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Home > Archive>Volume 25, Issue 11, 2024 >1907-1922. DOI:10.13430/j.cnki.jpgr.20240217002
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Genome Wide Association Study of Rust Resistance in Cowpea
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Affiliation:

1.College of Horticulture Science， Zhejiang A&F University， Hangzhou 311300;2.Institute of Vegetable，Zhejiang Academy of Agricultural Sciences/Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China (Co-construction by Ministry and Province)， Ministry of Agriculture and Rural Affairs， Hangzhou 310021;3.General Station for Seed Administration of Zhejiang Province， Hangzhou 310020;4.Institute of Vegetable， Guangdong Academy of Agricultural Sciences， Guangzhou 510640;5.State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products， Zhejiang Academy of Agricultural Sciences， Hangzhou 310021

Fund Project:

Foundation projects： 2022-2024 Crop Germplasm Identification Project in Zhejiang Province （2023R23T60D01，2022R23T60D04）； National Natural Science Foundation of China （32172568）， Major Science and Technology Project of Plant Breeding in Zhejiang Province （2021C02065-6-3）； Key R&D Program of Shandong Province （2023LZGCQY012）

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    Abstract:

    Cowpea is one of the most important legume crops worldwide， serving not only as an important coarse cereal but also as a traditional vegetable in China. Rust is one of the three major diseases in cowpea， and developing rust resistance cultivars is the most cost-effective strategy in disease control. Exploring the resistant germplasm and elucidating the genetic basis of rust resistance is the key and prerequisite for cowpea rust resistance breeding. In the current study， 215 cowpea landraces were examined for rust resistance and 40 accessions showing resistance to rust were identified. Through resequencing the 215 accessions， 3880169 high-quality SNPs and 469398 high-quality InDels were found. These accessions were divided into four subgroups through population structural analysis， and the subgroup division was highly related to pod length and pod type. Total of 10 genomic regions， which significantly associated with rust resistance and distributed on seven chromosomes， were detected by GWAS. Haplotype analysis based on the associated SNPs showed that their alleles were highly correlated to rust resistance. The ratio of resistance accessions increased significantly， when pyramiding increasing number of favored alleles. Ten candidate genes were identified， including BTB/POZ domain-containing protein， LRR receptor-like serine/threonine-protein kinase， ethylene-responsive transcription factor and alternative oxidase genes. These results will provide the theoretical guidance and gene resources for molecular breeding of rust resistance in cowpea.

