Abstract:Through cooperating with the fruit industry authorities of counties (districts) in Gannan (southern Jiangxi province, China) and visiting fruit farmers, we conducted the survey of wild Fortunella hindsii germplasm resources and collected 716 samples for phenotype characterization. The F. hindsii germplasm resources were found in Anyuan, Longnan, Zhanggong, Chongyi, Yudu, Xinfeng and other counties (districts) in Gannan, Jiangxi province, China. It can be divided into wild and artificial transplantation, and the main habitat is primeval forest, forest, valleys, hills and bonsai. There were phenotypic variations at 16 traits including tree posture, internode length, thorn number, thorn length, leaf length, leaf width, petiole, leaf shape, wing leaf, leaf tip lack of engraving, single fruit weight, fruit surface main-color, fruit surface variegation, seed number, oil cell obviousness, and peel thickness. Based on the phenotypic datasets, these samples were classified into four categories at Euclidean distance equal to 17.5, and further less than 14 into 8 subgroups. Principal component analysis was performed based on 16 traits identified 6 principal components with eigenvalues greater than 1 and associating with agronomic traits. To meet the need of citrus industry, 3 thornless, 34 seedless, 67 big-fruit and 12 late-ripening were selected. To sum up, the results of wild F. hindsii in Gannan have clear valuable for the commercial breeding of new F. hindsii varieties and the development of the citrus industry in future.