Detection and Evaluation of Blast Resistance Genes in Backbone Indica Rice Parents from South China
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Biotechnology Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350003

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Foundation project: Fujian Province Public Welfare Scientific Research Program (2021R1027009,2022R1027003)

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    Abstract:

    By deployment of 11 functional markers of blast resistance genes, including Pi2 Piz-t Pi9, Pi25Pi5Pita PiaPtrPi1Pikm, and Pi54, this study analyzed the resistance gene diversity in the newly-developed 90 indica rice varieties from south China. The results showed that 0, 3, 35, 38, 14, and 0 varieties exhibited high-resistance, resistance, moderate resistance, moderate susceptibility, susceptibility, and high-susceptibility, respectively. The results tested for blast resistance were highly correlated in years. By analyzing the correlation among different detection stages, significant positive correlations in seedling blast and leaf blast, seedling blast and neck blast, as well as leaf blast and neck blast were observed. Except Pi9, the frequency of other 10 resistance genes in population were 75.56% (Pi54), 70.0% (Pi5), 47.78% (Pi2), 31.11% (Pi25 and Pia), 20.0% (Ptr), 15.56% (Pi1), 13.32% (Pita), 4.44% (Pikm) and 1.11% (Piz-t), respectively. Moreover, the distribution frequency of 11 resistance genes varied significantly among parents from different provinces. Further results also indicated that as the number of resistance genes increased, the level of resistance of parents showed a corresponding upward trend. Furthermore, the contribution of different resistance gene combinations to improve the disease resistance of rice parents showed significant differences. The current study provided a basis for the rational distribution of resistant conventional indica rice varieties with different genotypes in South China.

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History
  • Received:August 28,2023
  • Revised:October 23,2023
  • Adopted:
  • Online: March 05,2024
  • Published: February 26,2024
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