Soybean is an important legume crop in the world， and serves as the main donor of protein and oil for human diet and animal feeding. The protein and oil content are genetically controlled by multiple genes， and are largely environment-dependent. It is of great significance to explore high protein and oil loci in targeted breeding of new soybean varieties. In this study， a genetic segregation population including 880 families was generated by Heinong 84 （high yield and quality） crossed with Jinghe 4 （high protein content）， and subjected for the protein and oil content quantification. The genotyping was conducted using ZDX1 SNP array and SSR molecular markers， combine. Two protein QTL and two oil QTL were identified through the complete interval mapping method （ICIM-ADD） using QTL IciMapping 4.2. qPro_11_1 and qOil_11_1 were co-localized in an interval of 126.27 kb between the molecular markers SSR_11_1087 and SSR_11_1090， showing the genetic contribution rate of 4.05% and 3.23% respectively， as well as five annotation genes in the region. qPro_14_1 and qOil_14_1 were co-localized between the molecular markers SSR_14_0421 and SSR_14_0429， with the interval size of 246.09 kb with 15 annotation genes， showing the genetic contribution rate of 4.67% and 7.13% respectively. Collectively， this study identified the QTL loci on protein content and oil content， thus laying a foundation for the marker-assisted selection breeding and gene map-based cloning of soybean with high protein and high oil content.