Polianthes tuberose L. is a perennial bulbous flower of the Amaryllidaceae family. It is a famous fragrant flower plant with high ornamental， economic and medicinal values. Bulbs are their main reproductive and medicinal organs. In order to further explore its growth and development mechanism and secondary metabolic pathways， we use the high-throughput sequencing PacBio Sequel platform to make use of its bulbs as materials， the single molecule long reading sequencing technology （SMART） was used to sequence and analyze the full-length transcriptome of Agave amica bulbs for three generations. The platform obtained a total of 508012 high-quality CCS reads and 402032 full-length non chimeric sequences （FLNC）， and clustered the full-length non chimeric sequences to obtain a total of 137215 high-quality consistent sequences. After quality control and redundancy removal， a total of 81719 transcripts （Unigene） and 36215 complete CDS regions were obtained. All transcripts obtained were annotated and functionally classified through databases such as NR， SwissProt， KOG，KEGG GO， etc. The result showed that a total of 64362 single genes were annotated， with NR having the highest annotation count of 63538， accounting for 98.72%，while KEGG had the lowest number of annotated genes，with 58878 genes annotated to 148 pathways. And 41240 SSRs， 15835 lncRNAs and 4903 transcription factors were analyzed. RLK transcription factor family has the most genes. The discovery of homologous box transcription factors family may be involved in the development process of tuber， PtKNOX1L13 gene was cloned，its expression levels were relatively high during the initiation， enlargement， and maturity stages of tubers， transient overexpression indicates that it can promote the development of bulbils，so that it may be an important functional gene involved in bulb formation and development.This study provides reference and basis for in-depth research and development of molecular markers， important functional genes and germplasm resources innovation of Polianthes tuberose L.