2025-5-3- 20
Home > Archive>Volume 24, Issue 4, 2023 >1186-1193. DOI:10.13430/j.cnki.jpgr.20221124001
PDF HTML XML Export Cite reminder
Preliminary Identification and Molecular Marker Development of the Restorer-of-fertility Gene GmRf1 of CMS-RN Type Sterile Lines in Soybean
Author:
Affiliation:

1.College of Agronomy， Jilin Agricultural University， Changchun 130118;2.Soybean Research Institute， Jilin Academy of Agricultural Sciences/Key Laboratory of Hybrid Soybean Breeding of the Ministry of Agriculture and Rural Affairs， Changchun 130033

Fund Project:

Foundation projects： Science and Technology Development Plan Project of Jilin Province （20210302005NC）； Earmarked Fund for China Agriculture Research System（CARS-04）； Agricultural Science and Technology Innovation Project of Jilin Province （CXGC2021ZD002，CXGC2021RCY009）

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    The breeding of hybrid soybean varieties is mainly based on the three-line method （based on cytoplasmic male sterility）. As the male parent of the hybrid， the restorer-of-fertility （Rf ） gene in the restorer line plays a decisive role. In the former study， we mapped the Rf gene GmRf1 for the soybean RN type cytoplasmic male sterile line. This study conducted functional annotation， subcellular location prediction， sequence alignment and differential expression analysis of candidate genes in the physical interval of GmRf1. We identified that the PPR （Pentatricopeptide repeats） gene Glyma.16G161900 encoding for 576 amino acids encoded in male restorer line JLR230 is GmRf1. By crossing Williams82 containing GmRf1 with the female sterile line JLCMS204A， the pollen fertility of F1 plants was identified demonstrating that GmRf1 could restore the fertility of CMS-RN sterile lines. We further developed functional molecular markers Rf1-dCAPS-2 and Rf1-dCAPS-3 based on the single nucleotide mutation sites of GmRf1 between female and male parents. Collectively， this study laid a theoretical and technical foundation for the future marker-assisted selection of GmRf1， as well as the creation of new restorer lines through genetic engineering.

    Reference
    Related
    Cited by
Get Citation

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:November 24,2022
  • Revised:January 05,2023
  • Online: June 13,2023
  • Published: June 14,2023
Article QR Code
You are the th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.