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Home > Archive>Volume 24, Issue 1, 2023 >296-306. DOI:10.13430/j.cnki.jpgr.20220811001
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Transcriptome Analysis of Cold-treated Tetraploid Yellow-fleshed Actinidia chinensis Planch.
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Affiliation:

Key Laboratory of Forest Genetic Improvement and Breeding/The Key Laboratory of Biodiversity Conservation of Southwest China， National Forestry and Grassland Administration， College of Forestry， Southwest Forestry University， Kunming 650224

Fund Project:

Foundation project： National Natural Science Foundation of China （32160556）

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    Abstract:

    Hort16A， a variety of Actinidia chinensis Planch.， is highly popular among consumers due to its high nutritional value. However， this variety is sensitive to frost and susceptible to ulcer disease and branch rot. The response mechanism of yellow-fleshed A. chinensis upon cold temperature remains still unclear. In this study， transcriptome analysis of yellow-fleshed kiwifruit under low temperature stress was performed to provide theoretical references for understanding the mechanisms involved and subsequent studies on cold resistance. The cold-resistant tetraploid materials and the plants treated at low temperature （0 ℃） for 5 h were used as the experimental group， and those not specially treated at room temperature were used as the control group， and the samples were taken for transcriptome sequencing using Illumina NovaSeq 6000 platform. GO enrichment analysis revealed 1013 differentially expressed genes， which were enriched into three categories： molecular function， cellular component， and biological process. KEGG enrichment analysis showed that 410 differentially expressed genes were enriched in the three main functions of plant hormone signal transduction， MAPK signaling pathway-plant， starch and sucrose metabolism， and 89 other metabolic pathways. Nine genes related to cold resistance in yellow-fleshed kiwifruit， including PYL， PSR， TPS， GH3， SAUR， and PP2C， were tentatively identified. The results of this study can provide theoretical references for further studies on the response mechanism of tetraploid yellow-fleshed kiwifruit to low temperature stress.

    Reference
    [1] 廖慧苹,梅兵,鲍荣粉,罗中魏,叶青. 中国猕猴桃资源的利用与品种开发研究. 落叶果树,2018,50(3):33-35Liao H P, Mei B, Bao R F, Luo Z W, Ye Q. Utilization and variety development of Actinidia chinensis resources in China. Deciduous Fruits, 2018,50(3):33-35
    [2] 孙雷明,方金豹. 我国猕猴桃种质资源的保存与研究利用. 植物遗传资源学报, 2020, 21(6): 1483-1493Sun L M, Fang J B. Conservation, research and utilization of kiwifruit germplasm resources in China. Journal of Plant Genetic Resources, 2020, 21(6): 1483-1493
    [3] 刘殊. 猕猴桃的冻害及其预防. 中国农资, 1999(4): 25Liu S. Frost damage in kiwifruit and its prevention.China Agri-Production News, 1999(4): 25
