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Home > Archive>Volume 25, Issue 7, 2024 >1153-1162. DOI:10.13430/j.cnki.jpgr.20231203001
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Identification of Sex Determination-Related Genes from the Monoecious Kiwifruit Flowers
Author:
Affiliation:

1.College of Horticulture， Fujian Agriculture and Forestry University， Fuzhou 350002;2.Chinese Academy of Sciences Wuhan Botanical Garden/Chinese Academy of Sciences Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture/Chinese Academy of Sciences Engineering Laboratory for Kiwifruit Industrial Technology， Wuhan 430074;3.College of Advanced Agricultural Sciences， University of Chinese Academy of Sciences， Beijing 100049

Fund Project:

Foundation projects： Strategic Priority Research Program of the Chinese Academy of Sciences （Category A） （XDA24030404）； The Earmarked Fund for CARS（CARS-26）； Supported by Hubei Hongshan Laboratory（2021hszd017）；The Key R&D Program of Shaanxi Province （2023-ZDLNY-24）； Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees （GSSZ202301）

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    Abstract:

    In order to explore the molecular mechanism of the variation of kiwifruit from dioecious to monoecious， transcriptome sequencing， bioinformatics analysis and qRT-PCR verification on female and male flowers of the monoecious mutant of Mantianhong kiwifruit were carried out. The results of transcriptome differential expression gene identification showed that there were 337 differentially expressed genes （DEGs） between female and male flowers， with 241 genes up-regulated and 96 genes down-regulated in male flowers. The GO and KEGG enrichment of DEGs showed that compared with female flowers， the up-regulated genes in male flowers were mainly involved in the metabolism of amino sugars and nucleotide sugars and the synthesis of secondary metabolites. The down-regulated genes were mainly enriched in secondary metabolite synthesis， carotenoid synthesis and other pathways. Through functional annotation analysis， 30 potential gender-related regulatory genes were identified， five of which were expressed in the secondary metabolite synthesis pathway. Seven DEGs were selected for qRT-PCR， and their expression levels in male and female flowers were in accordant with those in transcriptome data. The results of this study provide a theoretical basis for the creation of kiwifruit monoecious materials.

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  • Received:December 03,2023
  • Online: July 04,2024
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