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Home > Archive>Volume 24, Issue 2, 2023 >474-482. DOI:10.13430/j.cnki.jpgr.20221012002
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Genetic Effect of the Folate Content in F0-Generation Fresh Waxy Maize
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Affiliation:

Maize Research Institute of Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding， Beijing 100097

Fund Project:

Foundation projects： Youth Scientific Research Foundation of Beijing Academy of Agriculture and Forestry Sciences （QNJJ202208）； Special Program for Innovation of Beijing Academy of Agriculture and Forestry Sciences （KJCX201907-2）

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    Abstract:

    The bio-enhancement breeding for higher folate in food crops is a potential effective way to improve folate deficiency in humans. It is of great significance to study the genetic effects of folate traits in waxy maize F0 kernels. Two components 5-methyltetrahydrofolate （5-M-thf） and 5-formyltetrahydrofolate （5-F-thf） are the main forms of natural folate. In order to study the content and variants of folate in F0 kernels， twenty crosses were generated according to Griffing diallel cross using 5 waxy corn inbred lines， followed by the evaluation of the 5-M-thf and 5-F-thf contents of fresh F0 kernels in this study. The results showed that the content of 5-M-thf was higher than that of 5-F-thf in all crosses， and the content of 5-M-thf in ZN3/J6 was highest， which was 432.85 ug/100 g DW； the content of 5-F-thf in BN2/ZN3 was the highest， which was 115.64 ug/100 g DW. The F0 heterosis was observed in the 5-M-thf and 5-F-thf. The 5-F-thf showed positive heterosis in all crosses， while the 5-M-thf showed variants on heterosis in different crosses. Further calculation of the heritability and maternal effect of folate traits showed that the heritability of 5-F-thf （0.81） was higher than that of 5-M-thf， while the maternal effect of 5-M-thf （0.16） was higher than that of 5-F-thf. In conclusion， this study revealed the F0 heterosis and maternal effect of folate trait in waxy maize kernels， and the different performance on forms of folate in maize kernels. The inbred lines such as BN2 and ZN3， which were detected with high folate content， can be applied as female parent to obtain high folate content in maize F0 kernels.

    Reference
    [1] 江均平, 孙艳丽, 裴志超, 王艳, 张丽静, 周继华, 孟范玉, 佟国湘, 王占森, 张旭, 王凤忠. 北京鲜食甜玉米营养成分分析与评价. 中国食物与营养,2020, 26 (8): 55-59Jiang J P, Sun Y L, Pei Z C, Wang Y, Zhang L J, Zhou J H, Meng F Y, Tong G X, Wang Z S, Zhang X, Wang F Z. Analysis and evaluation on nutritional components of fresh sweet corn in Beijing city. Food and Nutrition in China, 2020, 26 (8): 55-59
    [2] 杨若明, 李玉田. 玉米鲜食的功效和鲜食玉米的研究开发. 北京农业科学, 1997,15 (5): 41-43Yang R M, Li Y T. The efficacy of fresh corn and the research and development of fresh corn. Beijing Agricultural Sciences, 1997,15 (5): 41-43
