Fusarium head blight （FHB） is a devastating disease of wheat， which seriously reduces grain yield and quality. It is an effective way to prevent the damage of FHB by introducing resistant genes into wheat varieties. In this study， the wheat line L06486 that carries the FHB resistance genes Fhb1， Fhb2， Fhb4 and Fhb5， was used as the donor and crossed with the variety Jimai 24， which was high-yield and widely adaptive across the Huanghuai area of wheat planting， but highly susceptible to FHB. Then the acquired plants were crossed with the dwarf wheat line 206A. A large spray facility was used to carry out the fog-way inoculation of spore-fluid through scab on the ears of wheat， across F₃ to F₆ multiple generations and populations. The resistance of the selected F₇ stable lines was validated by floret-inoculation and their resistant genes composition was identified with specific markers for Fhb1， Fhb2， Fhb4 and Fhb5. It showed that 106 F₇ lines had improved resistance to FHB in comparing with the recipient parent Jimai 24. Out of these 106 lines， 98 lines showed a range of moderate susceptible to high resistance， and 105 lines carried 1 to 4 FHB resistance genes each. The frequencies of Fhb1， Fhb2， Fhb4 and Fhb5 presence were 96.23%， 41.51%， 18.87% and 87.74%， respectively. Lines with single or multiple Fhb genes showed higher resistance to FHB than those with none of Fhb gene； the more Fhb genes it carried， the higher resistance to FHB. Altogether， these selected lines will be valuable in improving the FHB resistance in Huanghuai wheat planting region in the future.