In order to analyze leaf rust resistance genes present in 40 domestic wheat cultivars， using 16 physiological races of leaf rust fungus to deduce their genes， it is speculated that the composition of the resistance genes to leaf rust disease in the tested material. And combined with 11 specific molecular markers closely linked to known disease resistance genes for validation. And further select 5 highly virulent races to make mixed strains， and conduct plant adult inoculation in the Baoding experimental field to screen wheat varieties that may contain adult slow rust genes. Based on pedigree analysis， gene postulation and molecular marker detection results， six known major leaf rust resistance genes （Lr1， Lr11， Lr20， Lr26， Lr30 and Lr37） were detected. Ten varieties including Yunhei 14207 contain Lr1； Hemei 988 and Bainong 207 contain Lr11； Yumai 49 and Bainong 207 contain Lr20； 23 varieties including Wanfeng 269 contain Lr26； Yunhei 14207 and Zhengmai 103 contain Lr30； four varieties including Luo6073 contain Lr37， and some varieties contain unknown genes for leaf rust resistance. Seven varieties， such as Luo 6073， were identified showing slow rust phenotype. Collectively， this study clarified the genetic composition of these varieties， and obtained elite resistant varieties which can be applied in resistance breeding and resistance gene stacking.