The rice variety Shaoxiang100 with low cadmium accumulation was developed through heavy-ion mutagenesis of the aromatic high-quality rice material 44-5， through applying the M₁TDS technology and the mutation of the OsNRAMP5 gene. The variety Shaoxiang100 underwent multi-location testing in Hunan province from 2021 to 2023， and has also been part of national independent trials for varieties with low cadmium （Cd） accumulation. The mutation of the OsNRAMP5 gene reduces Cd accumulation， and also manganese （Mn）， which is essential for plant growth. This study investigated the Cd accumulation characteristics， yield， quality， and low-temperature tolerance of Shaoxiang100 and its original strain 44-5. These multi-location experiments revealed that Shaoxiang 100 exhibited stable， low levels of Cd in rice grains， with a reduced accumulation of Mn in plants. However， no significant difference was observed in yield when compared to the original variety， 44-5. For main agronomic traits， except for plant height， no significant difference was detected. Both Shaoxiang 100 and the original line 44-5 exhibited a significant decrease in fruiting rate when delay sowing applied. However， late sowing didn’t affect yield or other main agronomic traits， except for plant height.Additionally， the relative cold tolerance coefficient and variety cold tolerance index were 0.75 and 0.75， respectively， indicating a decrease of 47.2% and 49.2%. The low-temperature tolerance at the spikelet stage was classified as level 4， indicating sensitivity to cold. Compared to the original strain 44-5， Shaoxiang 100 did not show a decrease in Mn accumulation or low-temperature tolerance. Both strains were found to have general cold tolerance. Collectively， it is recommended that Shaoxiang 100 as double-crop late rice， can be planted in Hunan and Jiangxi to prevent yield loss from low-temperature stress during the late growth period.