2025-4-4- 19
Home > Archive>Volume 25, Issue 2, 2024 >184-192. DOI:10.13430/j.cnki.jpgr.20230718001
PDF HTML XML Export Cite reminder
The Resistance Investigation and Mapping Analysis of Fusarium Crown Rot for Wheat Accessions
Author:
Affiliation:

1.College of Agronomy， Henan Agricultural University/State Key Laboratory of Wheat and Maize Crop Science， Zhengzhou 450046;2.Nanyang Academy of Sciences， Nanyang 473000， Henan

Fund Project:

Foundation projects： Henan Major Science and Technology Project （201100110100）；National Key Research and Development Program （2019YFE0118300）； Key Scientific and Technology Projects of Henan Province（222102520002）； China Postdoctoral Science Foundation（2022M711066）

  • Article
    • | |
  • Metrics
    • |
  • Reference [32]
    • | | | |
  • Comments
    Abstract:

    By applying the millet grains as pathogen medium， we inoculated the 163 wheat germplasm accessions which widely cultivated currently. No immune or highly resistant wheat accessions were identified， while the resistance variations estimated by disease index （DI） were found following a normal distribution 13.28-83.33 in greenhouse and 10.27-73.89 in field， respectively. The correlation coefficient， based on the phenotype collected from greenhouse seedling assay and field trials was 0.79， supporting a high correlation on resistance at the seedling and adult stages. Furthermore， the genome-wide association study （GWAS） found significant SNPs distributed on all chromosomes of wheat， with enrichment on chromosome 2A with an interval from 725 Mb to 763 Mb. This was in keeping with the results of the bulked segregant analysis （BSA）， which revealed significant SNPs distributed from 730 Mb to 750 Mb on chromosome 2A. GWAS and BSA indicated a significant resistance locus， which resided from 730 Mb to 750 Mb on chromosome 2A and associated with fusarium crown rot （FCR） infection. This study can provide important reference for identifying resistant wheat materials and exploring FCR resistant loci in wheat.

