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Home > Archive>Volume 25, Issue 12, 2024 >2069-2080. DOI:10.13430/j.cnki.jpgr.20240319002
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Evaluation of Cross Resistance to ALS Herbicides and Expression Analysis of Resistance Gene BnAHAS1-3R in Rapeseed M196
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Affiliation:

1.College of Life Sciences， Jiangsu University， Zhenjiang 212023;2.Institute of Industrial Crops， Jiangsu Academy of Agricultural Sciences/Nanjing Sub-center， National Center of Oil Crops Improvement/Key Laboratory of Cotton and Rapeseed (Nanjing)， Ministry of Agriculture and Rural Affairs/Jiangsu Key Laboratory for Agro-Biology， Nanjing 210014;3.College of Agriculture， Fujian Agriculture and Forestry University， Fuzhou 350002;4.College of Agriculture， Nanjing Agricultural University， Nanjing 210095

Fund Project:

Foundation projects： Science and Technology Innovation 2030—Major Program （2023ZD0404203）；Youth Fund of the National Natural Science Foundation of China（31901503）；Jiangsu Province Agricultural Science and Technology Independent Innovation Fund Project（CX（23）1001-1）；Major Independent Research Project of Jiangsu Provincial Key Laboratory of Agricultural Biology（JKLA2021-ZD02）；National Agricultural Modern Industrial Technology System Construction Project（CARS-12）；Jiangsu Province Key Research and Development Project（BE2021405）

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    Abstract:

    In order to identify effects of the cross-resistance and the function of resistant genes BnAHAS1-3R in germplasm M196， this study examined the resistance effect of rapeseed M196 and N131 （wild type） by spraying sulfonylurea， sulfonlyaminocarbonyl-triazolinone， imidazolinone， triazolopyrimidine， and pyrimidyl-benzoate with different concentration gradients （0， 1/4， 1/2， 1， 2 and 4 times the recommended concentration of herbicides in the field）， respectively， at 3-5 leaf stage. Based on the greenhouse and field results， the cross-resistance of SU and SCT was observed simultaneously to be the strongest， while IMI， TP and PB were lower. Results of qRT-PCR and in vitro enzyme inhibition experiments in transgenic Arabidopsis indicated that the cross-resistance of ALS herbicides was caused by the elevated expression of the mutated allele of BnAHAS1-3R and the decrease on enzyme sensitivity to herbicides. The study provides a theoretical basis for preventing and resolving the potential problems about weed resistance and drift of resistance gene that may arise from the widespread promotion of resistant varieties.

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History
  • Received:March 19,2024
  • Online: December 06,2024
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