2025-4-9- 23
Home > Archive>Volume 24, Issue 4, 2023 >944-953. DOI:10.13430/j.cnki.jpgr.20221213003
PDF HTML XML Export Cite reminder
Characterization of Different 2P Wheat-Agropyron cristatum Addition Lines
Author:
Affiliation:

Institute of Crop Sciences， Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement， Beijing 100081

Fund Project:

Foundation project： The National Natural Science Foundation of China（32272083）

  • Article
    • | |
  • Metrics
    • |
  • Reference [45]
    • | | | |
  • Comments
    Abstract:

    Agropyron cristatum， as one of the important wild relatives of wheat， contains many excellent genes that are useful in wheat improvement. The wheat-A. cristatum addition line， which contains a complete A. cristatum chromosome， serves as an important bridge to utilize the A. cristatum derived excellent genes. As a perennial tetraploid outcrossing species that carries four haploid sub-genomes， the wheat-A. cristatum additional lines carrying each of different chromosomes from the same homologous group might represent the different agronomic characters. In this study， the heading time， plant type， panicle character， grain character， resistance to powdery mildew and leaf rust were identified and analyzed employing cytological identification， molecular marker detection， agronomic character investigation and disease resistance identification. The wheat-A. cristatum 2P addition line II-9-3 was genetically stable， compact， immune to leaf rust and highly resistant to powdery mildew， which could be used for plant architecture improvement and disease resistance breeding. Wheat-A. cristatum 2P addition line II-3-1b was observed with obvious reduction on plant height， early heading and immune to leaf rust， which could be used for plant height improvement and leaf rust resistance breeding. Wheat-A. cristatum 2P addition line II-23-72 is nearly immune to endemic leaf rust and powdery mildew species， and might be useful in disease-resistance breeding. These three 2P addition lines provided raw materials for further producing 2P translocation and deletion lines. These 2P addition lines with different characteristics on plant type， plant height and disease resistance， might provide a basis for future genetic mapping and effective use of excellent genes applicable in wheat.

    Reference
    [1] 张洁, 蒋云, 郭元林, 王颖, 邓光兵, 宣朴, 龙海. 普通小麦川麦60-簇毛麦3V(3D)代换系的分子细胞学鉴定. 麦类作物学报, 2022, 42(2): 139-146Zhang J, Jiang Y, Guo Y L, Wang Y, Deng G B, Xuan P, Long H. Molecelar and cytological identification of common wheat Chuanmai 60-Dasypyrumvillosum 3V(3D) substitution line. Journal of Triticeae Crops, 2022, 42(2): 139-146
    [2] Bai S S, Zhang H B, Han J, Wu J H, Li J C, Geng X X, Lü B Y, Xie S F, Han D J, Zhao J X, Yang Q H, Wu J, Chen X H. Identification of genetic locus with resistance to take-all in the wheat-Psathyrostachys huashanica Keng introgression line H148. Journal of Integrative Agriculture, 2021, 20(12): 3101-3113