    [4] 王利虎,卢彦琦,苏行,张琼,赵志军,陈敬谊,丁保朋. 果树多倍化育种研究进展. 山西农业大学学报:自然科学版, 2022, 42(3): 14-24Wang L H, Lu Y Q, Su H, Zhang Q, Zhao Z J, Chen J Y, Ding B P. Research progress on polyploidy breeding of fruit trees. Journal of Shanxi Agricultural University: Natural Sciences Edition, 2022, 42(3): 14-24
    [5] 张宏宇,朱心慰,侯鹏奇,胡锐峰,黄玺,侯义龙. 果树单倍体和多倍体研究进展. 分子植物育种, 2019, 17(2): 606-611Zhang H Y, Zhu X W, Hou P Q, Hu R F, Huang X, Hou Y L. Research progress and prospect of fruit tree haploid and polyploid. Molecular Plant Breeding, 2019, 17(2): 606-611
    [6] 李智奕,宁维,陈利平,李瑜,韩亚伟,史媛媛. 新一代测序技术及其在植物转录组研究中的应用. 河南农业科学, 2013 (12): 1-5Li Z Y, Ning W, Chen L P, Li Y, Han Y W, Shi Y Y. The next-generation sequencing technology and its application in plant transcriptome. Journal of Henan Agricultural Sciences, 2013(12): 1-5
    [7] 王莹. 云雾贡茶低温胁迫下的转录组分析及CsPPO基因的功能鉴定. 贵阳:贵州大学, 2021Wang Y. Transcriptome analysis and functional identification of CsPPO gene under low temperature stress in Yunwu tribute tea. Guiyang: Guizhou University, 2021
    [8] 梁雨晨,蔺海娇,侯一航,李玲,王芊乔,付畅,苑泽宁. 狭叶薰衣草叶片转录组耐寒相关差异表达基因分析. 分子植物育种, 2021, URL: http://kns.cnki.net/kcms/detail/46.1068.S.20210604.1045.010.htmlLiang Y C, Lin H J, Hou Y H, Li L, Wang Q Q, Fu C, Yuan Z N. Analysis of differentially expressed genes related to cold tolerance in the leaf transcriptome of Lavandula angustifolia Mill. Molecular Plant Breeding, 2021, URL: http://kns.cnki.net/kcms/detail/46.1068.S.20210604.1045.010.html
    [9] 刘奕清. 低温胁迫下两种桉树的生理响应特征及转录表达差异研究. 北京:中国农业大学, 2015Liu Y Q. Physiological response characteristics and transcriptomic comparsion of two Eucalyptu trees under low temperature stress. Beijing: China Agricultural University, 2015
    [10] Li S, Liu X, Liu H, Zhang X, Ye Q, Zhang H. Induction, identification and genetics analysis of tetraploid Actinidia chinensis. Royal Society Open Science, 2019, 6: 191052
    [11] 刘粉香,杨文国,孙勤红. 基于转录组测序数据分析及高通量GO注释理论的研究. 安徽农业科学, 2018, 46(31): 88-91Liu F X, Yang W G, Sun Q H. Transcriptome sequencing data analysis and high-throughput GO annotation. Journal of Anhui Agricultural Sciences, 2018, 46(31): 88-91
    [12] 周贺,李浩男,蔡斌华,乔玉山. 砂梨果皮转录组SNP位点发掘及其功能注释分析. 青岛农业大学学报:自然科学版, 2014, 31(2): 105-111Zhou H, Li H N, Cai B H, Qiao Y S. SNP mining and functional annotation in transcriptome of sand pear. Journal of Qingdao Agricultural University: Natural Science, 2014, 31(2): 105-111
    [13] 田玉珍,党兆霞,吕前前,毛娟,褚明宇,马宗桓,左存武,陈佰鸿. 树体休眠期前苹果花芽对低温早期响应的转录组分析. 果树学报, 2020, 37(5): 615-624Tian Y Z, Dang Z X, Lü Q Q, Mao J, Chu M Y, Ma Z H, Zuo C W, Chen B H. Transcriptomic analysis of early responses of apple flower buds to low temperature before tree dormancy. Journal of Fruit Science, 2020, 37(5): 615-624
    [14] 林俐,吴健. 植物MAPK级联在逆境胁迫响应中的作用研究进展. 分子植物育种, 2018, 16(1): 280-288Lin L, Wu J. Progress in plant MAPK cascades in response to environmental stresses. Molecular Plant Breeding, 2018, 16(1), 280-288
