BIAN Jing
Daqing Branch of Heilongjiang Academy of Sciences, Daqing 163319WANG Xiaonan
Daqing Branch of Heilongjiang Academy of Sciences, Daqing 163319CAO Kun
Daqing Branch of Heilongjiang Academy of Sciences, Daqing 163319ZHAO Yue
Daqing Branch of Heilongjiang Academy of Sciences, Daqing 163319WANG Yunyun
Daqing Branch of Heilongjiang Academy of Sciences, Daqing 163319ZHANG Xiaoyan
Daqing Branch of Heilongjiang Academy of Sciences, Daqing 163319SUN Yufeng
Daqing Branch of Heilongjiang Academy of Sciences, Daqing 163319Daqing Branch of Heilongjiang Academy of Sciences, Daqing 163319
Foundation projects: Foreign Cooperation Project of Heilongjiang Academy of Sciences (DWHZ2023DQ01); Youth Innovation Fund of Heilongjiang Academy of Sciences (CXMS2023DQ01); Research Expenses of Provincial Research Institutes of Heilongjiang Province (CZKYF2021-2-A004, CZKYF2022-1-A004)
Cannabis is an annual herb and a versatile, sustainable crop, while uncovering the population diversity remain poorly investigated. In this study, the EST-SSR molecular markers were used to analyze the genetic diversity and population structure of cannabis. The results showed that a total of 113 score bands were amplified using 20 pairs of primers, of which all (100%) were polymorphic. A total of 232 alleles were detected, with an average of 4.0176 alleles per primer pair. The average observed heterozygosity (Ho) was 0.7102, and the average expected heterozygosity (He) was 0.6935. The Shannon information index of 200 individuals ranged from 0.7204 - 2.4625, with an average value of 1.5368. Polymorphism information content (PIC) ranged from 0.3519 to 0.8801, with an average of 0.6558. The mean gene flow (Nm) was 13.6525.Based on population genetic structure, principal component analysis and unweighted group analysis (UPGMA) with arithmetic mean, cannabis materials were grouped into 3 groups.. The results were similar between the clustering methods, but the distribution of minority individual plants was different among the three models. The results of clustering, genetic diversity and genetic similarity coefficient showed that cannabis individuals were closely related to each other. In addition, five pairs of core primers, which were able to distinguish the test germplasm, were deployed for the fingerprint construction. Collectively, these results provided a reference for the future breeding, genetic improvement and collection of core germplasm resources of cannabis.