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Home > Archive>Volume 23, Issue 5, 2022 >1258-1268. DOI:10.13430/j.cnki.jpgr.20220527002
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Study on Cryopreservation Method of Allium Crop Germplasm
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Affiliation:

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crop Biology and Germplasm Creation in the Ministry of Agriculture

Fund Project:

National Modern Agricultural Industry Technology System Construction Special Fund Project (CARS-24-A-01)，National Natural Science Foundation of China (31872946)，Innovation Engineering Project of Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2017-IVFCAAS)，Ministry of Agriculture and Rural Affairs Crop Germplasm Resources Safety Conservation Project (2022NWB037)，National Horticultural Germplasm Beijing Vegetable Sub-bank Operation Service Project (NHGRC2022-NH01)，Observation and Monitoring Mission Project of National Crop Germplasm Resources Data Center (ZX01NK-03)

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    Abstract:

    Allium crop germplasm is ephemeral vegetable due to its short-lived seeds. Application of the conventional low-temperature preservation is difficult to achieve long-term preservation. It is therefore of great significance to set up the cryopreservation procedure specific to the allium germplasm. In the present study, through the orthogonal experiment of three factors with four levels, we examined the cryopreservation methods using 30 accessions of four allium vegetables (Allium tuberosum Rottler ex Sprengle, Allium fistulosum L. var. giganteum Makino, Allium porrum L. and Allium cepa L.). These results was evaluated by the germination rate of the seeds after cryopreservation if compared to the germination rate of the seeds without treatments. The results showed significant differences among the ANOVA experiments (P<0.05), while the optimal procedure was the A1B4C1 (without cryoprotectant PVS2 treatment, storage in liquid nitrogen, and rewarming for 4 h at 40°C before testing the germination rate). We also determined the physiological indexes of superoxide dismutase (SOD), peroxidase (POD) activity, catalase (CAT) activity, malondialdehyde (MDA) content of cryopreservation seeds after seed rewarming. The MDA content of the seeds decreased after ultra-low temperature preservation of liquid nitrogen, while the SOD activity was higher than that of the control after the liquid nitrogen treatment. A decrease on the POD activity of A. tuberosum Rottler ex Sprengle, after liquid nitrogen treatment and an increase on the POD activity of A. porrum L., A. fistulosum L. var. giganteum Makino and A. cepa L. were detected. Collectively, these results provided optimal cryopreservation procedure for Allium vegetable germplasm resources.

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History
  • Received:May 27,2022
  • Revised:June 22,2022
  • Adopted:June 28,2022
  • Online: September 09,2022
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