Evaluation of Lodging Resistance and Selection of Identification Indexes of Common Buckwheat Germplasm Resources
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1.Dingxi Academy of Agricultural Sciences, Dingxi 743000,Gansu;2.Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070;3.Fengxiang Town Government, Anding District, Dingxi 743000,Gansu;4.College of Food and Biological Engineering, Chengdu University, Chengdu 610106

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Science and Technology Program of Gansu Province (24CXNJ014); The Opening Project of Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, and Sichuan Engineering and Technology Research Center of Coarse Cereal Industralization, Chengdu University(2023CC002); National Oats and Buckwheat Industry Technology System Program (CARS-07-G-12)

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    Abstract:

    Lodging is a main problem affecting the yield of common buckwheat in central Gansu, China. This study used 76 common buckwheat germplasm resources from both domestic and international sources to investigate genetic diversity of 23 phenotypic traits that associate to lodging resistance. Through applying statistic methods such as Shannon-Wiener diversity index, correlation analysis, principal component analysis, regression analysis, cluster analysis and stepwise discriminate analysis, a comprehensive evaluation of the lodging resistance was conducted. The collection of common buckwheat exhibited high genetic diversity, with diversity indices ranging from 2.349 to 4.331 for different traits, among which the number of main stem branches was the smallest and the length of the second inter-node was the highest. Correlation analysis revealed correlations of varying degrees among the 23 traits. The principal component analysis showed that the first five principal components could represent 81.421% of the information of the 23 traits. Using a membership function method, a comprehensive score value (D) for lodging resistance was calculated, with an average D value of 0.469. The D value for Yuntianqiao 1 was the lowest (0.200) and the highest for TQ10-07(0.819). Stepwise regression analysis identified eight traits, including plant height, stem height of center gravity, the first inter-node length and wall thickness, the second inter-node length, number of main stem branches, bending degree and stem resistance, as comprehensive evaluation indicators for lodging resistance of common buckwheat germplasm resources. Based on D value clustering, 76 materials were divided into four groups,among of which, 10 materials in class Ⅳ exhibited high lodging resistance and high D value, which could be used as parent materials for material innovation and breeding. This study showed that using a multivariate statistical analysis method for comprehensive evaluation of lodging resistance is feasible, providing a basis and reference for germplasm innovation and lodging-resistant breeding in common buckwheat in central Gansu, China.

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History
  • Received:March 14,2024
  • Revised:
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  • Online: January 07,2025
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