2025-4-4- 13
Home > Archive>Volume 25, Issue 4, 2024 >647-659. DOI:10.13430/j.cnki.jpgr.20230807001
PDF HTML XML Export Cite reminder
Sequencing Analysis of Transcriptome in Different Parts of Nymphaea ‘Paul Stetson’ Flower
Author:
Affiliation:

Guangxi Subtropical Crops Research Institute/Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable， Ministry of Agriculture and Rural Affairs， Nanning 530001

Fund Project:

Foundation projects： Natural Science Foundation for Distinguished Young Scholars of Guangxi（2023GXNSFBA026222）； Basic Scientific Research Business Special Project of Guangxi Agricultural Science（Gui Nong Ke 2023YM11， Gui Nong Ke 2021YT152）

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    To investigate the floral aroma metabolism pathways and differentially expressed genes （DEGs） involved in the biosynthesis of terpenoid aroma compounds in tropical waterlily organs， the transcriptome sequencing was used to analyze the flower organs including the petal （PE）， stamen （ST） and pistil （PI） of N. ‘Paul Stetson’. The number of differentially expressed genes in PE-vs-PI， ST-vs-PI， and ST-vs-PE were 7853， 7501， and 2526， respectively. GO classification and enrichment analysis showed that these DEGs were mainly involved in biological regulation， cellular processes， metabolic processes， and stimulus response biological processes. KEGG annotation revealed abundant pathways with significantly enriched DEGs in PE-vs-PI， followed by ST-vs-PI， and ST-vs-PE. Ninety-eight of 794 DEGs that shared in three comparative groups were enriched in four terpenoid floral aroma synthesis pathways， and DEGs in PE-vs-PI and ST-vs-PI was higher than that in ST-vs-PE. In petals and stamens， the genes responsible for synthesis of acacia aldehyde and diterpenoid kaurene， were expressed at higher levels than in pistils. Six of 98 DEGs were randomly selected and subjected for qRT-PCR analysis， confirming the trend on transcriptional expression as revealed by transcriptome sequencing. The results provided a scientific reference for future deciphering the molecular mechanism of terpenoid aroma compounds biosynthesis in tropical waterlilies.

    Reference
    Related
    Cited by
Get Citation

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:August 07,2023
  • Revised:August 23,2023
  • Online: April 29,2024
  • Published: April 16,2024
Article QR Code
You are the th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.