    [3] 李玉军, 刘婷婷, 张泽志. 糯玉米起源、研究及发展概况. 耕作与栽培, 2010 (3): 52-53Li Y J, Liu T T, Zhang Z Z. The origin, research and development of waxy corn. Tillage and Cultivation, 2010 (3): 52-53
    [4] 王义发, 汪黎明, 沈雪芳, 张璧, 郑洪建. 糯玉米的起源、分类、品种改良及产业发展. 湖南农业大学学报:自然科学版, 2007, 33 (10): 97-102Wang Y F, Wang L M, Shen X F, Zhang B, Zheng H J. Origin, classification, variety improvement and industry development of waxy corn. Journal of Hunan Agricultural University: Natural Sciences Edition, 2007, 33 (10): 97-102
    [5] 黄小兰, 孙权星, 姜健, 彭长俊, 陈小晖, 顾志华. 糯玉米的应用与开发. 现代农业科技, 2012 (2): 85-86Huang X L, Sun Q X, Jiang J, Peng C J, Chen X H, Gu Z H. Application and development of waxy corn. Modern Agricultural Science and Technology, 2012 (2): 85-86
    [6] 卢柏山, 徐丽, 赵久然, 史亚兴, 樊艳丽, 席胜利, 张翠芬, 史亚民, 张小继. 京科糯2000等系列鲜食糯玉米品种选育及应用. 玉米科学, 2019, 27 (5): 1-4Lu B S, Xu L, Zhao J R, Shi Y X, Fan Y L, Xi S L, Zhang C F, Shi Y M, Zhang X J. Breeding and application of Jingkenuo2000 series of waxy corn varieties. Journal of Maize Sciences, 2019, 27 (5): 1-4
    [7] 赵久然, 卢柏山, 史亚兴, 徐丽. 我国糯玉米育种及产业发展动态. 玉米科学, 2016, 24 (4): 67-71Zhao J R, Lu B S, Shi Y X, Xu L. Development trends of waxy corn breeding and industry in China. Journal of Maize Sciences, 2016, 24 (4): 67-71
    [8] Xiu Y, Field M S. The roles of mitochondrial folate metabolism in supporting mitochondrial DNA synthesis, oxidative phosphorylation, and cellular function. Current Developments in Nutrition, 2020, 4 (10): a153
    [9] 顾益银, 韩莹琰. 植物体内叶酸代谢及生物强化研究进展. 分子植物育种, 2021,URL:http://kns.cnki.net/kcms/detail/46.1068.S.20210629.1137.004.htmlGu Y Y, Han Y Y. Research progress in folate metabolism and bio-enhancement of plants. Molecular Plant Breeding, 2021,URL:http://kns.?cnki.?net/kcms/detail/46.1068.?S.?20210629.1137.004.html
    [10] 康文怀, 叶晓利, 李慧, 李巧玲, 秦玲. 食品中叶酸分析方法及稳定性研究进展. 河北科技大学学报, 2019, 40 (5): 446-453Kang W H, Ye X L, Li H, Li Q L, Qin L. Advances in research on analysis methods and stability of folic acid in food. Journal of Hebei University of Science and Technology, 2019, 40 (5): 446-453
    [11] 王博伦. 鲜糯玉米制品加工过程中叶酸稳定性研究. 北京: 中国农业科学院, 2020Wang B L. Study on the stability of folate during processing of fresh waxy maize products. Beijing:? ?Chinese Academy of Agricultural Sciences, 2020
    [12] Moat S J, Lang D, McDowell I F W, Clarke Z L, Madhavan A K, Lewis M J, Folate Goodfellow J., homocysteine, endothelial function and cardiovascular disease. The Journal of Nutritional Biochemistry, 2004, 15 (2): 64-79
    [13] Snowdon D A, Tully C L, Smith C D, Riley K P, Markesbery W R. Serum folate and the severity of atrophy of the neocortex in Alzheimer disease: Findings from the nun study. The American Journal of Clinical Nutrition, 2000, 71 (4)?: 993-998
    [14] Kolb A F, Petrie L. Folate deficiency enhances the inflammatory response of macrophages. Molecular Immunology, 2013, 54 (2): 164-172
