488 Malus germplasm resources, including cultivates, wild species, ancient cultivates and ornamental crabapple cultivar, were evaluated for fire blight resistance by in vitro inoculating young leaves and shoots. A difference on resistance revealed by two inoculation methods (leaves vs. shoots) was observed, and a higher proportion of germplasms showing resistance were detected by inoculating the young shoots. The germplasm of different species, origins and pedigrees represented significant different on disease resistance. The results of young leaves inoculation showed that the proportion of resistant resources in cultivates and wild species was higher than that in ancient cultivates. The proportion of resistant resources of McIntosh strains and Golden Delicious strains was higher than that of other strains. By analyzing the passport information of 189 Malus sieversii resources, these resources showing resistant were mainly from Uzbekistan and Xinyuan County of Xinjiang autonomous region, China. The young shoots inoculation revealed a similar pattern with young leaves inoculation except for Malus sieversii, which had higher proportion of resistant resources from Uzbekistan and Gongliu County of Xinjiang autonomous region, China. There were 187 Malus germplasm resource showing consistent results revealed by both inoculation methods, of which the McIntosh strains showed strong resistance, and majority of the Malus sieversii resources showing resistant were collected from Uzbekistan and Xinyuan County of Xinjiang autonomous region, China. The eight Malus germplasm resources were observed with high ressitance against fire blight, including six cultivates that could be used as the parents for germplasm innovation and cultivar breeding, as well s two Malus sieversii resources that could be used as basic materials for cultivar and rootstocks breeding.