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Home > Archive>Volume 24, Issue 5, 2023 >1485-1497. DOI:10.13430/j.cnki.jpgr.20230329001
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Analysis of DNA Damage and Specific Gene Regulatory Network Induced by Lithium Ion Beam Irradiation in Wheat （Triticum aestivum L.）
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Affiliation:

1.College of Agriculture， Qingdao Agricultural University， Qingdao 266109，Shandong;2.Institute of Crop Sciences，Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/National Center of Space Mutagenesis for Crop Improvement， Beijing 100081;3.China Institute of Atomic Energy， Beijing 102413

Fund Project:

National Key Research and Development Program of China（2022YFD1200705）

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    Abstract:

    As a new mutagen， lithium （7Li） ion beam plays an increasingly important role in crop mutation breeding. In this study， the characteristics of wheat DNA damage induced by 7Li ion beam irradiation treatment were explored by comet assay， and the transcriptional re-programming was preliminarily analyzed by transcriptome analysis. The wheat seedlings showed lower growth inhibition of wheat seedlings caused by 7Li ion beam irradiation， but seedlings leaf vein chlorosis to cracking， if compared with those treated by the conventional mutagenic gamma ray （γ）. Based on GO and KEGG functional analysis of differentially expressed genes （DEGs） induced by irradiation， DEGs induced by 7Li ion beam irradiation were mainly enriched in cell wall synthesis and metabolism and glycerolipid metabolic pathways， while DEGs induced by γ ray irradiation were mainly enriched in photosynthetic metabolic pathways. That suggested that cell wall synthesis and metabolism and glycerolipid metabolic pathways are likely modulated by 7Li ion beam irradiation， while photosynthetic metabolic pathways are likely modified by γ ray irradiation. Gained from the results of transcription factor analysis of two radiation-induced DEGs， several transcription factor families， such as MYB， WRKY， bHLH and NAC， might specifically respond to 7Li ion beam irradiation. The results of this study implied that 7Li ion beam irradiation specifically modify the transcriptional re-programming of Whirly family transcription factors to regulate DNA damage repair， while the conventional γ ray irradiation likely induced the E2F / DP family transcription factors to regulate DNA damage repair.

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History
  • Received:March 29,2023
  • Revised:April 12,2023
  • Online: August 30,2023
  • Published: August 30,2023
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