    [15] Gough K R, Read A E, Mccarthy C F, Waters A H. Megaloblastic anaemia due to nutritional deficiency of folic acid. The Quarterly Journal of Medicine, 1963, 32 (3): 243-256
    [16] Arth A, Kancherla V, Pachón H, Zimmerman S, Johnson Q, Jr G P O. A 2015 global update on folic acid-preventable spina bifida and anencephaly. Birth defects research. Part A, Clinical and Molecular Teratology, 2016, 106 (7): 520-529
    [17] Liew S. Folic acid and diseases - supplement it or not？ Brazil: Associa??o Médica Brasileira, 2016, 62 (1): 90-100
    [18] Cho E, Zhang X, Townsend M K, Selhub J, Paul L, Rosner B, Fuchs C S, Willett W C, Giovannucci E L. Unmetabolized folic acid in prediagnostic plasma and the risk of colorectal cancer. Journal of the National Cancer Institute, 2015, 107 (12): v260
    [19] 苏新宏, 夏宗良, 李潮海. 玉米杂交当代优势研究进展. 玉米科学, 2008 (1): 44-46Su X H, Xia Z L, Li C H. Advances in studies on heterosis of F0 generation in maize. Journal of Maize Sciences, 2008 (1): 44-46
    [20] 洪俊彦, 黄仁, 黄春颖, 王建华, 徐一帆, 李佩佩, 胡渊渊, 黄坚钦, 李岩. 植物花粉直感的研究进展及展望. 植物生理学报, 2020, 56 (2): 151-162Hong J Y, Huang R, Huang C Y, Wang J H, Xu Y F, Li P P, Hu Y Y, Huang J Q, Li Y. Research progress and prospects of xenia. Plant Physiology Journal, 2020, 56 (2): 151-162
    [21] 朱焕焕, 靳颖玲, 张明科, 张鲁刚, 惠麦侠. 大白菜花粉直感效应研究. 华北农学报, 2016, 31 (3): 107-113Zhu H H, Qin Y L, Zhang M K, Zhang L G, Hui M X. Studies on xenia effect of Chinese cabbage. Acta Agriculturae Boreali-Sinica, 2016, 31 (3): 107-113
    [22] 季良越, 胡彦民, 李永亮, 黄素香, 季洪强. 玉米雄性不育单交种再杂交当代杂种优势效应研究. 中国农业科学, 2002, 35 (3): 250-255Ji Y L, Hu Y M, Li Y L, Huang S X, Ji H Q. Study on heterosis effect of re-crossed on male-sterile single-hybrids of corn. Scientia Agricultura Sinica, 2002, 35 (3): 250-255
    [23] Bulant C, Gallais A. Xenia effects in maize with normal endosperm. I. Importance and stability. Crop Sciences, 1998, 38 (6): 1517-1525
    [24] 李依依, 田汉钊, 朱良佳, 刘美, 赵永锋. 玉米粒重杂交当代杂种优势分析. 种子, 2020, 39 (4): 22-28Li Y Y, Tian H Z, Zhu L J, Liu M, Zhao Y F. Heterosis analysis within contemporary maize based on hybridization of grain weight. Seed, 2020, 39 (4): 22-28
    [25] 李浩川, 刘宗华, 王琳, 汤继华, 胡彦民, 谭晓军. 杂交当代玉米子粒蛋白质含量的正反交效应及杂种优势分析. 河南农业大学学报, 2007, 41 (1): 1-4Li H C, Liu Z H, Wang L, Tang J H, Hu Y M, Tan X J. Analysis on the effect of reciprocal crosses and heterosis about protein content of F1 seeds in maize grain. Journal of Henan Agricultural University, 2007, 41 (1): 1-4
    [26] 朱保侠, 裴玉贺, 郭新梅, 张恩盈, 刘兰浩, 宋希云. 花粉直感对普通玉米子粒淀粉含量的影响. 玉米科学, 2009, 17 (1): 89-91Zhu B X, Pei Y H, Guo X M, Zhang E Y, Liu L H, Song X Y. The effect of xenia on the starch of common maize kernel. Journal of Maize Sciences, 2009, 17 (1): 89-91
    [27] 宋同明, 吴宏平, 戴顺宏, 吴秀琴. 玉米含油量基因对高油与低油玉米互交当代子粒的遗传效应. 北京农业大学学报, 1991, 17 (1): 15-22Song T M, Wu H P, Dai S H, Wu X Q. Genetic effects of corn kernel oil controlling genes in reciprocal crosses. ACTA Agriculturae Universitatis Pekinensis,1991, 17 (1): 15-22
