WRKY transcription factor is one of the most abundant transcription factors in higher plants， and play an important role in plant growth and development， senescence， abiotic and biological stress. O. granulata （Oryza meyeriana subsp. Granulata）， which serves as a closely related wild species of cultivated rice， represents the characteristics of shade tolerance， drought tolerance and high resistance to bacterial blight， becoming an important germplasm resources for cultivated rice improvement. In this study， using HMMER， Pfam， SMART， TBtools， NCBI software and website， 94 genes encoding WRKY transcription factors （OgWRKYs） were identified in O. granulata， which were unevenly distributed on 12 chromosomes. According to the number of WRKY domains and the characteristics of zinc finger structure， they were divided into groups Ⅰ， Ⅱ， Ⅲ and Ⅳ. Group II had the most members （52）， which was similar to other species. In addition to the conserved WRKYGQK heptaeptides， six variants were identified， among which WRKYGHK， WRRYGQK， WRKYAKK and WRKYSQK were new variants reported for the first time in plants. According to conserved domain analysis， OgWRKY61， OgWRKY71 and OgWRKY77a may be associated with plant disease resistance.KEGG pathway enrichment analysis showed that 14 OgWRKY transcription factors were enriched in plant-pathogen interaction pathways， and 10 of them were simultaneously enriched in MAPK signaling pathways. Further analysis of cis-acting elements suggested that OgWRKY30b， OgWRKY53， OgWRKY88， OgWRKY96 and OgWRKY111 might play important roles in response to biological and abiotic stresses in O. granulata. The results of qRT-PCR analysis showed that the expression of OgWRKY30b， OgWRKY53， OgWRKY88 and OgWRKY111 genes were induced by PXO99， while the expression of OgWRKY96 was inhibited by infection. The results of this study provided important reference for future mining of OgWRKYs genes in O. granulata.