Functional rice with high resistant starch content improves human health considering the risk of diet-related chronic diseases. Exploring the resistant starch-related genes is of great significance to reveal the genetic mechanism of rice grain resistant starch synthesis， which has implication in breeding rice varieties with high resistant starch. In this study， 126 F₇ recombinant inbred lines （RILs） from cross between Ningxia local high-yield japonica rice variety Ningjing 28 and imported japonica rice variety JTD were used. A genetic linkage map containing 1，856 Bin markers was constructed by whole genome resequencing technology， showing a length of 1973.86 cM， and the average genetic distance of 1.06 cM between markers. Using CIM interval mapping method to map the QTL of resistant starch traits in rice populations， one QTL controlling resistant starch was detected on chromosome 7， with a LOD value of 4.2 and a contribution rate of 9.7%. Through in-depth analysis， two candidate genes Os07g0444000 （predicted as rice glucosylceramidase） and Os07g0443500 （DNA binding protein containing MYB domain） were obtained. The study found that the genotype frequencies of SNP_15740179 and SNP_15740207 on Os07g0444400 were significantly different between the high resistant starch group and the low resistant starch group in the RIL population. During the grain filling process， the expression levels of Os07g0443500 and Os07g0444000 in the male parent JTD（high resistant starch parent） were higher than those of the female parent Ningjing 28. These results implied that both candidate genes may be involved in forming resistant starch during rice grain filling. Collectively， this study provides new genetic resources for the breeding of new rice varieties with high resistant starch by mining the QTL loci and related genes of rice grain-resistant starch.