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Home > Archive>Volume 25, Issue 11, 2024 >1907-1922. DOI:10.13430/j.cnki.jpgr.20240217002
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Genome Wide Association Study of Rust Resistance in Cowpea
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Affiliation:

1.College of Horticulture Science， Zhejiang A&F University， Hangzhou 311300;2.Institute of Vegetable，Zhejiang Academy of Agricultural Sciences/Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China (Co-construction by Ministry and Province)， Ministry of Agriculture and Rural Affairs， Hangzhou 310021;3.General Station for Seed Administration of Zhejiang Province， Hangzhou 310020;4.Institute of Vegetable， Guangdong Academy of Agricultural Sciences， Guangzhou 510640;5.State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products， Zhejiang Academy of Agricultural Sciences， Hangzhou 310021

Fund Project:

Foundation projects： 2022-2024 Crop Germplasm Identification Project in Zhejiang Province （2023R23T60D01，2022R23T60D04）； National Natural Science Foundation of China （32172568）， Major Science and Technology Project of Plant Breeding in Zhejiang Province （2021C02065-6-3）； Key R&D Program of Shandong Province （2023LZGCQY012）

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    Abstract:

    Cowpea is one of the most important legume crops worldwide， serving not only as an important coarse cereal but also as a traditional vegetable in China. Rust is one of the three major diseases in cowpea， and developing rust resistance cultivars is the most cost-effective strategy in disease control. Exploring the resistant germplasm and elucidating the genetic basis of rust resistance is the key and prerequisite for cowpea rust resistance breeding. In the current study， 215 cowpea landraces were examined for rust resistance and 40 accessions showing resistance to rust were identified. Through resequencing the 215 accessions， 3880169 high-quality SNPs and 469398 high-quality InDels were found. These accessions were divided into four subgroups through population structural analysis， and the subgroup division was highly related to pod length and pod type. Total of 10 genomic regions， which significantly associated with rust resistance and distributed on seven chromosomes， were detected by GWAS. Haplotype analysis based on the associated SNPs showed that their alleles were highly correlated to rust resistance. The ratio of resistance accessions increased significantly， when pyramiding increasing number of favored alleles. Ten candidate genes were identified， including BTB/POZ domain-containing protein， LRR receptor-like serine/threonine-protein kinase， ethylene-responsive transcription factor and alternative oxidase genes. These results will provide the theoretical guidance and gene resources for molecular breeding of rust resistance in cowpea.

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  • Received:February 17,2024
  • Online: November 07,2024
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