    [15] Fan M L, Zhang Y, Li X L, Wu S, Yang M Y, Yin H F, Liu W X, Fan Z Q, Li J Y. Multi-approach analysis reveals pathways of cold tolerance divergence in Camellia japonica. Frontiers in Plant Science, 2022, 13:811791
    [16] 高洁. 黄肉猕猴桃果实发育过程中色素变化及转录组与转色期DGE分析. 南昌:江西农业大学, 2013Gao J. Studies on variation of the flesh pigments and analysis of transcriptome and DGE in color turning phases of yellow kiwifruit. Nanchang: Jiangxi Agricultural University, 2013
    [17] 涂美艳,李明章,孙淑霞,李靖,陈栋,宋海岩,江国良,廖明安. 黄肉猕猴桃中果皮和内果皮不同时期比较转录组分析. 分子植物育种, 2019, 17(5): 1479-1486Tu M Y, Li M Z, Sun S X, Li J, Chen D, Song H Y, Jiang G L, Liao M A. Comparative transcriptome analysis of different development stages of yellow-fleshed kiwifruit sarcocarp and endocarp. Molecular Plant Breeding, 2019, 17(5): 1479-1486
    [18] Ding X H, Cao Y L, Huang L L, Zhao J, Xu C G, Li X H, Wang S P. Activation of the indole-3-acetic acid-amido synthetase GH3-8 suppresses expansin expression and promotes salicylate- and jasmonate-independent basal immunity in rice. Plant Cell, 2008 ,20(1):228-240
    [19] 张计育,渠慎春,郭忠仁,杜小丽,都贝贝,章镇. 植物bZIP转录因子的生物学功能. 西北植物学报, 2011, 31(5): 1066-1075Zhang J Y, Qu S C, Guo Z R, Du X L, Du B B, Zhang Z. Biology function of bZIP transcription factors in plants . Acta Botanica Boreali-Occidentalia Sinica , 2011, 31(5): 1066-1075
    [20] Cai W, Yang Y, Wang W, Guo G, Liu W, Bi C. Overexpression of a wheat (Triticum aestivum L.) bZIP transcription factor gene, TabZIP6, decreased the freezing tolerance of transgenic Arabidopsis seedlings by down-regulating the expression of CBFs. Plant Physiol Biochem. 2018,124:100-111
    [21] 李傲,崔梦杰,陈珂,许瀛之,贾海峰,房经贵. 葡萄SAUR基因家族鉴定与生物信息学分析. 植物遗传资源学报, 2018, 19(2): 326-337Li A, Cui M J, Chen K, Xu Y Z, Jia H F, Fang J G. Identification and bioinformatics analysis of the SAUＲ gene family in grape. Journal of Plant Genetic Ｒesources, 2018, 19(2): 326-337
    [22] 潘巧文,武军艳,马学才,王亚林,马骊,徐新宇,李鹏,姚彦林,徐芳,崔小茹,孙万仓. 白菜型油菜BraSAUR基因家族鉴定及其表达分析. 干旱地区农业研究, 2022, 40(5): 1-12,23Pan Q W, Wu J Y, Ma X C, Wang Y L, Ma L, Xu X Y, Li P, Yao Y L, Xu F, Cui X R, Sun W C. Identification and expression analysis of BraSAUR gene family in rapeseed (Brassica rapa L.). Agricultural Research in the Arid Areas, 2022, 40(5): 1-12,23
    [23] Du H, Wu N, Fu J, Wang S P, Li X H, Xiao J H, Xiong L Z. A GH3 family member, OsGH3-2, modulates auxin and abscisic acid levels and differentially affects drought and cold tolerance in rice. Journal of Experimental Botany, 2012, 63(18): 6467-6480
    [24] 夏全超,张玉霞,孙明雪,张庆昕,杜晓艳,王显国,王东儒. 施磷深度对低温胁迫苜蓿根颈糖类物质含量及抗寒性的影响. 西北农林科技大学学报:自然科学版, 2022(11): 2-9Xia Q C, Zhang Y X, Sun M X, Zhang Q X, Du X Y, Wang X G, Wang D R. Effects of phosphate fertilizer application depth on carbohydrate content and cold resistance of alfalfa root crown under low temperature stress. Journal of Northwest A&F University: Natural Science Edition, 2022(11): 2-9
    [25] Peng T, Zhu X F, Duan N, Liu J H. PtrBAM1, a β-amylase-coding gene of Poncirus trifoliata, is a CBF regulon member with function in cold tolerance by modulating soluble sugar levels. Plant Cell and Environment, 2014, 37(12), 2754-2767
