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Home > Archive>Volume 23, Issue 5, 2022 >1425-1437. DOI:10.13430/j.cnki.jpgr.20220321003
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Genome-wide Association Analysis of Cold Tolerance Germination in Rice Core Germplasm of Guangdong Province in China
Author:
Affiliation:

Rice Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory

Fund Project:

Special Program for Crop Germplasm Resources of Guangdong Province（Governor's Special Program 2018-2019），The Investment Project of Department of Agriculture and Rural Affairs, Development and Reform Commission, Guangdong province（No. 272 of 2021），The Special Fund for Scientific Innovation Strategy-Construction of High Level Academy of Agriculture Science（No. R2020PY-JX001），Guangdong Key Laboratory of New Technology in Rice Breeding（2020B1212060047），The Key Field Research and Development Project of Guangdong Province（2020B0202090003）

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    Abstract:

    The purpose of this study is to identify the QTL associating with low temperature germination（LTG）in rice and screen germplasm resources showing strong cold tolerance during germination, laying a foundation for cold-tolerant germination-related molecular marker-assisted breeding. Fifty-seven rice varieties with strong cold tolerance during germination were obtained by counting the LTG rate of 309 rice core germplasm resources from Guangdong province, China. Using the LTG rate in conjugation with high-density SNP markers, eleven QTL were identified by genome-wide association scanning (GWAS), of which qLTG2-1, qLTG7-1, qLTG7-2 and qLTG10-1 were resided with previously-identified QTL. qLTG11-1, as the most significant newly-identified QTL, was subjected for RNA-seq and qRT-PCR, in order to predict its candidate genes. LOC_Os11g07020, which is found surrounding qLTG11-1, showed different expression patterns in varieties with high and low germination rates during LTG. This gene encodes for a fructose-1,6-bisphosphate aldolase isozyme, which may regulate glycolysis reaction to affect cold tolerance during germination. Collectively, this study identied 57 rice varieties with strong cold tolerance during germination as well as 11 QTL for LTG, which laid a theoretical foundation for molecular marker-assisted breeding of cold tolerance at germination stage.

