2025-4-6- 19
Home > Archive>Volume 25, Issue 10, 2024 >1767-1780. DOI:10.13430/j.cnki.jpgr.20231215003
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Transcriptomics-Metabolomics Combined Analysis Highlight the Anthocyanin Biosynthesis Mechanism of Red Testa in Peanut（Arachis hypogaea L.）
Author:
Affiliation:

1.Agronomy College， Hebei Agricultural University/North China Key Laboratory for Crop Germplasm Resources of Education Ministry/Hebei Germplasm Resources Laboratory， Baoding， 071000;2.Puyang Academy of Agricultural and Forestry Sciences， Puyang 457000，Henan;3.Chengde Academy of Agriculture and Forestry Sciences， Chengde 067000， Hebei

Fund Project:

Foundation projects： Hebei Province Modern Agricultural Industrial Technology System Construction Special Fund（HBCT2024040201）； Science and Technology Research Project of Colleges and Universities in Hebei Province（ZD2022069）； Project of Youth Top Talent Funding in Hebei Province（0602015）； Key Project of Science and Technology Research of Modern Seed Industry of the Department of Science and Technology in Hebei Province（19226363D）； Project of the Construction of Zhuangzi River Taihang Mountain Agricultural Innovation Station in Quyang（903-311718001）

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    Abstract:

    Peanut is the important unique export agricultural product， which occupies a vital position in agricultural development of China.In this study， two peanut varieties， Hongzhenzhu （H） and Baizhenzhu （B， the control）， were used as research samples for transcriptomic-metabolomics combined analysis. At 30 and 45 days after flowering， the testa color （L value， a value， b value） and anthocyanin content of Hongzhenzhu and Baizhenzhu were extremely significantly different between varieties. FPKM hierarchical cluster analysis showed that compared with Baizhenzhu， there were 1847 and 1843 unique genes at 30 and 45 days after flowering in Hongzhenzhu， respectively. GO analysis annotation results showed there were 8 GO terms significantly related to anthocyanin synthesis. Among them， GO：0055114 and GO：0016207 had enriched with 8 （C4H、two CHS、F3′H、two FLS、F3H and PAL）and 7（two CHS、CHI、F3′H、two FLS and F3H） differential expressed geness respectively. The results of KEGG enrichment analysis showed that 6 metabolic pathways were significantly related to anthocyanin biosynthesis， respectively. Metabolomics results showed that cyanidin， procyanidin， petunidin， delphinidin， malvidin， peony （peonidin） and their derivatives were differential accumulated metabolites （DAMs）. The transcriptomics-metabolomics combined analysis showed that flavonoid biosynthesis （ko00941） is the key synthetic pathway and delphin and centaurea are the key DAMs of testa color formation. The qRT-PCR result of 11 detected DEGs was consistent with the results of transcriptome sequencing. These results of this study have a certain reference significance for revealing the regulatory mechanism of anthocyanin synthesis in peanut testa.

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  • Received:December 15,2023
  • Online: October 09,2024
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