    Reference
    [1] Yang X, Pan Y, Singh P K, He X Y, Ren Y, Zhao L, Zhang N, Cheng S H, Chen F. Investigation and genome-wide association study for Fusarium crown rot resistance in Chinese common wheat. BMC Plant Biology, 2019, 19(1):153
    [2] Pariyar S R, Erinbas-Orakci G, Dadshani S, Chijioke O B, Leon J, Dababat A A, Grundler F M W. Dissecting the genetic complexity of Fusarium crown rot resistance in wheat. Scientific Reports, 2020, 10(1):3200
    [3] Kazan K, Gardiner D M. Fusarium crown rot caused by Fusarium pseudograminearum in cereal crops: Recent progress and future prospects. Molecular Plant Pathology, 2018, 19(7): 1548
    [4] Backhouse D, Abubakar A A, Burgess L W, Dennis J I, Hollaway G J, Wildermuth G B, Wallwork H, Henry F J. Survey of Fusarium species associated with crown rot of wheat and barley in eastern Australia. Australasian Plant Pathology, 2004, 33(2): 255
    [5] Kettle A J, Batley J, Benfield A H, Manners J M, Kazan K, Gardiner D M. Degradation of the benzoxazolinone class of phytoalexins is important for virulence of Fusarium pseudograminearum towards wheat. Molecular Plant Pathology, 2015, 16(9): 947
    [6] Smiley R W. Fusarium crown rot whitehead symptom as influenced by wheat crop management and sampling date. Plant Disease, 2019, 103(10): 2612
    [7] Sohail Q, Erginbas O G, Ozdemir F, Jighl Y A, Dreisigacker S, Bektas H, Birisik N, Ozkan H, Dababat A A. Genome-wide association study of root-lesion nematodes pratylenchus species and crown rot Fusarium culmorum in bread wheat. Life, 2022, 12(3): 372
    [8] Xu F, Yang G Q, Wang J M, Song Y L, Liu L L, Zhao K, Li Y H, Han Z H. Spatial distribution of root and crown rot fungi associated with winter wheat in the North China Plain and its relationship with climate variables. Frontiers in Microbiology, 2018, 9:1054
    [9] Fan X F, Yan Z, Yang M X, Waalwijk C, Lee T, Diepeningen A V, Brankovics B, Chen W Q, Feng J, Zhang H. Contamination and translocation of deoxynivalenol and its derivatives associated with Fusarium crown rot of wheat in Northern China. Plant Disease, 2021, 105(11): 3397
    [10] Mandala G, Geoloni C, Busato I, Favaron F, Tundo S. Transgene pyramiding in wheat: Combination of deoxynivalenol detoxification with inhibition of cell wall degrading enzymes to contrast Fusarium Head Blight and Crown Rot. Plant Science, 2021, 313: 111059
    [11] Beccari G, Prodi A, Pisi A, Senatore M T, Balmas V, Tini F, Onofri A, Pedini L, Sulyok M, Brocca L, Covarelli L. Cultivation area affects the presence of fungal communities and secondary metabolites in Italian durum wheat grains．Toxins,2020,12 (2):97
    [12] Duan S N, Jin J J, Gao Y T, Jin C L, Mu J Y, Zhen W C, Sun Q X, Xie C J, Ma J. Integrated transcriptome and metabolite profiling highlights the role of benzoxazinoids in wheat resistance against fusarium crown rot. The Crop Journal, 2022, 10(2): 407
    [13] Jin J J, Duan S N, Qi Z Y, Zhen W C, Ma J. Identification of proteins associated with Fusarium crown rot resistance in wheat using label-free quantification analysis. Journal of Integrative Agriculture, 2021, 20(12): 3209
    [14] 崔晓敬,陈洁,岳建超. 小麦茎基腐病的发生与综合防治. 园艺与种苗, 2022, 7: 64-65Cui X J, Chen J, Yue J C. Occurrence and comprehensive control of wheat crown rot. Horticulture & Seed, 2022, 7: 64-65
    [15] 李怡文,李桂香,黄中乔,苗建强,刘西莉. 假禾谷镰孢引起的小麦茎基腐病发生危害与防控研究进展. 农药学学报, 2022, 24(5): 949-961Li Y W, Li G X, Huang Z Q, Miao J Q, Liu X L. Research progress on the occurrence, damage and prevention of Fusarium crown rot caused by Fusarium pseudograminearum. Chinese Journal of Pesticide Science, 2022, 24(5): 949-961
    [16] 赵利民,吕国强,何洋,冯贺奎,彭红. 河南省小麦茎基腐病发生现状及综合防控措施. 中国植保导刊, 2022, 42(05): 49-51Zhao L M, Lv G Q, He Y, Feng H K, Peng H. Occurrence status and integrated control measures of wheat crown rot in Henan province. China Plant Protection, 2022, 42(5): 49-51
    [17] 杨云,贺小伦,胡艳峰,侯莹,牛亚娟. 黄淮麦区主推小麦品种对假禾谷镰刀菌所致茎基腐病的抗性. 麦类作物学报, 2015, 35(3): 339-345Yang Y, He X L, Hu Y F, Hou Y, Niu Y J. Resistance of wheat cultivars in Huang-Huai region of China to crown rot caused by Fusarium pseudograminearum. Journal of Triticeae Crops, 2015, 35(3): 339-345
