Mung bean sprout is rich in vitamins， dietary fiber and other nutrients， and developing elite cultivars for sprout could improve the quality and commercial value on sprout production. Thirty-five new lines were gained from the previously-developed new lines evaluated for the sprout characters using the core collection of mung bean. In this study we evaluated the characters for sprout production of 35 new elite lines. The results revealed abundant variations on sprout length （5.56-7.42 cm， coefficient of variation （CV）=13.36%）， and sprout thick （2.41-3.24 mm，CV=13.00%）， while a fairly low variation on production ratio （8.12-10.36， CV=3.60%）. Significant positive correlation was found between production ratio and sprout thick （R=0.817）， while negative correlation was found between production ratio and 100-seed weight （R=-0.583） or sprout length （R=-0.137）. Multiple regression showed that sprout thick had greater a contribution to sprout weight than sprout length and seed size too. Finally， lines 21XJ54-1 and 21BJ yu95， 21XJ54-2 that suitable for slim sprout and lines 21BJ yu96， 21DT46 and Zhonglv27 that suitable for thick sprout were selected. In addition， seeds of Zhonglv27 harvested from diverse eco-regions were observed to have different sprout production ratio. The seeds from Fengning city of Hebei province had the highest production ratio （10.27）， followed by Xinxiang city of Henan province （9.94）. Seeds from Changji city of Xinjiang autonomous region had the highest value on sprout length （6.23 cm）， while Fengning of Hebei province was the highest on sprout thick （3.70 mm）. Collectively， this study provided elite germplasm for breeding of new mung bean varieties for sprout production.