    [3] 李立会, 杨欣明, 李秀全, 董玉琛. 中国小麦野生近缘植物的研究与利用. 中国农业科技导报, 2000(6): 73-76Li L H, Yang X M, Li X Q, Dong Y C. Study and utilization of wild relatives of wheat in China. China Agricultural Science and Technology Review, 2000(6): 73-76
    [4] 孙洋洋, 陈红新, 刘伟华, 韩海明, 周升辉, 杨欣明, 李秀全, 张锦鹏, 李立会. 小麦—冰草T7PL·7AL罗伯逊易位系的分子细胞学鉴定. 植物遗传资源学报, 2018, 19(6): 1038-1044Sun Y Y, Chen H X, Liu W H, Han H M, Zhou S H, Yang X M, Li X Q, Zhang J P, Li L H. Cytological identification of wheat-A.cristatum T7PL. 7AL Robertsonian translocation Line. Journal of Plant Genetic Resources, 2018, 19(6): 1038-1044
    [5] Ochoa V, Madrid E, Said M, Rubiales A, Cabrere A. Molecular and cytogenetic characterization of a common wheat-Agropyron cristatum chromosome translocation conferring resistance to leaf rust. Euphytica, 2015,201: 89-95
    [6] Ezatollah F, Rahmani S, Jowkar M, Shabani A. Estimation of genetic parameters and chromosomal localization of QTLs controlling agro-physiological indicators of drought tolerance in agropyron using wheat-agropyron disomic addition lines. Australian Journal of Crop Science, 2014, 8: 133-139
    [7] 韩冉, 刁瑞宁, 梁海凡, 訾妍, 汪晓璐. 小麦遗传改良中的重要基因源——冰草. 山东农业科学, 2019, 51(8): 1-9Han R, Diao R N, Liang H F, Xi Y, Wang X L. Important gene source for wheat genetic improvement-Agropyron cristatum. Shandong Agricultural Sciences, 2019, 51(8): 1-9
    [8] 王峰, 丁云花, 李成琼, 简元才. 植物异源附加系研究进展. 北方园艺, 2012 (19):195-199Wang F, Ding Y H, Li C Q, Jian Y C. Research progress in alien plant additional lines. Northern Horticulture, 2012 (19):195-199
    [9] 陈士强, 黄泽峰, 张勇, 葛江燕, 朱雪, 高勇, 陈建民. 中国春背景下长穗偃麦草抗赤霉病相关基因的染色体定位. 麦类作物学报, 2012, 32(5): 839-845Chen S Q, Huang Z F, Zhang Y, Ge J Y, Zhu X, Gao Y, Chen J M. Chromosomal location of the genes associated with FHB resistance of Lophopyrum elongatum in Chinese Spring background. Journal of Triticeae Crops, 2012, 32(5): 839-845
    [10] 赵继新, 武军, 程雪妮, 董剑, 陈新宏, 刘淑会, 杜万里, 庞玉辉, 杨群慧, 吉万全, 傅杰. 普通小麦-华山新麦草1Ns二体异附加系的农艺性状和品质. 作物学报, 2010, 36(9): 1610-1614Zhao J X, Wu J, Cheng X N, Dong J, Chen X H, Liu S H, Du W L, Pang Y H, Yang Q H, Ji W Q, Fu J. Agronomic and quality traits of a wheat-Psathyrostachys huashanica 1Ns disomic addition line. Acta Agrinomica Sinica, 2010, 36(9): 1610-1614
    [11] Yang W J, Wang C Y, Chen C H, Wang Y J, Zhang H, Liu X L, Ji W Q. Molecular cytogenetic identification of a wheat-rye 1R addition line with multiple spikelets and resistance to powdery mildew. Genome, 2016,59: 277-288
    [12] 郑瑗, 李欣, 贾举庆, 郭慧娟, 雷梦林, 张树伟, 常利芳, 畅志坚, 乔麟轶, 陈芳, 张晓军. 抗条锈病小偃麦附加系CH366的FISH鉴定. 山西农业科学, 2021, 49(3): 278-280,284Zheng Y, Li X, Jia J Q, Guo H J, Lei M L, Zhang S W, Chang L F, Chang Z J, Qiao L Y, Chen F, Zhang X J. FISH identification of wheat-Thinopyrum intermedium addition line CH366 with resistance to stripe rust. Journal of Shanxi Agricultural Sciences, 2021, 49(3): 278-280,284
