2025-4-6- 17
Home > Archive>Volume 23, Issue 5, 2022 >1310-1320. DOI:10.13430/j.cnki.jpgr.20220517007
PDF HTML XML Export Cite reminder
Identification of Clubroot Resistance and Molecular Markers Linked with CR Genes in Turnip (Brassica rapa L. ssp. rapifera) germplasm
Author:
Affiliation:

1.State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei/Collaborative Innovation Center of Vegetable Industry in Heibei/College of Horticulture, Hebei Agricultural University;2.Guoyan seed Co Ltd of Hebei Province

Fund Project:

Introduction of Foreign Intellectual Projects in Hebei Province(2019YX023A)，Hebei Innovation Capability Improvement Plan Project (20592901D)，Hebei Province

  • Article
    • | |
  • Metrics
    • |
  • Reference [30]
    • | | | |
  • Comments
    Abstract:

    Clubroot disease is one of the most important diseases of Chinese cabbage and other cruciferous crops. Identification of new clubroot resistant resources is of importance in breeding for resistant varieties to effectively prevent the occurrence. In this study, two resistant and two susceptible inbred lines of Chinese cabbage, respectively, were used to identify the polymorphic markers that are closely linked to the CR genes of the resistant inbred lines. Sixty-three turnip germplasms were tested for resistance at the seedling stage by inoculation using the bacterium Plasmodiophora brassicae isolates from Wulong district of Chongqing municipality and Tangshan city of Hebei province. The turnip germplasms showing resistant or tolerant were genotyped using molecular markers linked to CR genes. Based on 20 tested markers, two were found to be linked to the resistance gene CRa, one marker linked to CRb, five markers linked to CRbkato and one marker linked to CRd. Six turnip germplasms showing good resistance simultaneously to isolates were collected from Centre for Genetic Resources (CGN), Netherlands, including three forage turnips and three vegetable turnips originally from European countries. The genotyping results also suggested a heterozygous status in the genetic background of these turnips. The germplasms CGN1, CGN10 and CGN11 were found harboring four CR loci (CRa, CRbkato, CRb and CRd), CGN23 and CGN36 were detected harboring three loci (CRa, CRbkato and CRd), and CGN30 carried two CR loci (CRa and CRbkato). Whether these turnip germplasms host additional CR loci still remains to be further investigated. Collectively, these results provided materials for mining new CR genes and breeding new CR varieties of Chinese cabbage and other cruciferous crops.

