Genetic Analysis of Powdery Mildew Resistance in Siyuehuang, a Wheat Landrace in Shanxi Province, China
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Affiliation:

1.Jinzhong Agricultural Seed Industry Breeding and Improvement Station,Jinzhong 030600,Shanxi;2.College of Agricultural, Shanxi Agricultural University, Taiyuan 030031

Fund Project:

Foundation projects: National Key R&D Program of China(2022YFD1200901); Shanxi Province Wheat Breeding Joint Research(NYGG26); Shanxi Agricultural University Science and Technology Innovation Enhancement Project(CXGC202305); Breeding Engineering Project of College of Agriculture, Shanxi Agricultural University(YZ2021-0)

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    Abstract:

    Powdery mildew is a devastating fungal pathogen that threatens wheat production. Identifying resistant germplasm and exploring resistance genes are crucial for sustainable improvement of wheat genetic resistance. Siyuehuang, a wheat landrace identified with resistance to powdery mildew from Shanxi Province’s wheat germplasm resources, was crossed with susceptible cultivar Jinmai 47. The segregation population was developed and subjected for artificial inoculation for resistance assessment and genetic analysis at seedling stage. We employed the bulked segregant analysis (BSA) combined with a 90K SNP array to locate the resistance genes. The resistance of Siyuehuang to powdery mildew was controlled by a single dominant nuclear gene (provisionally named PmSYH), with an inheritance pattern in accordance with Mendelian inheritance law. SNP array analysis revealed that polymorphic SNP markers were mainly enriched in the 110 Mb to 140 Mb segment on the short arm of wheat chromosome 7D. SSR (Simple Sequence Repeats) molecular markers were developed in the interval, and six markers linked to PmSYH were obtained for genotyping the F2 population. PmSYH was mapped in the physical interval of 132.6 Mb to 137.5 Mb, flanked by Sxau7DS-37 and Sxau7DS-48 with genetic distance of 1.8 cM and 3.1 cM, respectively. Comparative analysis with previous localization results suggests that PmSYH is a new resistant gene against powdery mildew. This study provided wheat new genetic resources for wheat resistance breeding, with a certain value for improving resistance to powdery mildew in the northern winter wheat region.

