The wheat grain hardness is an important index affecting the commodity classification， milling technology and flour final processing use. Molecular marker-assisted （MAS） technique can effectively improve the breeding efficiency for grain hardness in wheat. In order to explore and develop more molecular markers that are closely linked to the grain hardness of wheat， the recombinant inbred lines （RILs） population was constructed by crossing the hard wheat Yangmai 158 with soft wheat Xifeng， and genotyped by wheat 55K SNP array technique. The genetic linkage map is 2784.9 cM in length with 3830 non-consegregation SNP markers. Based on the kernel hardness phenotype in the 4-year experiment， the QTL affecting wheat grain hardness were mapped in the chromosome. A total of 12 repeatable QTL were identified on chromosomes 1A， 1B， 1D， 2A （2）， 3A， 4D， 5A， 5D， 6B， 6D and 7A， and each single QTL could explain 3.2%-15.2% of grain hardness variation. Among them， eleven QTL were derived from the soft wheat Xifeng， and one QTL from the hard wheat Yangmai 158. Seven QTL showed stable performance and were detected simultaneously in the 4-year experiment， and five QTL were newly identified. The newly discovered QTL， especially QTL on chromosome 5D， could account for up to 15.2% of the phenotypic variation. SNP markers closely linked to these QTL will provide help for marker-assisted selection in soft wheat in the middle and lower reaches of the Yangtze River.