Abstract:This study attempted to evaluate the plant height, grain traits, and diseases resistance of wheat resources derived from different wheat regions of China at four major breeding stages that were cultivated in spring wheat region of Ningxia Hui Autonomous region, China, in order to provide the methods and excellent germplasms for local wheat breeding in future. A total of 228 wheat resources at four breeding stages were used. The entropy-weighted DTOPSIS method was used for evaluating the plant height and grain traits in order to select good breeding parents. The diseases resistance and proportion of resistant genes were evaluated by field resistance survey, in combination with 18 molecular markers that associate with main wheat diseases in Ningxia, China. The results showed that, at the breeding stages I to IV, the plant height and index of the protein traits were gradually decreased, while the grain number and grain weight were gradually increased. According to the Ci value, 11 resources (Hongxinglong No.3, Nuomai, Xinjiang wheat, Shanglin wheat, Ningchun No.45, Huining No.19, Ningchun No.32, Ningchun No.58, Ganchun No.25, Yongliang No.15 and Xiaobing No.33) ranking top 5% in popupalation were identified. The resistance survey results showed, there were 181, 220 and 83 in 228 resources that were resistant to stripe rust, leaf rust and powdery mildew, respectively. These 11 resources based on the Ci value showed better resistance to stripe rust and leaf rust, and three of them, namely Hongxinglong No.3, Shanglin wheat, and Xiaobing No. 33, were also resistant to powdery mildew. In addition, 18 molecular markers associated with diseases resistance, showed different proportions of amplification results in 228 resources. Collectively, the DTOPSIS method based on entropy weighting and molecular markers associated with main wheat diseases can be used to evaluate the wheat resources at the different breeding stages.These selected wheat resources with good performance can be used as candidate parents for wheat breeding in Ningxia, China.