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Home > Archive>Volume 24, Issue 1, 2023 >215-225. DOI:10.13430/j.cnki.jpgr.20220728001
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Isolation of SFB Genes of 82 Malus Germplasm Resources and Gene Frequency Analysis of Different Ecological Populations
Author:
Affiliation:

1.Research Institute of Pomology ， Chinese Academy of Agricultural Sciences / Key Laboratory of Horticultural Crops Germplasm Resources Utilization， Ministry of Agriculture and Rural Affairs，Liaoning Xingcheng 125100;2.College of Agriculture， Shihezi University / Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization， Xinjiang Shihezi 832000

Fund Project:

Foundation project： The Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences （CAAS-ASTIP-2021-RIP-02）

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    Abstract:

    In order to clarify the SFB gene diversity in Malus Mill. and the distribution frequency of each sequence haplotype in ecological populations， 82 Malus Mill. germplasm resources including the cultivated apples （i.e. Kuihua） and wild resources （i.e. Malus sieversii（Ledeb.） M. Roem.） were used. The genotyping was conducted using three gene-specific primer combinations. PCR amplification using leaf DNA produced a single SFB gene fragment in all materials. Sanger sequencing of PCR products revealed these fragments belonged to five different SFB genes， corresponding SFB1， SFB2， SFB3， SFB7and SFB9 based on GenBank homology comparison. At the same time， the distribution frequency of SFB gene in different ecological population was analyzed. The frequencies of different haplotypes in populations were different， and the highest frequency of SFB3 was 43.90%， followed by SFB1 （39.00%）. The intraspecific and interspecific frequency of the same SFB gene was also different. In addition， the phylogenetic tree analysis showed that Malus sieversii （Ledeb.） M. Roem. was related to the domestication and origin of Malus turkmenorumJuz. & M. Pop. and apple cultivars.

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History
  • Received:July 28,2022
  • Revised:August 17,2022
  • Online: January 12,2023
  • Published: January 12,2023
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