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Home > Archive>Volume 25, Issue 11, 2024 >1955-1966. DOI:10.13430/j.cnki.jpgr.20240219003
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Cloning and Preliminary Functional Analysis of Salt Tolerance Gene FtGST2 in Tartary Buckwheat
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Affiliation:

1.College of Agriculture，Shanxi Agricultural University， Jinzhong 030801;2.Institute of Crop Sciences， Chinese Academy of Agricultural Sciences/ Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization(MARA)，Beijing 100081;3.Shanxi Provincial Key Laboratory of Germplasm Innovation and Molecular Breeding of Coarse Grains， Jinzhong 030801

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Foundation project： National Natural Science Foundation of China（32161143005）

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    Abstract:

    In the previous study， a salt tolerance related gene FtGST2 （FtPinG0707941400.01） in tartary buckwheat was identified through the whole genome association analysis. The homologous alignment and conserved sequence analysis revealed that the gene belongs to the tetrachlorohydroquinone dehalogenase subfamily of glutathione-S-transferases. We cloned the FtGST2 gene from the cultivar ‘Zhongku-3’， and identified 34 responsive elements including 2 abscisic acid responsive elements and 6 methyl jasmonate responsive elements， in the 2000 bp upstream promoter sequence. Abscisic acid and jasmonic acid are important stimuli for regulating salt stress pathways in plants. Through analyzing the expression levels of FtGST2 in different organs of tartary buckwheat and the differences in expression levels under different concentrations of NaCl treatment， the highest transcripts of FtGST2 were detected in roots， and also under 100 mmol/L NaCl treatment. In addition， we over-expressed FtGST2 in Arabidopsis thaliana and hairy roots， and detected the seed germination rate， main root length， fresh weight， and physiological indicators. The germination rate and root length of Arabidopsis thaliana overexpressing FtGST2 under salt treatment were higher than those of wild-type. The fresh weight of hairy roots overexpressing FtGST2 under salt treatment was significantly higher than that of the control group A4 hairy roots. After testing the four physiological indicators of SOD， POD， CAT， and MDA in hairy roots， it was found that overexpression of FtGST2 gene can effectively improve the salt tolerance of tartary buckwheat hairy root overexpression strains. Collectively， these experiments provided preliminary evidence on the salt resistance function of the FtGST2 gene， laying a certain foundation for the breeding of salt tolerant tartary buckwheat varieties in the future.

    Reference
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  • Received:February 19,2024
  • Online: November 07,2024
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