Genetic Diversity Analysis of Aroma Substances in 62 Grape Germplasm Resources
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1.College of Horticulture, Agricultural University of Shanxi, Jinzhong 030800;2.Institute of Fruit Trees, Shanxi Agricultural University/Shanxi Key Laboratory of Fruit Germplasm Creation and Utilization, Taiyuan 030031

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Foundation projects: Precision Identification Project of Crop Germplasm Resources,Ministry of Agriculture and Rural Affairs (19221993); Ministry of Agriculture and Rural Affairs Germplasm Resource Collection and Preservation Project (19221876); Shanxi Provincial Key Talent Team for Science and Technology Innovation (202204051002037);Biological Breeding Engineering Project of Shanxi Agricultural University (YZGC032)

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    Abstract:

    To investigate the genetic diversity of aroma components in grape germplasm resources, 62 grape germplasm resources were used as experimental materials. Headspace solid-phase microextraction combined with gas chromatography-mass spectrometry was used to detect volatile compounds, and significance analysis of aroma compounds was conducted. 8 agronomic traits were identified, such as color of the upper side, sheen on upper side, anthocyanin colouration of young leaves, articular flavor of mature berries, and so on. Correlation analysis was carried between volatile compounds and 8 agronomic traits. The characteristic volatile substances compounds was screened out by non-metric multidimensional scale analysis (NMDS, stress<0.2) and orthogonal partial least squares discriminant analysis (OPLS-DA). The results showed that a total of 99 volatile substances in 7 categories were detected in 62 grape germplasm resources, mainly alcohols, esters and terpenes, with the contents of 13.83 mg/L, 13.53 mg/L and 8.99 mg/L, respectively. There are significant differences among various volatile substances. The results of correlation analysis between 8 agronomic traits and aroma substances showed that the color of the upper side,sheen on upper side, anthocyanin colouration of young leaves, color of skin and articular flavor of mature berries were significantly correlated with terpenes, esters and ketones. Non-metric multidimensional scaling analysis can clearly distinguish the germplasms with very weak anthocyanin coloring of young leaves and the germplasms with middle anthocyanin coloring of young leaves. Based on the variable importance in projection (VIP)>1, P<0.05 as the standard, 17 main volatile substances were screened, among which the VIP value of ethyl caproate was the highest (4.07), and the VIP value of nerol in terpenes was the highest (2.07). This study provides a theoretical reference for improving the aroma quality of berries and the innovation of grape germplasm resources.

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History
  • Received:November 22,2023
  • Revised:
  • Adopted:
  • Online: July 04,2024
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