Abstract:The macadamia nut fruits have rich diversity on their single fruit weight, seed yield, and kernel yield. In order to indentify the Macadamia germplasms with the best performance applicable for breeding programs, 100 macadamia germplasms were investigated in this study, and a comprehensive evaluation analysis was conducted on 12 fruit quantitative traits through statistics methods such as correlation, cluster analysis, and principal component analysis, etc. The results showed that the coefficient of variation of the 12 quantitative traits ranged from 9.66% to 23.62%, with a significant degree of variation on the traits of single fruit weight (23.62%) and kernel weight (21.20%), respectively. There was a highly significant positive correlation between single fruit weight and kernel weight, which is the important reference for selecting large fruits. Cluster analysis separated the 100 germplasms into two groups, with group Ⅰ having poor appearance of the fruits quality, such as small fruit size, relatively low seed rate and kernel yield rate; while group Ⅱ have good appearance of the fruits quality, such as large fruit size, high seed rate and kernel yield rate, which meets the market demands. Principal component analysis divided the 12 quantitative traits into 4 principal components, with a cumulative contribution rate of 79.902%. The initial eigenvalues and the cumulative contribution of the first principal component were 4.280 and 35.667%, respectively, and the components were mainly associated with the size of the fruit traits; the cumulative contribution of the second principal component was 20.177% and they were mainly associated with the kernel traits; the cumulative contribution of the third and the fourth principal component was 13.026% 11.032%, respectively, and they were mainly associated with the seed and kernel yield of the fruit, respectively. The comprehensive evaluation score of the fruit traits ranged from -2.333 to 0.983, and the top 10 excellent germplasms were identified as the following sequence, Pattern, SS14, Zhuangyuan 1, Lincang 47, Guire No. 1, Nanya 2, A4, B2, Lincang 1, and Gui 3. Notably, the FS3 was identified with the largest single fruit weight, the Guire No. 1 was identified with the heaviest kernel, the Breeding 8 was identified with the highest seed yield, and the A4 was identified with the highest kernel yield, respectively. These comprehensive evaluation data and identified exellent germplasms provide important references for future conventional or hybrid breeding in Macadamia.