    Reference
    [1] Herniter I A, Mu?oz-Amatriaín M, Close T J. Genetic, textual, and archeological evidence of the historical global spread of cowpea ( Vigna unguiculata [L.] Walp. ). Legume Science, 2020, 2: 57
    [2] 中华人民共和国农业农村部. 2006年全国各地蔬菜播种面积和产量. 中国蔬菜, 2008(1): 65Ministry of Agriculture and Rural Affairs of the People’s Republic of China. The vegetables sowing areas and yield across the country in 2006. China Vegetables, 2008(1): 65
    [3] 中华人民共和国农业农村部. 2005年全国各地蔬菜播种面积和产量. 中国蔬菜, 2007(1): 40-41Ministry of Agriculture and Rural Affairs of the People’s Republic of China. The vegetables sowing areas and yield across the country in 2005. China Vegetables, 2007(1): 40-41
    [4] Barclay A. Additional Uredineae from the neighbourhood of Simla. Journal of the Asiatic Society of Bengal, 1891, 60: 211-230
    [5] Tagoe S M A, Mensah T A, Asare A T. Effect of rust (Uromyces Phaseoli Var. Vignae) infection on photosynthetic efficiency, growth and yield potentials of cowpea (Vigna Unguiculata L. Walp ) in an open field system. Global Journal of Science Frontier Research, 2020, 20(2): 49-61
    [6] 陈伟达, 刘琴, 陈禅友. 豇豆锈病研究进展. 江汉大学学报:自然科学版, 2016, 44(6): 509-513Chen W D, Liu Q, Chen C Y. Current status of cowpea rust research. Journal of Jianghan University: Natural Science Edition, 2016, 44(6): 509-513
    [7] Agrios G. Plant pathology. 5nd edn. Amsterdam: Elsevier Academic Press, 2004: 1-922
    [8] Deshpande S, Patil B R, Salimath P. Evaluation of native and collected germplasm for earliness seed traits and resistance to rust, cmv and leaf spot in cowpea [Vigna unguiculata (L.) Walp ]. Electronic Journal of Plant Breeding, 2010, 1: 384-392
    [9] Leonard K J, Szabo L J. Stem rust of small grains and grasses caused by Puccinia graminis. Molecular Plant Pathology, 2005, 6: 99-111
    [10] 张衍荣, 方木王, 黄健坤. 长豇豆锈病抗性遗传研究. 中国蔬菜, 1997(6): 10-12Zhang Y R, Fang M W, Huang J K. Study on inheritance of resistance to ruse in yardlongbean. China Vegetables, 1997(6): 10-12
    [11] Chen C Y,Heath M C. Inheritance of resistance to Uromyces vignae in cowpea and the correlation between resistance and sensitivity to a cultivar-specific elicitor of necrosis. Phytopathology, 1993, 83(2): 223-230
    [12] Ryerson D E, Heath M C. Inheritance of resistance to the cowpea rust fungus in cowpea cultivar Calico Crowder. Canadian Journal Plant Pathology, 1996, 18(4): 384-391
    [13] Uma M S, Hegde N, Hittalmani S. Identification of SSR marker associated with rust resistance in cowpea (Vigna unguiculata L.) using bulk segregant analysis. Legume Research, 1016, 39(1): 39-42
    [14] Wu X Y, Wang B G, Wu X H, Lu Z F, Li G J, Xu P. SNP marker-based genetic mapping of rust resistance gene in the vegetable cowpea landrace ZN016. Legume Research, 2018, 41(2): 222-225
    [15] Wu X Y, Li G J, Wang B G, Hu Y W, Wu X H, Wang Y, Lu Z F, Xu P. Fine mapping Ruv2, a new rust resistance gene in cowpea ( Vigna unguiculata ), to a 193-kb region enriched with NBS-type genes. Theoretical and Applied Genetics, 2018, 131(12): 2709-2718
    [16] Fiscus C J, Herniter I A, Tchamba M, Paliwal R, Mu?oz-Amatriaín M, Roberts P A, Abberton M, Alaba O, Close T J, Oyatomi O. The pattern of genetic variability in a core collection of 2021 cowpea accessions.G3, 2024,14(6):jkae071
    [17] 刘旭, 黎裕, 李立会, 贾继增. 作物种质资源学理论框架与发展战略. 植物资源遗传学报, 2023, 24(1): 1-10Liu X, Li Y, Li L H, Jia J Z. Theoretical framework and development strategy for the science of crop germplasm resources. Journal of Plant Genetic Resources, 2023, 24(1): 1-10
    [18] Ma Z, Xie Q, Li G, Jia H, Zhou J, Kong Z, Li N, Yuan Y. Germplasms, genetics and genomics for better control of disastrous wheat Fusarium head blight. Theoretical and Applied Genetics, 2020, 133(5): 1541-1568
    [19] Mitchum M G. Soybean resistance to the soybean cyst nematode Heterodera glycines: An update. Phytopathology, 2016, 106: 1444-1445
    [20] Wu X Y, Wu X H, Xu P, Wang B G, Lu Z F, Li G J. Association mapping for fusarium wilt resistance in Chinese asparagus bean germplasm. Plant Genome, 2015, 8 (2): eplantgenome 2014.11.0082.
    [21] Heng T, Kaga A, Chen X, Somta P. Two tightly linked genes coding for NAD-dependent malic enzyme and dynamin-related protein are associated with resistance to Cercospora leaf spot disease in cowpea (Vigna unguiculata (L.) Walp.). Theoretical and Applied Gentics, 2020, 133(2): 395-407
    [22] Pan L, Liu M, Kang Y, Mei X, Hu G, Bao C, Zheng Y, Zhao H, Chen C, Wang N. Comprehensive genomic analyses of Vigna unguiculata provide insights into population differentiation and the genetic basis of key agricultural traits. Plant Biotechnol Journal, 2023, 21(7): 1426-1439
