The yield and seed mineral nutrient quality are essential factors in breeding of rice varieties. Identification of rice germplasm resources with high yield and high quality can provide elite parents or intermediate lines in breeding. This study measured eight yield-related traits （plant height， spike length， grain number per panicle， filled grain number per panicle， thousand kernels weight， grain weight per panicle， seed setting rate and grain density） and six mineral nutrient quality-related traits （zinc， iron， calcium， magnesium， copper and manganese content） in 139 japonica rice germplasm accessions， followed by the correlation analysis and principal component analysis （PCA）. The plant height， spike length， grain number per panicle， filled grain number per panicle and grain weight per panicle were positively correlated （highly significant）， and the content of mineral nutrient was positively correlated with each other in different degrees. The content of Zn and Mg was significantly and negatively correlated with grain weight per panicle. PCA using either eight or six indicators suggested three principal components （PCs）， respectively， with the cumulative contribution rate of 78.432% and 71.697%， respectively. Four yield key indicators and five mineral nutrient quality key indicators were selected based on the loadings of these indicators in CI1-3. Two germplasm accessions with high yield and high content of mineral nutrient， namely， Hungarian No.1 and Mu 10-815， were identified by combining the results of comprehensive evaluation of yield and mineral nutritional quality. The cluster analysis suggested four groups within this collection， including Group-I that contained only two germplasms with very high mineral nutrient content， Group-II that contained 41 germplasms with relatively high Zn content but poor yield traits. Group-III and IV with 69 and 27 germplasms respectively， exhibited superior yield traits. Group-III， however， had relatively low mineral nutrient content， whereas Group-IV had relatively high amounts of Mn and Ca content. Collectively， this study can provide theoretical basis and excellent germplasm for the selection and breeding of new high yield and high quality rice varieties.