    [28] 董会, 徐丽, 史亚兴, 赵久然, 卢柏山, 樊艳丽, 席胜利, 张翠芬, 高宁, 史亚民, 刘焱辉. 糯玉米鲜子粒和熟子粒叶酸含量分析及评价. 玉米科学, 2019, 27 (2): 16-20Dong H, Xu L, Shi Y X, Zhao J R, Lu B S, Fan Y L, Xi S L, Zhang C F, Gao N, Shi Y M, Liu Y H. Analysis of folic acid content in fresh and cooked grains of waxy corn. Journal of Maize Sciences, 2019, 27 (2): 16-20
    [29] 孙振, 莫乔程, 程备久, 谢传晓, 林凤. 玉米雄穗分枝数性状遗传、杂种优势与亲子相关分析. 作物杂志, 2012 (2): 31-35Sun Z, Mo Q C, Cheng B J, Xie C X, Lin F. Analysis of inheritance, heterosis and parent-off spring correlation of tassel branch number in maize (Zea mays L.). Crops, 2012 (2): 31-35
    [30] 王通强. 作物母体效应计算方法探讨. 西南农业学报, 1997 (3): 26-29Wang T Q. Discussion on crop maternal effect estimating method. Southwest China Journal of Agricultural Sciences, 1997 (3): 26-29
    [31] Czarnowska-Kujawska M, Gujska E, Michalak J. Testing of different extraction procedures for folate HPLC determination in fresh fruits and vegetables. Journal of Food Composition and Analysis, 2017, 57: 64-72
    [32] Shin Y S, Kim E S, Watson J E, Stokstad E L. Studies of folic acid compounds in nature. IV. Folic acid compounds in soybeans and cow milk. Canadian Journal of Biochemistry, 1975, 53 (3): 338-343
    [33] Storozhenko S, De Brouwer V, Volckaert M, Navarrete O, Blancquaert D, Zhang G, Lambert W, Van Der Straeten D. Folate fortification of rice by metabolic engineering. Nature Biotechnology, 2007, 25 (11): 1277-1279
    [34] Lian T, Wang X, Li S, Jiang H, Zhang C, Wang H, Jiang L. Comparative transcriptome analysis reveals mechanisms of folate accumulation in maize grains. International Journal of Molecular Sciences, 2022, 23 (3): 1708
    [35] Lian T, Guo W, Chen M, Li J, Liang Q, Liu F, Meng H, Xu B, Chen J, Zhang C, Jiang L. Genome-wide identification and transcriptional analysis of folate metabolism-related genes in maize kernels. BMC Plant Biology, 2015, 15 (1):204
    [36] 李亚楠, Aye N C, 冯禹然, 董远, 师亚琴, 王博新, 徐淑兔, 薛吉全. 不同来源玉米自交系及杂交组合杂交当代子粒性状的差异分析. 作物杂志, 2016 (1): 23-27Li Y N, Aye N C, Feng Y R, Dong Y, Shi Y Q, Wang B X, Xu S T, Xue J Q. Differential analysis of kernel traits for maize inbred lines and hybrids from different sources. Crops, 2016 (1): 23-27
    [37] 郭文柱. 成熟玉米籽粒中5-甲酰四氢叶酸代谢关键基因的发掘及功能分析. 武汉:华中农业大学, 2019Guo W Z. Identification and functional analysis of key gene related to mature kernels 5-formyltetrahydrofolate in maize. Wuhan: Huazhong Agricultural University, 2019
    [38] Guo W Z, Lian T, Wang B, Guan J, Yuan D, Wang H, Safiul Azam F M, Wan X, Wang W, Liang Q, Wang H, Tu J, Zhang C, Jiang L. Genetic mapping of folate QTLs using a segregated population in maize. Journal Integrated Plant Biology, 2019, 61 (6): 675-690
    [39] Xiao Y, Yu Y, Xie L, Li K, Guo X, Li G, Liu J, Li G, Hu J. A genome-wide association study of folates in sweet corn kernels. Frontiers in Plant Science, 2022,13:1-11
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History
  • Received:October 12,2022
  • Revised:November 06,2022
  • Online: March 14,2023
  • Published: March 14,2023
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