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Home > Archive>Volume 24, Issue 6, 2023 >1744-1754. DOI:10.13430/j.cnki.jpgr.20230419003
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Bioinformatics and Gene Diversity Analysis of the DMP Genes in Soybean
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Affiliation:

1.School of Agronomy，Anhui Agricultural University，Hefei 230036;2.Institute of Crop Sciences， Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Soybean Biology in Beijing，Ministry of Ariculture and Rural Affairs， Beijing 100081;3.College of Life Sciences， Northeast Forestry University， Harbin 150040， Heilongjiang;4.College of Agriculture， Northeast Agricultural University， Harbin 150030， Heilongjiang

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Foundation project： The Innovation Project of Chinese Academy of Agricultural Sciences

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    Abstract:

    Double haploid techniques have been widely used to accelerate plant breeding， and in recent years， maize （Zea mays L.） lines containing mutations in the membrane protein （DMP） of the functionally unknown domain DUF679 have been used as haploidy-induced lines. In this study， soybean DMP genes with more than 60% homology with maize DMP gene were searched and bioinformatic analysis was performed. The molecular mechanism and biodiversity of GmDMP gene in soybean were investigated by using 2214 resequencing databases. The analysis results show that： GmDMP1 （Glyma.18G097400） and GmDMP2 （Glyma.18G098300） have high evolutionary affinity with maize DMP gene. The full length of genes is 645 bp， the homology rate of amino acid sequence is over 95%， and the number of encoded amino acids is completely consistent with the isoelectric point. The phosphorylation distribution differs at only one site. Both GmDMP1 and GmDMP2 genes have the same domain DUF679， which is more likely to locate in the ER， and both are transmembrane non-secreting hydrophilic proteins. There were 3 and 1 non-synonymous mutations of GmDMP1 and GmDMP2 genes in 2214 germplasm resources， respectively， consisting of 3 and 2 haplotypes. GmDMP1H3 has been strongly selected during domestication， and the mutation site of the GmDMP1H1 and GmDMP1H2 haplotypes is located in the DUF679 domain. It is possible to obtain a haploid inducible line by mutating two DMP genes in soybean， thus shortening the breeding life of soybean.

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History
  • Received:April 19,2023
  • Revised:May 13,2023
  • Online: October 31,2023
  • Published: October 31,2023
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