    [26] Knaupp M , Mishra K B, Nedbal L, Heyer A G. Evidence for a role of raf-finose in stabilizing photosystem II during freeze?thaw cycles. Planta, 2011, 234: 477-486
    [27] 王彩丽. 棉花海藻糖6-磷酸合酶11和3个LEA基因的功能鉴定. 泰安:山东农业大学, 2014Wang C L. The functional identification of the trehalose-6-phosphate synthase 11 and three late embryogenesis abundant genes. Taian: Shandong Agricultural University, 2014
    [28] Li S, Yang Y, Zhang Q, Liu N, Xu Q, Hu L. Differential physiological and metabolic response to low tem-perature in two zoysiagrass genotypes native to high and low latitude. PLoS ONE, 2018, 13(6): e0198885
    [29] Caverzan A, Passaia G, Rosa S B, Ribeiro C W, Lazzarotto F, Margis-Pinheiro M. Plant responses to stresses: Role of ascorbate peroxidase in the antioxidant protection. Genetics and Molecular Biology, 2012, 35(4): 1011-1019
    [30] Song L X, Xu X C, Wang F N, Wang Y, Xia X J, Shi K, Zhou Y H, Zhou J, Yu J Q. Brassinosteroids act as a positive regulator for resistance against root-knot nematode involving respiratory burst oxidase homolog-dependent activation of MAPKs in tomato. Plant Cell and Environment, 2018 ,41(5):1113-1125
    [31] Liu A, Hu Z, Bi A, Fan J, Gitau M M, Amombo E, Chen L, Fu J. Photosynthesis, antioxidant system and gene expression of bermudagrass in response to low temperature and salt stress. Ecotoxicology, 2016, 25 (8): 1445-1457
    [32] 丁海东,朱晓红,刘慧, 张冬平,陈一,梁建生. ABA 信号转导途径中的MAPKS. 植物生理学报, 2011, 47(12): 1137-1144Ding H D, Zhu X H, Liu H, Zhang D P, Chen Y, Liang J S. Mitogen-activated protein kinases in abscisic acid signal transduction. Plant Physiology Journal, 2011, 47 (12): 1137-1144
    [33] 张庆怡,李悦,董佳伟,徐洪伟,周晓馥. 外源ABA对低温胁迫下牛皮杜鹃气孔性状及光合性能的影响. 北方园艺, 2022(1): 72-79Zhang Q Y, Li Y, Dong J W, Xu H W, Zhou X F. Effects of exogenous ABA on stomatal characters and photosynthetic characteristics of Rhododendron chrysanthemum Pall under low temperature stress. Northern Horticulture, 2022(1): 72-79
    [34] Nagao M, Minami A, Arakawa K, Fujikawa S, Takezawa D. Rapid degrada-tion of starch in chloroplasts and concomitant accumulation of soluble sugars associated with ABA-induced freezing tolerance in the moss Physcomitrella patens. Joumal of Plant Physiology, 2005, 162: 169-180
    [35] Hubbard K E, Nishimura N, Hitomi K, Getzoff E D, Schroeder J I. Early abscis-is acid signal transduction mechanisms: newly discov-ered components and newly emerging questions. Genes Development, 2010, 24: 1695-1708
    [36] Boneh U, Biton I, Schwartz A, Ben-Ari G. Characterization of the ABA signal transduction pathway in Vitis vinifera. Plant Science, 2012, 187: 89-96
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History
  • Received:August 11,2022
  • Revised:September 03,2022
  • Online: January 12,2023
  • Published: January 12,2023
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