    [23] Lonardi S, Mu?oz-Amatriaín M, Liang Q, Shu S, Wanamaker SI, Lo S, Tanskanen J, Schulman A H, Zhu T, Luo M C, Alhakami H, Ounit R, Hasan A M, Verdier J, Roberts P A, Santos J R P, Ndeve A, Dole?el J, Vrána J, Hokin S A, Farmer A D, Cannon S B, Close T J. The genome of cowpea (Vigna unguiculata [L.] Walp.). The Plant Journal, 2019, 98(5): 767-782
    [24] Yang Y, Wu Z, Wu Z, Li T, Shen Z, Zhou X, Wu X, Li G, Zhang Y. A near-complete assembly of asparagus bean provides insights into anthocyanin accumulation in pods. Plant Biotechnol Journal, 2023, 21(12): 2473-2489
    [25] Mu?oz-Amatriaín M, Mirebrahim H, Xu P, Wanamaker S I, Luo M, Alhakami H, Alpert M, Atokple I, Batieno B J, Boukar O, Bozdag S, Cisse N, Drabo I, Ehlers J D, Farmer A, Fatokun C, Gu Y Q, Guo Y N, Huynh B L, Jackson S A, Kusi F, Lawley C T, Lucas M R, Ma Y, Timko M P, Wu J, You F, Barkley N A, Roberts P A, Lonardi S, Close T J. Genome resources for climate-resilient cowpea, an essential crop for food security. The Plant Journal, 2017, 89(5): 1042-1054
    [26] Kawashima C G, Guimar?es G A, Nogueira S R, MacLean D, Cook D R, Steuernagel B, Baek J, Bouyioukos C, Melo Bdo V, Trist?o G, de Oliveira J C, Rauscher G, Mittal S, Panichelli L, Bacot K, Johnson E, Iyer G, Tabor G, Wulff B B, Ward E, Rairdan G J, Broglie K E, Wu G, van Esse H P, Jones J D, Brommonschenkel S H. A pigeonpea gene confers resistance to Asian soybean rust in soybean. Nature Biotechnology, 2016, 34(6): 661-665
    [27] Periyannan S, Moore J, Ayliffe M, Bansal U, Wang X, Huang L, Deal K, Luo M, Kong X, Bariana H, Mago R, McIntosh R, Dodds P, Dvorak J, Lagudah E. The gene Sr33, an ortholog of barley Mla genes, encodes resistance to wheat stem rust race Ug99. Science, 2013, 341(6147): 786-788
    [28] Saintenac C, Zhang W, Salcedo A, Rouse M N, Trick H N, Akhunov E, Dubcovsky J. Identification of wheat gene Sr35 that confers resistance to Ug99 stem rust race group. Science, 2013, 341(6147): 783-786
    [29] Mago R, Zhang P, Vautrin S, ?imková H, Bansal U, Luo M C, Rouse M, Karaoglu H, Periyannan S, Kolmer J, Jin Y, Ayliffe M A, Bariana H, Park R F, McIntosh R, Dole?el J, Bergès H, Spielmeyer W, Lagudah E S, Ellis J G, Dodds P N. The wheat Sr50 gene reveals rich diversity at a cereal disease resistance locus. Nature Plants, 2015, 1: 15186
    [30] Brueggeman R, Rostoks N, Kudrna D, Kilian A, Han F, Chen J, Druka A, Steffenson B, Kleinhofs A. The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99 (14): 9328-9333
    [31] Krattinger S G, Lagudah E S, Spielmeyer W, Singh R P, Huerta-Espino J, McFadden H, Bossolini E, Selter L L, Keller B. A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science, 2009, 323(5919): 1360-1363
    [32] Moore J W, Herrera-Foessel S, Lan C, Schnippenkoetter W, Ayliffe M, Huerta-Espino J, Lillemo M, Viccars L, Milne R, Periyannan S, Kong X, Spielmeyer W, Talbot M, Bariana H, Patrick J W, Dodds P, Singh R, Lagudah E. A recently evolved hexose transporter variant confers resistance to multiple pathogens in wheat. Nature Genetics, 2015, 47(12): 1494-1498
    [33] Zhao M, Ge Y, Xu Z, Ouyang X, Jia Y, Liu J, Zhang M, An Y. A BTB/POZ domain-containing protein negatively regulates plant immunity in Nicotiana benthamiana. Biochemical and Biophysical Research Communications, 2022, 600: 54-59
    [34] Saha B, Borovskii G, Panda S K. Alternative oxidase and plant stress tolerance. Plant Signaling ＆ Behavior, 2016, 11 (12): e1256530
    [35] Licausi F, Ohme-Takagi M, Perata P. APETALA2/Ethylene Responsive Factor (AP2/ERF) transcription factors: Mediators of stress responses and developmental programs. New Phytologist, 2013, 199: 639-649
    [36] Lease K A, Lau N Y, Schuster R A, Torii K U, Walker J C. Receptor serine/threonine protein kinases in signalling: Analysis of the erecta receptor-like kinase of Arabidopsis thaliana. New Phytologist, 2001, 151(1): 133-143
    [37] Du Y, Shi Y, Yang J, Chen X, Xue M, Zhou W, Peng Y L. A serine/threonine-protein phosphatase PP2A catalytic subunit is essential for asexual development and plant infection in Magnaporthe oryzae. Current Genetics, 2013, 59(1-2): 33-41
    [38] Bishop J G, Ripoll D R, Bashir S, Damasceno C M, Seeds J D, Rose J K. Selection on glycine beta-1,3-endoglucanase genes differentially inhibited by a Phytophthora glucanase inhibitor protein. Genetics, 2005, 169(2): 1009-1019
    [39] Ruano G, Scheuring D. Plant cells under attack: Unconventional endomembrane trafficking during plant defense. Plants (Basel), 2020, 9(3): 389
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  • Received:February 17,2024
  • Online: November 07,2024
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