Evaluation of Salt Tolerance and Analysis of Heterosis Patterns in Maize during Germination
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1.College of Agronomy, Jilin Agricultural University;2.Institute of Botany, Chinese Academy of Sciences;3.Institute of Crop Science, Chinese Academy of Agricultural Sciences

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National key research and development program of China

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    Abstract:

    Abstract: To evaluate the tolerance of different maize materials to salt stress during the germination period and explore the heterozygous patterns of salt tolerance in maize hybrid materials during the germination period. This study used a 160 mmol/L NaCl solution to treat 152 maize inbred lines and 303 hybrid varieties under salt stress. Distilled water culture was used as the control, and salt tolerance during germination was identified using a paper bed culture method in a culture dish. The results showed that salt stress treatment significantly reduced the germination potential, germination rate, and germination index of maize materials. Trait correlation analysis revealed a highly significant positive correlation between different traits. Using the salt tolerance rate during the germination period and combining with the clustering analysis results, the inbred and hybrid materials were divided into five salt tolerance levels: Level I (high tolerance), Level II (tolerance), Level III (moderate), Level IV (sensitive), and Level V (high sensitivity). According to the results of IBD (identity by descent) analysis, 146 inbred line materials were divided into 8 groups. The salt tolerance of different groups of inbred line materials was as follows: EUROM group>Complex group>ZI330 group>PB group>LAN group>SPT group>PA group>REID group. The hybrid materials are divided into 23 heterosis patterns based on the different parent material groups, among which EUROM × PB is the optimal heterosis pattern for salt stress tolerance during germination. This study identified the salt tolerance of inbred lines and hybrid materials, and selected 4 high salt tolerant inbred line materials and 17 high salt tolerant hybrid materials; It has been preliminarily determined that the EUROM group inbred lines have strong salt tolerance, and the optimal heterosis pattern is EUROM × PB.

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History
  • Received:April 05,2024
  • Revised:April 26,2024
  • Adopted:September 19,2024
  • Online: November 20,2024
  • Published:
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