    [18] Ren Y, Hou W X, Lan C X, Basnet B, Singh R P, Zhu W, Cheng X Y, Cui D Q, Chen F. QTL analysis and nested association mapping for adult plant resistance to powdery mildew in two bread wheat populations. Frontiers in Plant Science, 2017, 8: 1212
    [19] Lan C X, Basnet B R, Singh R P, Huerta-Espino J, Herrera-Foessel S A, Ren Y, Randhawa M S. Genetic analysis and mapping of adult plant resistance loci to leaf rust in durum wheat cultivar Bairds. Theoretical & Applied Genetics International Journal of Breeding Research & Cell Genetics, 2017, 130(3):609
    [20] Wallwork H, Butt M, Cheong J P E, Williams K J. Resistance to crown rot in wheat identified through an improved method for screening adult plants. Australassian Plant Pathology, 2004, 33: 1-7
    [21] Poole G J, Smiley R W, Paulitz T C, Walker C A, Carter A H, See D R, Garland-Campbell K. Identification of quantitative trait loci (QTL) for resistance to Fusarium crown rot (Fusarium pseudograminearum) in multiple assay environments in the Pacific Northwestern US. Theoretical and Applied Genetics, 2012, 125(1): 91-107
    [22] Jin J J, Duan S N, Qi Y Z, Yan S H, Li W, Li B Y, Xie C J, Zhen W C, Ma J. Identification of a novel genomic region associated with resistance to Fusarium crown rot in wheat. Theoretical and Applied Genetics, 2020, 133 (7): 2063-2073
    [23] Yang X, Zhong S B, Zhang Q J, Ren Y, Sun C W, Chen F. A loss-of-function of the dirigent gene TaDIR-B1 improves resistance to Fusarium crown rot in wheat. Plant Biotechnology Journal, 2021, 19 (5): 866-868
    [24] Mitter V, Zhang M C, Liu C J, Ghosh R, Ghosh M. A high-throughput glasshouse bioassay to detect crown rot resistance in wheat germplasm. Plant Pathology, 2006, 55(3): 433
    [25] 陆宁海,吴利民,郎剑锋,杨蕊,李营营. 小麦茎基腐病苗期抗病性鉴定. 贵州农业科学, 2015, 43(10): 119-121Lu N H, Wu L M, Lang J F, Yang R, Li Y Y. Identification and of different wheat seedlings with resistance to crown rot. Guizhou Agricultural Sciences, 2015, 43(10): 119-121
    [26] Sun C W, Zhang F Y, Yan X F, Zhang X F, Dong Z D, Cui D Q, Chen F. Genome-wide association study for 13 agronomic traits reveals distribution of superior alleles in bread wheat from the Yellow and Huai Valley of China. Plant Biotechnology Journal, 2017, 15(8): 953-969
    [27] 耿君佑,陈建辉,董中东,任妍,张宁,孙丛苇,陈锋,赵磊.小麦芒性基因的定位与候选基因分析.植物遗传资源学报,2021, 22 (4): 1090-1098Geng J Y, Chen J H, Dong Z D, Ren Y, Zhang N, Sun C W, Chen F, Zhao L. Mapping and candidate gene analysis of awn type in common wheat. Journal of Plant Genetic Resources, 2021, 22 (4): 1090-1098
    [28] 周淼平,姚金保,张鹏,余桂红,马鸿翔. 小麦抗茎腐病种质筛选及鉴定新方法的建立. 植物遗传资源学报, 2016, 17(2): 377-382Zhou M P, Yao J B, Zhang P, Yu G H, Ma H X. Screening of germplasm and establishment of new evaluation method for the resistance to wheat crown rot. Journal of Plant Genetic Resources, 2016, 17(2): 377-382
    [29] 张鹏,霍燕,周淼平,姚金保,马鸿翔. 小麦禾谷镰孢菌茎基腐病抗源的筛选与评价. 植物遗传资源学报, 2009, 10(3): 431-435Zhang P, Huo Y, Zhou M P, Yao J B, Ma H X. Identification and evaluation of wheat germplasm resistance to crown rot caused by Fusarium graminearum. Journal of Plant Genetic Resources, 2009, 10(3): 431-435
    [30] Lv G G, Zhang Y X, Ma L, Yan X N, Yuan M J, Chen J H, Cheng Y Z, Yang X, Qiao Q, Zhang L L, Niaz M, Sun X N, Zhang Q J, Zhong S B, Chen F. A cell wall invertase modulates resistance to fusarium crown rot and sharp eyespot in common wheat. Journal of Integrative Plant Biology, 2023, 65(7):1814-1825
    [31] Flint-Garcia S A. Genetics and consequences of crop domestication. Journal of Agricultural and Food Chemistry, 2013, 61(35): 8267-8276
    [32] 陈浣,夏菲,吴新儒,孙玉合. 集群分离分析法在植物基因定位上的应用. 基因组学与应用生物学, 2016, 35(6): 1546-1551Chen H, Xia F, Wu X R, Sun Y H. The application of bulked segregant analysis in plant gene mapping. Genomics and Applied Biology, 2016, 35(6): 1546-1551
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

Copy
Share
Article Metrics
  • Abstract:330
  • PDF: 1147
  • HTML: 307
  • Cited by: 0
History
  • Received:July 18,2023
  • Revised:August 26,2023
  • Online: January 26,2024
  • Published: January 26,2024
Article QR Code
You are the 604580th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.