    Reference
    [1] 原玉香, 赵艳艳, 魏小春, 姚秋菊, 蒋武生, 王志勇, 李扬, 许茜, 杨双娟, 张晓伟. 河南省大白菜根肿病菌生理小种鉴定. 河南农业科学, 2017, 46(7): 71-76.Yuan Y X, Zhao Y Y, Wei X C, Yao Q J, Jiang W S, Wang Z Y, Li Y, Xu Q, Yang S J, Zhang X W. Pathotype identification of Plasmodiophora brassicae Woron. collected from Chinese cabbage in Henan Province[J]. Journal of Henan Agricultural Sciences, 2017, 46(7): 71-76.
    [2]Matsumoto E, Yasui C, Ohi M, Tsukada M. Linkage analysis of RFLP markers for clubroot resistance and pigmentation in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Euphytica, 1998, 104: 79-86.
    [3]Chen J J, Jing J, Zhan Z X, Zhang T, Zhang C Y, Piao Z Y. Identification of novel QTLs for isolate-specific partial resistance to Plasmodiophora brassicae in Brassica rapa. Plos One, 2013, 8: e85307.
    [4]Hatakeyama K, Suwabe K, Tomita R N, Kato T, Nunome T, Fukuoka H, Matsumoto S. Identification and characterization of Crr1a, a gene for resistance to clubroot disease (Plasmodiophora brassicae Woronin) in Brassica rapa L. Plos One, 2013, 8(1): e54745.
    [5] Chang A, Lamara M, Wei Y, Hu H, Parkin I A P, Gossen B D, Peng G, Yu F. Clubroot resistance gene Rcr6 in Brassica nigra resides in a genomic region homologous to chromosome A08 in B. rapa. BMC Plant Biology, 2019, 19, 224.
    [6] Pang W X, Fu P Y, Li X N, Zhan Z X, Yu S, Piao Z Y. Identification and mapping of the clubroot resistance gene CRd in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Frontiers in Plant Science, 2018, 9: 653.
    [7] Huang Z, Peng G, Liu X J, Deora A, Falk K C, Gossen B D, Mary R, Yu F. Fine mapping of a clubroot resistance gene from turnip using SNP markers identified from bulked segregant RNA-Seq. Frontiers in Plant Science 2017, 8: doi: 10.3389/fpls.2017.01448
    [8] Piao Z Y, Deng Y Q, Choi S R, Park Y J, Lim Y P. SCAR and CAPS mapping of CRb, a gene conferring resistance to Plasmodiophora brassicae in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Theoretical and Applied Genetics, 2004, 108: 1458-1465.
    [9] Sakamoto K, Saito A, Hayashida N, Taguchi G, Matsumoto E. Mapping of isolate-specific QTL for clubroot resistance in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Theoretical and Applied Genetics, 2008, 117: 759-767.
    [10] Chu M G, Song T, Falk K C, Zhang X G,Liu X J, Chang A, Lahlali R, McGregor L, Gossen B D, Peng G, Yu F Q. Fine mapping of Rcr1 and analyses of it’s effect on transcriptome patterns during infection by Plasmodiophora brassicae. BMC Genomics, 2014, 15(1): 1166.
    [11] Hirai M, Harada T, Kubo N, Tsukada M, Suwabe K, Matsumoto S. A novel locus for clubroot resistance in Brassica rapa and its linkage markers. Theoretical and Applied Genetics, 2004, 108: 639-643.
    [12] Kato T, Hatakeyama K, Fukino N, Matumoto S. Identificaiton of a clubroot resistance locus conferring resistance to a Plasmodiophora brassicae classified into pathotype group 3 in Chinese cabbage (Brassica rapa L.). Breeding Science, 2012, 62(3): 282-287.
    [13] Kato T, Hatakeyama K, Fukino N, Matumoto S. Fine mapping of the clubroot resistance gene CRb and development of a useful selectable marker in Brassica rapa. Breeding Science, 2013, 63(1): 116-124.
    [14] Suwabe K, Suzuki G, Nunome T, Hatakeyama K, Mukai Y, Fukuoka H, Matsumoto S. Microstructure of the Brassica rapa genome segment that are homeologous to resistance gene cluster in Arabidopsis chromosome 4. Breeding Science, 2012, 62: 170-177.
    [15]Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Fujimura M, Nunome T, Fukuoka H, Hirai M. Identification of two loci for resistance to clubroot (Plasmodiophora brassicae Woronin) in Brassica rapa L. Theoretical and Applied Genetics, 2003, 107: 997-1002.
    [16]Suwabe K, Tsukada H, Iketani H, Hatakeyama K, Kondo M, Fujimura M, Nunome M, Fukuoka H, Hirai M, Matsumoto S. Simple sequence repeat based comparative genomics between Brassica rapa and Arabidopsis thaliana: the genetic origin of clubroot resistance. Genetics, 2006, 173: 309-319.
    [17] Strelkov S E, Hwang S F, Manolii V P, Cao T S, Fredua-Agyeman R, Harding M W, Peng G, Gossen B D, Mcdonald M R, Feindel D. Virulence and pathotype classification of Plasmodiophora brassicae populations collected from clubroot resistant canola (Brassica napus) in Canada. Canadian Journal of Plant Pathology, 2019, 40, 284-298.
