C2H2 zinc finger protein transcription factor plays an important role in plant growth and development， stress response and regulation of secondary metabolism synthesis. The previous study found that the flavonoid content in the root of fagopybuckwheat was higher than that of Tartary buckwheat， which may be due to the amplification of gene families related to flavonoid biosynthesis， such as FdCHI， FdF3H and FdDFR. However， it has not been reported how the C2H2 zinc finger protein transcription factor family genes involved in flavonoid biosynthesis regulate rutin synthesis in golden buckwheat. In this study， the whole genome of FdC2H2-ZFP transcription factor was identified and its expression profile was analyzed. A total of 114 FdC2H2-ZFPs were identified. Based on the analysis of RNA-Seq data， the C2H2 zinc finger protein gene FdC2H2-2 was screened and cloned. This gene has three typical C2H2 zinc finger structures， and has high homology with Arabidopsis AtTREE1 and AtDAZ3. qRT-PCR showed that the expression of FdC2H2-2 gene was significantly induced by jasmonic acid. In addition， the content of rutin in the hairy roots of overexpressing FdC2H2-2 was significantly higher than that of the control， and the transcriptional expression of flavonol synthetase （FLS）， phenylalanine aminolyase （PAL） and flavonoid 3'，5'-hydroxylase （F3'5'H）， the key enzyme genes in the rutin synthesis pathway were significantly increased. These results suggest that FdC2H2-2 gene may positively regulate the accumulation of rutin by activating the expressions of the key enzymes in rutin biosynthesis， such as FLS， PAL and F3'5'H. This study provides reference for the future analysis of C2H2 zinc finger protein gene function of golden buckwheat.