799-1814
    [18] Bernard S M , Mller A L B , Dionisio G , Kichey T, Jahn T P, Dubois F, Baudo M M, Lopes M S, Tercé-Laforgue T, Foyer C H, Parry M A J, Forde B G, Araus J L, Hirel B, Schjoerring J K, Habash D. Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.). Plant Molecular Biology, 2008, 67(1-2): 89-105.
    [19] Ying G, Sun J J, Zhang G Z, Wang Y Y. Haplotype, molecular marker and phenotype effects associated with mineral nutrient and grain size traits of TaGS1a in wheat. Field Crops Research, 2013, 154(3): 119-125.
    [20] Ma M, Wang Q, Li Z J, Cheng H H, Li Z J, Liu X L, Song W N, Appels R, Zhao H X. Expression of TaCYP78A3, a gene encoding cytochrome P450 CYP78A3 protein in wheat (Triticum aestivum L.), affects seed size. Plant Journal, 2015, 83(2): 312-325.
    [21] Yang J, Zhou Y J, Zhang Y, Hu W G, Zhao H. Cloning, characterization of TaGS3 and identification of allelic variation associated with kernel traits in wheat (Triticum aestivum L.). BMC Genetics, 2019, 20: 98.
    [22] Hanif M, Gao F M, Liu J D, Wen W E, Zhang Y J, Rasheed A, Xia X C, He Z H, Cao S H. TaTGW6-A1, an ortholog of rice TGW6, is associated with grain weight and yield in bread wheat. Molecular Breeding, 2015, 36: 1.
    [23] Hu M J, Zhang H P, Cao J J, Zhu X F, Wang S X, Jiang H, Wu Z Y, Lu J, Chang C, Sun G L, Ma C X. Characterization of an IAA-glucose hydrolase gene TaTGW6 associated with grain weight in common wheat (Triticum aestivum L.). Molecular Breeding, 2016, 36(3): 25.
    [24] Wang W,SPan Q L,STian B,SHe F,SChen Y Y,SBai G H,SAkhunova A,STrick H N,SAkhunov E. Gene editing of the wheat homologs of TONNEAU1‐recruiting motif encoding gene affects grain shape and weight in wheat. Plant Journal, 2019, 100(2): 251-264
    [25] Yan X F, Zhao L, Ren Y, Z Dong D, Cui D Q, Chen F. Genome-wide association study revealed that the TaGW8 gene was associated with kernel size in Chinese bread wheat. Scientific Report, 2019, 9(1): 2702.
    [26] Qin L, Hao C Y, Hou J, Wang Y Q, Li T, Wang L F, Ma Z Q, Zhang X Y. Homologous haplotypes, expression, genetic effects and geographic distribution of the wheat yield gene TaGW2. BMC Plant Biology, 2014, 14(1): 107.
    [27] Wang S S, Yan X F, Wang Y Y, Liu H M, Cui D Q, Chen F. Haplotypes of the TaGS5-A1 Gene Are Associated with Thousand-Kernel Weight in Chinese Bread Wheat. Frontiers in Plant Science, 2016, 7(1046): 783.
    [28] 王沙沙, 黄 超, 汪庆昌, 晁岳恩, 陈 锋, 孙建国, 宋 晓. 小麦籽粒大小相关基因TaGS2克隆及功能分析. 作物学报, 2021, 已接收.Wang S S, Huang C, Wang Q C, Chao Y E, Chen F, Sun J G, Song X. Cloning and functional identification of TaGS2 gene related to kernel size in bread wheat. Acta Agronomica Sinica, 2021, accepted. (in Chinese)
    [29] Chen F, Gao M X, Zhang J H, Zuo A H, Shang X L, and Cui D Q. Molecular characterization of vernalization and response genes in bread wheat from the Yellow and Huai Valley of China. BMC Plant Biology, 2013, 13: 199.
    [30] Chen F, Li H, and Cui D Q. Discovery, distribution and diversity of Puroindoline-D1 genes in bread wheat from five countries (Triticum aestivum L.). BMC Plant Biology, 2013, 13: 125.
    [31] Chen, F., Xu, H. X., Zhang, F. Y., Xia, X. C., He, Z. H., Wang, D. W., et al. (2010). Physical mapping of puroindoline b-2 genes and molecular characterization of a novel variant in durum wheat (Triticum turgidum L.). Molecular Breeding. 2011, 28(2): 153-161.
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History
  • Received:January 28,2022
  • Revised:April 23,2022
  • Adopted:May 13,2022
  • Online: September 09,2022
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