Application of CRISPR/Cas9 Technology in Tropical Crops Breeding

doi:10.13430/j.cnki.jpgr.20231010001

2025-5-21- 11

Home > Archive>Volume 25, Issue 3, 2024 >312-322. DOI:10.13430/j.cnki.jpgr.20231010001

Application of CRISPR/Cas9 Technology in Tropical Crops Breeding
DOI:
                        10.13430/j.cnki.jpgr.20231010001
                    
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Author:
                        WANG Jingyi1WANG Jingyi
Key Laboratory of Tropical Crops Biology and Genetic Resources，Ministry of Agriculture and Rural Affairs， Institute of Tropical Bioscience and Biotechnology， Chinese Academy of Tropical Agricultural Sciences/ National Key Laboratory for Tropical Crop Breeding， Sanya Research Institute / Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources， Hainan Institute for Tropical Agricultural Resources， Haikou 571101
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GAN Shanshan1,2GAN Shanshan
Key Laboratory of Tropical Crops Biology and Genetic Resources，Ministry of Agriculture and Rural Affairs， Institute of Tropical Bioscience and Biotechnology， Chinese Academy of Tropical Agricultural Sciences/ National Key Laboratory for Tropical Crop Breeding， Sanya Research Institute / Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources， Hainan Institute for Tropical Agricultural Resources， Haikou 571101;College of Horticulture and Forestry Sciences， Huazhong Agricultural University， Wuhan 430000
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JIA Caihong1JIA Caihong
Key Laboratory of Tropical Crops Biology and Genetic Resources，Ministry of Agriculture and Rural Affairs， Institute of Tropical Bioscience and Biotechnology， Chinese Academy of Tropical Agricultural Sciences/ National Key Laboratory for Tropical Crop Breeding， Sanya Research Institute / Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources， Hainan Institute for Tropical Agricultural Resources， Haikou 571101
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LIU Juhua1LIU Juhua
Key Laboratory of Tropical Crops Biology and Genetic Resources，Ministry of Agriculture and Rural Affairs， Institute of Tropical Bioscience and Biotechnology， Chinese Academy of Tropical Agricultural Sciences/ National Key Laboratory for Tropical Crop Breeding， Sanya Research Institute / Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources， Hainan Institute for Tropical Agricultural Resources， Haikou 571101
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Affiliation:1.Key Laboratory of Tropical Crops Biology and Genetic Resources，Ministry of Agriculture and Rural Affairs， Institute of Tropical Bioscience and Biotechnology， Chinese Academy of Tropical Agricultural Sciences/ National Key Laboratory for Tropical Crop Breeding， Sanya Research Institute / Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources， Hainan Institute for Tropical Agricultural Resources， Haikou 571101;2.College of Horticulture and Forestry Sciences， Huazhong Agricultural University， Wuhan 430000
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Fund Project:Foundation projects： Hainan Provincial Natural Science Foundation （321RC638）； Chinese Academy of Tropical Agricultural Sciences for Science and Technology Innovation Team of National Tropical Agricultural Science Center （CATASCXTD202310）； National Key Laboratory for Tropical Crop Breeding （NKLTCB202301）； The Innovation Platform for Academicians of Hainan Province （YSPTZX202101）； Earmarked Fund for Modern Agro-industry Technology Research System （CARS-31）

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Abstract:

Tropical crops， including banana， papaya， sugarcane， cassava， rubber tree， oil palm， etc， are of importance in Chinese agriculture， which not only provide raw materials for our daily life and industrial and agricultural production， but also contribute to the main agricultural output and economic growth in tropical and subtropical zones of China. There are many barriers in tropical crops in use of modern molecular breeding techniques， such as polyploidy， heterozygous， vegetative propagation， long juvenile phase and large size of plants， etc. The genetic improvement of tropical crops through conventional breeding is troublesome， time-consuming， low efficiency and less progress. The development of genome editing technologies has brought a new way in tropical crops breeding. CRISPR-Cas9 mediated genome editing has been widely used in plants， profited from its higher targeting efficiency， versatility and ease of usage. This approach has been applied in banana， cassava， rubber tree， and sugarcane. Here， we focus on the recent advances based on CRISPR/Cas9 methodologies， and summarize their application in tropical crops breeding， as well as propose future perspectives and challenges in improving tropical plants.

Key words:tropical crops;genome editing;CRISPR/Cas9

Reference

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Received:October 10,2023
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