    Reference
    [1] Chaves M S,Martinelli J A,Wesp-Guterres C,Graichen F A S,Bram-mer S P,Scagliusi S M,da Silva P R,Wieth?lter P,Torres G A M,Lau E Y,Luciano C,Ana L S C. The importance for food security of maintaining rust resistance in wheat. Food Security,2013,5:157-176
    [2] Zhai H Q,Cao S Q,Wan J M,Zhang R X,Lu W,Li L B,Kuang T Y,Min S K,Zhu D F,Cheng S H. Relationship between leaf photosynthetic function at grain filling stage and yield in super high-yielding hybrid rice (Oryza sativa. L). Science China(Life Sciences),2002,45(6):637-646
    [3] Kumar Y,Mishra S K,Tyagi M C,Singh S P,Sharma B. Linkage between genes for leaf colour,plant pubescence,number of leaf-lets and plant height in lentil (Lens culinaris Medik.). Euphytica,2005,145:41-48
    [4] Wang Z H,Wang Y,Hong X,Hu D H,Liu C X,Yang J,Li Y,Huang Y Q,Feng Y Q,Gong H Y,Li Y,Fang G,Tang H R,Li Y S. Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice. Journal of Experimental Botany,2014,66(3):973-987
    [5] 陈芳,乔麟轶,李锐,刘成,李欣,郭慧娟,张树伟,常利芳,李东方,阎晓涛,任永康,张晓军,畅志坚. 小麦新种质CH1357抗白粉病遗传分析及染色体定位. 作物学报,2019,45(10):1503-1510Chen F,Qiao L Y,Li R,Liu C,Li X,Guo H J,Zhang S W,Chang L F,Li D F,Yan X T,Ren Y K,Zhang X J,Chang Z J. Genetic analysis and chromosomal localization of powdery mildew resistance gene in wheat germplasm CH1357. Acta Agronomica Sinica,2019,45(10):1503-1510
    [6] 原宗英,武英鹏,夏宏,张治家,夏青. 山西小麦品种和育种材料抗锈病、白粉病鉴定. 中国植保导刊,2017,37(2):15-18Yuan Z Y,Wu Y P,Xia H,Zhang Z J,Xia Q. Evaluation of Shanxi wheat cultivars for resistance to wheat stripe rust,leaf rust and powdery mildew. China Plant Protection,2017,37(2):15-18
    [7] 黄冲,姜玉英,李春广. 1987年-2018年我国小麦主要病虫害发生危害及演变分析. 植物保护,2020,46(6):186-193Huang C,Jiang Y Y,Li C G. Occurrence,yield loss and dynamics of wheat diseases and insect pests in China from 1987 to 2018. Plant Protection,2020,46(6):186-193
    [8] 刘敏捷,原宗英,李霞,武英鹏,周建波. 不同杀菌剂对小麦白粉病的田间防效. 中国植保导刊,2019,39(6):70-71Liu M J,Yuan Z Y,Li X,Wu Y P,Zhou J B. Field control effect of different fungicides on wheat powdery mildew. China Plant Protection,2019,39(6):70-71
    [9] 郎漫,李平,蔡祖聪. 百菌清在土壤中的降解及其生态环境效应. 中国农学通报,2012,25(15):211-215Lang M,Li P,Cai Z C. The degradation of chlorothalonil in soil and its environmental implications. Chinese Agricultural Science Bulletin,2012,25(15):211-215
    [10] 吴文铸,郭敏,孔德洋,许静,单正军. 3种三唑类杀菌剂的环境降解特性. 生态与农村环境学报,2016,32(5):837-841Wu W T,Guo M,Kong D Y,Xu J,Shan Z J. Environmental degradation properties of 3 triazole fungicides. Journal of Ecology and Rural Environment,2016,32(5):837-841
    [11] 李菊颖,严伟强,何健,吴文铸,孔德洋,单正军. 戊唑醇在环境中的降解迁移和生物富集性研究. 生态毒理学报,2017,12(4):310-318Li J Y,Yan W Q,He J,Wu W Z,Kong D Y,Shan Z J. Degradation,migration and bioaccumulation of tebuconazole in the environment. Asian Journal of Ecotoxicology,2017,12(4):310-318
    [12] 杨作民,唐伯让,沈克全,夏先春. 小麦抗病育种的战略问题:小麦对锈病和白粉病第二线抗源的建立和利用. 作物学报,1994,20(4):385-394Yang Z M,Tang B R,Shen K Q,Xia X C. Strategic issues in wheat disease resistance breeding:The establishment and utilization of wheat second-line resistance to rust and powdery mildew. Acta Agronomica Sinica,1994,20(4):385-394
    [13] Wang W R,He H G,Gao H M,Xu H X,Song W Y,Zhang X,Zhang L P,Song J C,Liu C,Liu K C,Ma P T. Characterization of the powdery mildew resistance gene in wheat breeding line KN0816 and its evaluation in marker-assisted selection. Plant Disease,2021,105(12):4042-4050
    [14] Golzar H,Shankar M,D′Antuono M. Responses of commercial wheat varieties and differential lines to western Australian powdery mildew (Blumeria graminis f. Sp. tritici) populations. Australasian Plant Pathology ,2016,45 (4):347-355
    [15] Parks R,Carbone I,Murphy J P,Marshall D,Cowger C. Virulence structure of the Eastern U.S. wheat powdery mildew population. Plant Disease,2008,92(7):1074-1082
    [16] Singh R P,Singh P K,Rutkoski J,Hodson D P,He X,J?rgensen L N,Hovm?ller M S,Espino J H. Disease Impact on wheat yield potential and prospects of genetic control. Annual Review of Phytopathology,2016,54:303-322
    [17] Cowger C,Mehra L,Arellano C,Meyers E,Murphy J P. Virulence differences in blumeria graminis f. sp. tritici from the central and Eastern United States. Phytopathology,2018,108(3):402-411
    [18] 董策,张希太,蔺桂芬,肖磊,李炎艳,谢淑芹,裴艳婷,路其祥. 2013-2021年河北省审冬小麦品种抗病性分析. 山西农业科学,2023,51(3):306-312Dong C,Zhang X T,Lin G F,Xiao L,Li Y Y,Xie S Q,Pei Y T,Lu Q X. Disease resistance analysis of the registered wheat varieties in Hebei province from 2013 to 2021. Journal of Shanxi Agricultural Sciences,2023,51(3):306-312