    Reference
    [1] 王丽艳,唐金敏,郑桂萍,李红宇,钱永德,吕艳东,郭永霞,荆瑞勇. 水稻萌发期和幼苗期耐低温指标体系构建及综合评价. 中国农业科技导报,2019,21(10)：58-65Wang L Y,Tang J M,Zheng G P,Li H Y,Qian Y D,LYU Y D,Guo Y X,Jing R Y. Characterization and evaluation of low temperature resistance of rice at germination and seedling stages. Journal of Agricultural Science and Technology,2019,21(10)：58-65
    [2] Mahender A,Anandan A,Pradhan S K. Early seedling vigour, an imperative trait for direct-seeded rice: an overview on physio-morphological parameters and molecular markers,Planta,2015,241(5)：1027-1050
    [3] Fujino K,Sekiguchi H,Sato T,Kiuchi H,Nonoue Y,Takeuchi Y,Ando T,Lin S Y,Yano M. Mapping of quantitative trait loci controlling low-temperature germinability in rice(Oryza sativa L.). Theoretical and Applied Genetics,2004,108：794-799
    [4] 姜心禄,杨永波,付明全,李旭毅,池忠志,郑家国. 不同直播方式下种子处理方式对直播稻出苗和产量的影响. 中国稻米,2017,23(4)：111-114Jiang X L,Yang Y B,Fu M Q,Li X Y,Chi Z G,Zheng J G. Effects of seed treatments on seedling emergence and yield of direct seeding rice under different direct seeding methods. China Rice,2017,23(4)：111-114
    [5] 曹微,王燕,谭斌,刘伟,储莉,潘招远,赵光苗,曹桂元,周玉亮. 水稻品种直播相关的种子低温和低氧萌发活力评价. 分子植物育种,2018,16(10)：3259-3268Cao W,Wang Y,Tan B,Liu W,Chu L,Pan Z Y,Zhao G M,Cao G Y,Zhou Y L. Evaluation of seed germination vigor of rice cultivars under low temperature or hypoxic condition related with direct seeding rice. Molecular Plant Breeding,2018,16(10)：3259-3268
    [6] 蔡志欢,张桂莲. 水稻低温冷害研究进展. 作物研究,2018,32(3)：249-255Cai Z H,Zhang G L. Research progress of low temperature and chilling injury of rice. Crop Research,2018,32(3)：249-255
    [7] 滕胜,曾大力,钱前,国广泰史,黄大年,朱立煌. 低温条件下水稻发芽力QTL的定位分析. 科学通报,2001,46(13)：1104-1108Teng S,Zeng D L,Qian Q,Kunihiro Y,Huang D N,Zhu L H. QTL analysis of germinability under low temperature in rice. Chinese Science Bulletin,2001,46(13)：1104-1108
    [8] 姜旋,李辰昱,毛婷. 水稻低温发芽性QTL的分子标记定位. 武汉植物学研究,2005,23(3)：216-220Jiang X,Li C Y,Mao T. Mapping of QTL controlling low-temperature germinability in rice. Journal of Wuhan Botanical Research,2005,23(3)：216-220
    [9] 巩迎军,阮雯君,荀星,董彦君,林冬枝,叶胜海,张小明. 水稻芽性状耐冷性的QTL分析. 分子植物育种,2009,7(2)：273-278Gong Y J,Ruan W J,Gou X,Dong Y J,Lin D Z,Ye S H,Zhang X M. QTL analysis of cold tolerance for two bud traits in rice. Molecular Plant Breeding,2009,7(2)：273-278
    [10] Li L,Liu X,Xie K,Wang Y,Liu F,Lin Q,Wang W,Yang C,Lu B,Liu S,Chen L,Jiang L,Wan J. qLTG-9, a stable quantitative trait locus for low-temperature germination in rice(Oryza sativa L.). Theoretical and Applied Genetics,2013,126：2313-2322
    [11] Fujino K,Sekiguchi H,Matsuda Y,Sugimoto K,Ono K,Yano M. Molecular identification of a major quantitative trait locus, qLTG3-1, controlling low-temperature germinability in rice. Proceedings of the National Academy of Sciences of the United States of America,2008,105(34)：12623-12628
    [12] Wang X,Zou B,Shao Q,Cui Y,Lu S,Zhang Y,Huang Q,Huang J,Hua J. Natural variation reveals that OsSAP16 controls low-temperature germination in rice. Journal of Experimental Botany,2018,69(3)：413-421
    [13] 侯青青,司丽珍,黄学辉,韩斌. 水稻复杂性状研究的新途径：水稻重要农艺性状全基因组关联分析. 生命科学,2018,28(10)：1250-1257Hou Q Q,Si L Z,Huang X H,Han B. Progress on genome-wide association study of important agronomic traits in rice. Chinese Bulletin of Life Sciences,2018,28(10)：1250-1257
    [14] Sales E,Viruel J,Domingo C,Marques L. Genome wide association analysis of cold tolerance at germination in temperate japonica rice(Oryza sativa L.)varieties. PLOS ONE,2017,12(8)：e0183416
    [15] Pan Y H,Zhang H L,Zhang D L,Li J J,Xiong H Y,Yu J P,Li J L,Rashid M A R,Li G L,Ma X D,Cao G L,Han L Z,Li Z C. Genetic analysis of cold tolerance at the germination and booting stages in rice by association mapping. PLOS ONE,2015,10(3)：e0120590
    [16]Najeeb S,Ali J,Mahender A,Pang Y L,Zilhas J,Murugaiyan V,Vemireddy L R,Li Z. Identification of main-effect quantitative trait loci(QTLs)for low-temperature stress tolerance germination- and early seedling vigor-related traits in rice(Oryza sativa L.). Molecular Breeding,2020,40(10)
    [17] 马雅美,张少红,赵均良. 水稻直播相关性状遗传分析及分子机制研究进展. 广东农业科学,2021,48(10)：13-22Ma Y M,Zhang S H,Zhao J L. Research progress in genetic analysis and molecular mechanisms of rice direct-seeding related traits. Guangdong Agricultural Sciences,2021,48(10)：13-22
    [18] 胡巍,高云,张强,张彬,江奕君. 浅绿叶水稻突变体的特性与遗传分析. 植物遗传资源学报,2021,22(1)：194-204Hu W,Gao Y,Zhang Q,Zhang B,Jiang Y J. Genetic analysis and characteristics of pale-green leaf rice mutant. Journal of Plant Genetic Resources,2021,22(1)：194-204
    [19] 周新桥,陈达刚,李丽君,刘传光,张旭,陈友订. 秧苗期低温胁迫对华南主推双季水稻生长的影响. 西南农业学报,2013, 26(3)：936-941Zhou X Q,Chen D G,Li L J,Liu C G,Zhang X,Chen Y D. Effects of chilling stress on growth of double cropping rice in south China at seedling stage,2013,26(3)：936-941
    [20] 赵莹. 烟草果糖-1,6-二磷酸醛缩酶基因的系统鉴定、特性分析及其非生物胁迫响应研究. 泰安：山东农业大学,2020Zhao Y. Systematic identification, characteristic analysis and abiotic stress response research of fructose-1,6-bisphosphate aldolase genes in Nicotiana tabacum. TaiAn： Shandong Agricultural University,2020
    [21] 龙瑞才,杨青川,康俊梅,王凭青,秦智慧. 紫花苜蓿果糖-1,6-二磷酸醛缩酶基因全长克隆及分析. 西北植物学报,2010,30(6)：1075-1082Long R C,Yang Q C,Kang J M,Wang P Q,Qin Z H. Cloning and characterization of a fructose-1,6-bisphosphate aldolase gene in Medicago sativa L. Acta Botanica Boreali-Occidentalia Sinica,2010,30(6)：1075-1082
    [22] 张帆,常李伟,丁明全,侯佩臣,沈昕,陈少良. 胡杨果糖-1,6-二磷酸醛羧酶基因PeALD的克隆及耐盐性分析. 基因组学与应用生物学,2011,30(3)：257-267Zhang F,Chang L W,Ding M Q,Hou P C,Shen X,Chen S L. Cloning and salt-tolerant analysis of fructose-1,6-bisphosphate aldolase gene PeALD of Populus euphratica Oliv. Genomics and Applied Biology,2011,30(3)：257-267
    [23] Cai B B,Li Q,Xu Y C,Yang L,Bi H A,Ai X Z. Genome-wide analysis of the fructose 1,6-bisphosphate aldolase(FBA)gene family and functional characterization of FBA7 in tomato. Plant Physiology Biochemistry,2016,108：251-265
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History
  • Received:March 21,2022
  • Revised:April 18,2022
  • Adopted:June 08,2022
  • Online: September 09,2022
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