    [18] Zhan Z, Jiang Y, Shah N, Hou Z, Zhou Y, Dun B, Li S, Zhu L, Li Z, Piao Z, Zhang C. Association of clubroot resistance locus PbBa8.1 with a linkage drag of high erucic acid content in the seed of the European turnip. Frontiers inSPlantSScience, 2020, 11: 810.
    [19] Ren W J, Li Z Y, Han F Q, Zhang B, Li X, Fang Z Y, Yang L M, Zhuang M, Lv HH, Liu Y M, Wang Y, Yu H, Zhang YY. Utilization of Ogura CMS germplasm with the clubroot resistance gene by fertility restoration and cytoplasm replacement in Brassica oleracea L. Horticulture Research, 2020, 7(1): 61.
    [20] Hatakeyama K, Fujimura M, Ishida M, Suzuki T, Sato . Classification of pathogenicity ofSPlasmodiophora brassicaeSfield isolates in Japan based on resistance of F1Scultivars of Chinese cabbage (Brassica rapaSL.) to clubroot. Acta Horticulturae, 2006, (706): 323-328.
    [21] 丁云花, 简元才, 余阳俊, 汪维红, 耿丽华, 康俊根. 我国8市十字花科蔬菜根肿病菌生理小种的鉴定. 中国蔬菜, 2013, (16): 85-88.Ding Y H, Jian Y C, Yu Y J, Wang W H, Geng L H, Kang J G. Identification of pathotype of Plasmodiophora brassicae on Crucifer vegetables. Chinese vegetable, 2013, (16): 85-88.
    [22] Hayashida1 N, Takabatake Y, Nakazawa N, Aruga D, Nakanishi H, Taguchi G, Sakamoto K, Matsumoto E. Construction of a practical SCAR marker linked to clubroot resistance in Chinese cabbage, with intensive analysis of HC352b genes. Journal of the Japanese Society for Horticultural Science, 2008, 77 (2): 150–154.
    [23] Ueno H, Matsumoto E, Aruga D, Kitagawa S, Matsumura H, Hayashida N. Molecular characterization of the CRa gene conferring clubroot resistance in Brassica rapa. Plant Molecular Biology, 2012, 80(6): 621-629.
    [24] 陈慧慧, 张腾, 梁珊, 陈兵, 张椿雨, 朴钟云. 大白菜抗根肿病 CRb 基因紧密连锁标记的开发与定位. 中国农业科学, 2012, 45(17): 3551-3557.Chen H H, Zhang T, Liang S, Chen B, Zhang C Y, Piao Z Y. Development and mapping of molecular markers closely linked to CRb gene resistance to clubroot disease in Chinese cabbage . Scientia Agricultura Sinica, 2012, 45(17): 3551-3557.
    [25] Zhang T, Zhao Z, Zhang C Y, Pang W X, Choi S R, Lim Y P, Piao Z Y. Fine genetic and physical mapping of the CRb gene conferring resistance to clubroot disease in Brassica rapa. Molecular Breeding, 2014, 34(3): 1173-1183.
    [26] 朱东旭, 王彦华, 赵建军, 刘博, 李晓峰, 轩淑欣, 申书兴. 结球甘蓝相对于大白菜连锁群特异InDel标记的建立及应用. 园艺学报, 2014, 41(8): 1699-1706.Zhu D X, Wang Y H, Zhao J J, Liu B,Li X F, Xuan S X, Shen S X. Establishment and application of specific InDel markers on different linkage groups of cabbage compared with Chinese cabbage. Acta Horticulturae Sinica, 2014, 41(8): 1699-1706.
    [27] 王丽丽, 王鑫, 吴海东, 何明, 李颖. 我国主要抗根肿病大白菜品种抗性鉴定及评价. 中国蔬菜, 2017, (8): 46-50.Wang L L, Wang X, Wu H D, He M, Li Y. Resistance identification and evaluation of clubroot resistant varieties of Chinese cabbage to Plasmodiophora brassicae. China vegetables, 2017, (8): 46-50.
    [28] Yang Z Q, Jiang Y F, Gong J F, Li Q, Dun B C, Liu D X, Yin F F, Yuan L, Zhou X Q, Wang H Y, Wang J, Zhan Z X, Shah N, Nwafor C C, Zhou Y W, Chen P, Zhu L, Li S S, Wang B R, Xiang J, Zhou Y M, Li Z Y, Piao Z Y, Yang Q Y, Zhang C Y. R gene triplication confers European fodder turnip with improved clubroot resistance. Plant Biotechnology Journal, 2022, https://doi.org/10.1111/pbi.13827.
    [29] 孙朝辉, 马安峰, 曹丹丹, 程斐, 高建伟. 大白菜种质资源抗根肿病基因CRa和CRb的分子标记鉴定与分析. 山东农业科学, 2016, 48(12): 16-19+53.Sun Z H, Ma A F, Cao D D, Cheng F, Gao J W. Identification of CRa and CRb genes resistant to clubroot disease among twenty-four Chinese cabbage varieties with molecular markers. Shandong Agricultural Sciences, 2016, 48(12): 16-19+53.
    [30] 任平平, 马安峰, 程斐, 孙朝辉, 高建伟. 大白菜抗根肿病基因CRb的分子标记验证与种质资源筛选. 山东农业科学, 2016, 48(6) : 7-10.Ren P P, Ma A F, Cheng F, Sun Z H, Gao J W. Identification of molecular markers forclubroot disease resistant gene CRb and screening of germplasm resources of Chinese cabbage. Shandong Agricultural Sciences, 2016, 48(6) : 7-10.
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

Copy
Share
Article Metrics
  • Abstract:317
  • PDF: 1736
  • HTML: 0
  • Cited by: 0
History
  • Received:May 17,2022
  • Revised:June 20,2022
  • Adopted:June 28,2022
  • Online: September 09,2022
Article QR Code
You are the 606195th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.