    [13] 宋全昊, 金艳, 宋佳静, 陈杰, 赵立尚, 白冬, 陈亮, 朱统泉. 61份小麦异源附加系种质的表型多样性分析. 江苏农业科学, 2022, 50(16): 79-86Song Q H, Jin Y, Song J J, Chen J, Zhao L S, Bai D, Chen L, Zhu T Q. Phenotypic diversity analysis of 61 wheat addition lines germplasm resources. Jiangsu Agricultural Sciences, 2022, 50(16): 79-86
    [14] Han H M, Liu W H, Zhang J P, Zhou S H, Yang X M, Li X Q, Li L H. Identification of P genome chromosomes in Agropyron cristatum and wheat-A. cristatum derivative lines by FISH. Scientific Reports, 2019,9: 9712
    [15] Luan Y, Wang X G, Liu W H, Li C Y, Zhang J P, Gao A N, Wang Y D, Yang X M, Li L H. Production and identification of wheat-Agropyron cristatum 6P translocation lines. Planta, 2010,232:501-510
    [16] Han H M, Li B, Su J J, Zhang J P, Song L Q, Gao A N, Yang X M, Li X Q, Liu W H, Li L H. Genetic rearrangements of six wheat-Agropyron cristatum 6P addition lines revealed by molecular markers. Public Library of Science, 2014, 9: e91066
    [17] Li Q F, Lu Y Q, Pan C L, Zhang J P, Liu W H, Yang X M, Li X Q, Xi Y J, Li L H. Characterization of a novel wheat-Agropyron cristatum 2P disomic addition line with powdery mildew resistance. Crop Science, 2016,56: 2390-2400
    [18] Chen H X, Han H M, Li Q F, Zhang J P, Lu Y Q, Yang X M, Li X Q, Liu W H, Li L H. Identification and genetic analysis of multiple P chromosomes of Agropyron cristatum in the background of common wheat. Journal of Integrative Agriculture, 2018,17: 1697-1705
    [19] Pan C L, Li Q F, Lu Y Q, Zhang J P, Yang X M, Li X Q, Li L H, Liu W H. Chromosomal localization of genes conferring desirable agronomic traits from Agropyron cristatum chromosome 1P. Public Library of Science, 2017,12: e0175265
    [20] Li H H, Lv M J, Song L Q, Zhang J P, Gao A N, Li L H, Liu W H. Production and identification of wheat-Agropyron cristatum 2P translocation lines. Public Library of Science, 2016,11: e0145928
    [21] Li H H, Jiang B, Wang J C, Lu Y Q, Zhang J P, Pan C L, Yang X M, Li X Q, Liu W H, Li L H. Mapping of novel powdery mildew resistance gene(s) from Agropyron cristatum chromosome 2P. Theoretical and Applied Genetics, 2017,130: 109-121
    [22] 李荣华, 夏岩石, 刘顺枝, 孙莉丽, 郭培国, 缪绅裕, 陈健辉. 改进的CTAB提取植物DNA方法. 实验室研究与探索, 2009, 28(9): 14-16Li R H, Yan Y S, Liu S X, Sun L L, Guo P G, Miao S Y, Chen J H. CTAB-improved method of DNA extraction in plant. Laboratory Research and Exploration, 2009, 28(9): 14-16
    [23] Zhang J P, Liu W H, Lu Y Q, Yang X M, Li X Q, Li L H. A resource of large-scale molecular markers for monitoring Agropyron cristatum chromatin introgression in wheat background based on transcriptome sequences. Scientific Reports, 2017,7: 11942
    [24] 李立会,李秀全,杨欣明. 小麦种质资源描述规范和数据标准. 北京: 中国农业出版社, 2006: 2-3Li L H, Li X Q, Yang X M. Descriptive specifications and data standards for wheat germplasm resources. Beijing: China Agriculture Press, 2006: 2-3
