This study aims to identify the pre-harvest sprouting resistance in wheat germplasm resources and molecular markers for marker-assisted selection， ultimately obtaining excellent white grain wheat germplasm resources applicable in wheat breeding. These spikes of 222 wheat germplasm resources were tested for pre-harvest sprouting resistance， and the functional markers of eight anti-pre-harvest sprouting genes， including myb10D， DFR-B， Vp1B3， PM19-A1， MFT-3A， MFT-A2， MKK3-A， QSD1， were used for genotyping. Phenotypic identification results showed that there were significant differences in the relative sprouting percentage of 222 wheat germplasm resources. The relative sprouting percentage ranged from 0 to 1.15， and the average relative sprouting percentage was 0.73. 38 wheat materials were identified to be resistant to pre-harvest sprouting， including 9 white wheat， 27 red wheat and 2 black wheat. Correlation analysis between allele types and relative sprouting percentage showed that the relative sprouting percentage was significantly correlated with functional markers myb10D， DFR-B， Vp1B3， MFT-3A and MFT-A2， but not with PM19-A1， MKK3-A and QSD1. These results indicated that molecular markers myb10D， DFR-B， Vp1B3， MFT-3A and MFT-A2 could be used for detection of pre-harvest sprouting resistance and marker-assisted breeding. Based on the phenotype and genotype results， nine white wheat germplasm resources with pre-harvest sprouting resistance were selected， including Yunong 914， Yunong 946， Fengdecunmai 30， Fangmai 5， Xumai 029， Lianmai 1901， Baofeng 1903， Zhengmai 829 and 13wang27-8， which could be used for genetic breeding and layout of wheat varieties with pre-harvest sprouting resistance.