Abstract:Based on "The Third National Campaign of Crop Germplasm Census and Collection", genetic diversity analysis, correlation analysis, cluster analysis and principal component analysis were used to study and evaluate 136 sorghum germplasm resources collected from 2020 to 2021 in Hebei province. The results showed that there were abundant phenotypic variations in the collection. The diversity index of 15 phenotypic traits ranged from 0.0844 to 1.9926, and the coefficient of variation ranged from 4.69% to 68.00%. The genetic diversity of 1000-grain weight and the coefficient of variation of panicle shape were the highest. A significant positive correlation between plant height and panicle traits was detected. These germplasms were divided into three groups by cluster analysis, while three clusters didn’t correlate with the geographic collection sites. Cluster I showed the best performance in panicle traits and could be used in breeding for technical purposes. The plant height of cluster II was low, which can be used as dwarf resources in germplasm innovation. Cluster III showed the best yield traits which can be used in breeding for higher grain production. Principal component analysis simplified phenotypic trait factors into 5 principal components, with the cumulative contribution rate of 60.182%. The plant height, the panicle and grain traits were the main factors contributing to the phenotypic differences. Based on the comprehensive scores of 136 sorghum germplasms that ranged from 0.107 to 1.147, the top 10 elite germplasm resources such as Suning sorghum, Changsui sorghum, Tiaozhou sorghum and Luoshu were selected. Collectively, based on the evaluation of these newly collected sorghum germplasm resources in Hebei province, this study provided insights for the mining of elite sorghum germplasm and the innovative utilization of germplasm.