    [25] Zhao D H, Li Yang, Xu K J, Cao S H, Tian Y B, Yan J, He Z H, Xia X C, Song X Y, Zhang Y. Identification and validation of genetic loci for tiller angle in bread wheat. Theoretical and Applied Genetics, 2020,133: 3037-3047
    [26] 马超, 董振杰, 田修斌, 王贝麟, 张玥琦, 李欢欢, 刘文轩. 来自西尔斯山羊草的抗小麦白粉病基因Pm57抗性丧失突变体的筛选与鉴定. 植物遗传资源学报, 2020, 21(2): 386-393Ma C, Dong Z J, Tian X B, Wang B L, Zhang Y Q, Li H H, Liu W X. Screening and identification of Pm57 mutants with loss of resistance to powdery mildew derived from Aegilops searsii. Journal of Plant Genetic Resources, 2020, 21(2): 386-393
    [27] 盛宝钦. 用反应型记载小麦苗期白粉病. 植物保护, 1988(1): 49Sheng B Q. Record wheat powdery mildew at seedling stage by reactive type. Plant protection, 1988(1): 49
    [28] Liu T G, Chen W Q. Race and virulence dynamics of Puccinia triticina in China during 2000-2006. Plant Disease, 2012, 96(11): 1601-1607
    [29] Roefls A P. Race specificity and methods of study. The Cereal Rust, 1985,1: 131-164
    [30] 张德时, 王斯文, 王长有, 王艳珍, 陈春环, 吉万全, 张宏. 小麦-华山新麦草异附加系的细胞遗传学和分子标记辅助鉴定.麦类作物学报, 2020, 40(1): 12-20Zhang D S, Wang S W, Wang C Y, Wang Y Z, Chen C H, Ji W Q, Zhang H. Cytogenetics and marker assisted identification of wheat-Psathyrostachys huashanica alien addition lines. Journal of Triticeae Crops, 2020, 40(1): 12-20
    [31] 程舒唯, 王惠, 赵心宇, 于晓宁, 高岐玉, 宋维富, 杨雪峰, 宋庆杰, 丁佳蕊, 张会新, 张延明. 小偃麦的创制及应用研究进展. 植物遗传资源学报, 2023, 24(1): 86-101Chen S W, Wang H, Zhao X Y, Yu X N, Gao Q Y, Song W F, Yang X F, Song Q J, Ding J R, Zhang H X, Zhang Y M. Progress on the development and application of trititrigia in wheat. Journal of Plant Genetic Resources, 2023, 24(1): 86-101
    [32] 吕博雅, 张珍悦, 赵李, 李晓萍, 李家创, 白宇皓, 刁慧珊, 刘洋, 杨群慧, 刘淑会, 武军, 陈新宏. 华山新麦草2Ns染色体SCAR标记的开发. 麦类作物学报, 2022, 42(5): 539-545Lü B Y, Zhang Z Y, Zhao L, Li X P, Li J C, Bai Y H, Diao H S, Liu Y, Yang Q H, Liu S H, Wu J, Chen X H. Development of a new SCAR marker on chromosome 2Ns of Psathyrostachys huashanica keng. Journal of Triticeae Crops, 2022, 42(5): 539-545
    [33] 于军伟, 王斯文, 姚广平, 赵继新, 陈春环, 吉万全, 王长有. 抗条锈病普通小麦-中间偃麦草7St二体异附加系的分子细胞遗传学鉴定. 麦类作物学报, 2021, 41(10): 1189-1196Yu J W, Wang S W, Yao G P, Zhao J X, Chen C H, Ji W Q, Wang C Y. Molecular cytogenetic identification of wheat-Thinopyrum intermedium 7St disomic alien addition line conferring stripe rust resistance. Journal of Triticeae Crops, 2021, 41(10): 1189-1196
    [34] 李立会, 董玉琛. 冰草属研究进展. 遗传, 1993, 15(1): 45-48Li L H, Dong Y C. Progress in studies of Agropyron Gaertn. Genetic, 1993, 15(1): 45-48
    [35] 刘成, 韩冉, 汪晓璐, 宫文萍, 程敦公, 曹新有, 刘爱峰, 李豪圣, 刘建军. 小麦远缘杂交现状、抗病基因转移及利用研究进展. 中国农业科学, 2020, 53(7): 1287-1308Liu C, Han R, Wang X L, Gong W P, Cheng D G, Cao X Y, Liu A F, Li H S, Liu J J. Research progress of wheat wild hybridization, disease resistance genes transfer and utilization. Scientia Agricultural Sinica, 2020, 53(7): 1287-1308
