SHANG Kun
Guizhou Institute of Subtropical Crops, Guizhou Academy of Agricultural Sciences, Guiyang 550025FU Yuhua
Guizhou Institute of Subtropical Crops, Guizhou Academy of Agricultural Sciences, Guiyang 550025LI Xiushi
Guizhou Institute of Subtropical Crops, Guizhou Academy of Agricultural Sciences, Guiyang 550025MENG Qiuyi
Guizhou Institute of Subtropical Crops, Guizhou Academy of Agricultural Sciences, Guiyang 550025YANG Lingling
Guizhou Institute of Subtropical Crops, Guizhou Academy of Agricultural Sciences, Guiyang 550025ZHU Jiabao
Cotton Institute, Anhui Academy of Agricultural Sciences, Hefei 2300311.Guizhou Institute of Subtropical Crops, Guizhou Academy of Agricultural Sciences, Guiyang 550025;2.Cotton Institute, Anhui Academy of Agricultural Sciences, Hefei 230031
Foundation projects: National Natural Science Foundation of China(32360480);Guizhou Academy of Agricultural Sciences Post National Foundation Subsidy Projects ([2021] 24);Germplasm Resources Project of Guizhou Academy of Agricultural Sciences ([2023] 008);Guizhou Academy of Agricultural Sciences Youth Science and Technology Fund Project ([2020] 06);Open Project Fund of Key Laboratory of Plant Resources Protection and Germplasm Innovation of the Ministry of Education (Guizhou University) (Qianjiaohe KY [2022] 368)
Coicis oil is one of the main functional substances of coix seed, and fatty acid is an important component. The contents of stearic acid, oleic acid and linoleic acid in seed kernels of 190 coix germplasms from 9 provinces in China were detected. ClSAD and ClFAD2 sequences polymorphisms were analyzed and the haplotypes were identified. The haplotype association analysis of fatty acid content was carried out. The results showed that there were wide variations in the content of three fatty acids in the seeds of different coix germplasms, the coefficient of variation was 15.84 % - 23.05 %, and the genetic diversity index was 5.22 - 5.23, among which the content of oleic acid was the highest and the content of stearic acid was the lowest, and there was a very significant positive correlation between the fatty acid components. There were 14 SNPs and 3 SNPs in ClSAD and ClFAD2, respectively, and 5 haplotype have been identified. The ClSAD haplotype Hap3 and ClFAD2 haplotype Hap1 are consistent with the reference genome, respectively. Hap3 of ClSAD was significantly associated with stearic acid content and had a negative effect on the conversion of stearic acid to oleic acid. Hap1 of ClFAD2 was favorable for linoleic acid accumulation, while Hap2 was significantly associated with linoleic acid content and had negative effects on linoleic acid synthesis. One key SNP locus was identified in each of the two genes, which was the key locus to the difference of SAD and FAD2 enzyme activity. The results will provide a theoretical basis for the breeding of high oil coix varieties, as well as the development of molecular markers and the analysis of related molecular mechanisms.