    [36] 杨兆生, 许红霞, 梁文科. 小麦超高产紧凑型新品种的选育. 麦类作物学报, 1996, 16(2): 17-18Yang Z S, Xu H X, Liang W K. Selection and breeding of new compact wheat varieties with super high yield. Journal of Triticeae Crops, 1996, 16(2): 17-18
    [37] Kowalski A M, Gooding M, Ferrante A, Slafer S, Orford S, Gasperini D, Griffiths S. Agronomic assessment of the wheat semi-dwarfing gene Rht8 in contrasting nitrogen treatments and water regimes. Field Crops Research, 2016,191: 150-160
    [38] Divashuk M G, Vasil′ev A V, Bespalova L A, Karlov G I. Identity of the Rht-11 and Rht-B1e reduced plant height genes. Russian Journal of Genetics, 2012, 48(7): 761-763
    [39] Goudia B D, Liang C, Hu Y G. Agronomic traits affected by dwarfing gene Rht-5 in common wheat (Triticum aestivum L.). Australian Journal of Crop Science, 2013, 7(9): 1270-1276
    [40] 刘畅, 李仕金, 王轲, 叶兴国, 林志珊. 簇毛麦6VS特异转录序列P21461及P33259的获得及其分子标记在鉴定小麦-簇毛麦抗白粉病育种材料中的应用. 作物学报, 2017, 43(7): 983-992Liu C, Li S J, Wang K, Ye X G,Lin Z S. Developing of specific transcription squences P21461 and P33259 on dasypyrum villosum 6VS and application of molecular markers in identifying wheat-D.villosum breeding materials with powdery mildew resistance. Acta Agrinomica Sinica, 2017, 43(7): 983-992
    [41] Fang Z W, Sun C, Lu T, Xu Z, Huang W D, Ma D F, Yin J L. Molecular mapping of stripe rust resistance gene YrH922 in a derivative of wheat(Triticum aestivum)-Psathyrostachys huashanica. Crop and Pasture Science, 2019, 70(11): 939-945
    [42] 闫红飞, 杨文香, 褚栋, 刘大群. 小麦抗叶锈基因Lr38的一个新标记. 中国农业科学, 2008(11): 3604-3609Yan H F, Yang W X, Chu D, Liu D Q. A New marker tagged to the leaf rust resistance gene Lr38. Scientia Agricultura Sinica, 2008(11): 3604-3609
    [43] 魏学军, 张海涛, 张娜, 胡亚亚, 谢欢, 赵丽娟, 杨文香. 粗山羊草苗期抗叶锈性离体鉴定及抗叶锈基因推导. 麦类作物学报, 2013, 33(5): 907-911Wei X J, Zhang H T, Zhang N, Hu Y Y,Xin H, Zhao L J, Yang W X. Surveying resistance to wheat leaf rust and postulation of resistant genes by detached leaves in Aegilops tauschii. Journal of Triticeae Crops, 2013, 33(5): 907-911
    [44] 王佳荣, 董瑞, 张梦宇, 高璞, 张培培, 李在峰, 刘大群. 40份CIMMYT小麦品系苗期及成株抗叶锈病基因鉴定.华北农学报, 2022, 37(2): 201-210Wang J R, Dong R, Zhang M Y, Gao P, Zhang P P, Li Z F, Liu D Q. Identification of leaf rust resistance genes in 40 CIMMYT wheat lines at seeding stage and adult plant. Acta Agriculturae Boreali-sinica, 2022, 37(2): 201-210
    [45] 赵佳佳, 刘鑫, 王超, 杨武德. 小麦抗叶锈病新种质的创制与鉴定. 山西农业科学, 2022, 50(9): 1221-1228Zhao J J, Liu X, Wang C, Yang W D. Creation and identification of leaf rust resistance new germplasm of wheat. Journal of Shanxi Agricultural Sciences, 2022, 50(9): 1221-1228
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

Copy
Share
Article Metrics
  • Abstract:359
  • PDF: 2362
  • HTML: 259
  • Cited by: 0
History
  • Received:December 13,2022
  • Revised:January 06,2023
  • Online: June 13,2023
  • Published: June 14,2023
Article QR Code
You are the 609287th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.