    [19] Chen F,Jia H Y,Zhang X J,Qiao L Y,Li X,Zheng J,Guo H J,Powers C,Yan L L,Chang Z J. Positional cloning of PmCH1357 reveals the origin and allelic variation of the Pm2 gene for powdery mildew resistance in wheat. The Crop Journal,2019,7(6):771-783
    [20] 郭慧娟,贾举庆,李欣,乔麟轶,阎晓涛,任永康,常利芳,张树伟,畅志坚,张晓军. 小麦种质CH7015中抗白粉病基因的SSR定位. 华北农学报,2019,34(6):203-208Guo H J,Jia J Q,Li X,Qiao L Y,Yan X T,Ren Y K,Chang L F,Zhang S W,Chang Z J,Zhang X J. Mapping of resistance gene to powdery mildew in wheat cultivar CH7015 using SSR markers. Acta Agriculturae Boreali-Sinica,2019,34(6):203-208
    [21] 盛宝钦. 用反应型记载小麦苗期白粉病. 植物保护,1988,14(1):49Sheng B Q. Powdery mildew at seedling stage of wheat was recorded by reaction type. Plant Protection,1988,14(1):49
    [22] 李洪杰,王晓鸣,宋凤景,伍翠平,武小菲,张宁,周阳,张学勇. 中国小麦品种对白粉病的抗性反应与抗病基因检测.作物学报,2011,37(6):943-954Li H J,Wang X M,Song F J,Wu C P,Wu X F,Zhang N,Zhou Y,Zhang X Y. Response to powdery mildew and detection of resistance genes in wheat cultivars from China. Acta Agronomica Sinica,2011,37(6):943-954
    [23] 陈昆松,李方,徐昌杰,张上隆,傅承新. 改良CTAB法用于多年生植物组织基因组DNA的大量提取. 遗传,2004,26(4):529-531Chen K S,Li F,Xu C J,Zhang S L,Fu C X. An efficient macro-method of genomic DNA isolation from actinidia chinensis leaves. Hereditas,2004,26(4):529-531
    [24] 杨文静,乔麟轶,李欣,郭慧娟,陈芳,张树伟,常利芳,贾举庆,畅志坚,张晓军. 小麦抗条锈基因Yr69的连锁标记开发. 植物科学学报,2022,40(2):197-204Yang W J,Qiao L Y,Li X,Guo H J,Chen F,Zhang S W,Chang L F,Jia J Q,Chang Z J,Zhang X J. Development of linkage markers for stripe rust resistance gene Yr69 in triticum aestivum. Plant Science Journal,2022,40(2):197-204
    [25] Wang S C,Wong D,Forrest K,Allen A,Chao S,Huang B E,Maccaferri M,Salvi S,Milner S G,Cattivelli L,Mastrangelo A M,Whan A,Stephen S,Barker G,Wieseke R,Plieske J,International Wheat Genome Sequencing Consortium,Lillemo M,Mather D,Appels R,Dolferus R,Brown G G,Korol A,Akhunova A R,Feuillet C,Salse J,Morgante M,Pozniak C,Luo M C,Dvorak J,Morell M,Dubcovsky J,Ganal M,Tuberosa R,Lawley C,Mikoulitch I,Cavanagh C,Edwards K J,Hayden M,Akhunov E,Characterization of polyploid wheat genomic diversity using a high-density 90000 single nucleotide polymorphism array. Plant Biotechnology Journal,2014,12(6):787-796
    [26] 张晓军,杨文静,畅志坚,常利芳,闫贵云,张树伟,李欣,乔麟轶,郭慧娟,雷梦林,贾举庆,穆志新. 小麦抗条锈病基因Yr69连锁标记在育种中的应用评价. 麦类作物学报,2021,41(4):417-423Zhang X J,Yang W J,Chang Z J,Chang L F,Yan G Y,Zhang S W,Li X,Qiao L Y,Guo H J,Lei M L,Jia J Q,Mu Z X. Evaluation on the application of molecular markers linked with the wheat stripe rust resistance gene Yr69 in wheat breeding. Journal of Triticeae Crops,2021,41(4):417-423
    [27] 王运斌,江良荣,黄荣裕,黄育民,郑景生. 一种高效省本的非变性聚丙烯酰胺凝胶电泳银染法的建立-以水稻为例. 福建稻麦科技,2015,33(3):4-8Wang Y B,Jiang L R,Huang R Y,Huang Y M,Zheng J S. A high-efficiency and low cost method of DNA silver staining in non-denaturant polycrylamide gel electrophoresis-an example based on rice. Fujian Rice and Wheat Technology,2015,33(3):4-8
    [28] He H G,Liu R K,Ma P T,Du H N,Zhang H H,Wu Q H,Yang L J,Gong S J,Liu T L,Huo N X,Gu Y Q,Zhu S Y. Characterization of Pm68,a new powdery mildew resistance gene on chromosome 2BS of Greek durum wheat TRI 1796. Theoretical and Applied Genetics,2021,134(1):53-62
    [29] Li Y H,Wei Z Z,Sela H,Govta L,Klymiuk V,Roychowdhury R,Chawla H S,Ens J,Wiebe K,Bocharova V,Ben-David R,Pawar P B,Zhang Y Q,Jaiwar S,Molnár I,Dole?el J,Coaker G,Pozniak G J,Fahima T. Dissection of a rapidly evolving wheat resistance gene cluster by long-read genome sequencing accelerated the cloning of Pm69. Plant Communications,2023,5(1):100646
    [30] McIntosh R A,Dubcovsky J,RogersW J,XiaX C,Raupp W J. Catalogue of gene symbols for wheat:2020 supplement. Annual Wheat Newsletterl,2020,66:116-117
    [31] Lillemo M,Asalf B,Singh R P,Huerta E J,Chen X M,He Z H,Bjornstad A. The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theoretical and Applied Genetics,2008,116:1155-1166
    [32] Maxwell J J,Lyerly J H,Srnic G,Murphy J P,Cowger C,Parks R,Marshall D,Brown-Guedira G,Miranda L. A novel-derived powdery mildew resistance gene identified in common wheat. Crop Science,2012,52(3):1162-1170
    [33] Reddy I N B L,Chandrasekhar K,Zewdu Y,Dinoor A,Keller B,Bendavid R. Identification and genetic mapping of PmAF7DS a powdery mildew resistance gene in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics,2016,129:1127-1137
    [34] Tosa Y,Sakai K. The genetics of resistance of hexaploid wheat to the wheat grass powdery mildew fungus. Genome,1990,33(2):225-230
    [35] 杨立军,曾凡松,龚双军,史文琦,张学江,汪华,向礼波,喻大昭. 68个主推小麦品种的白粉病抗性分析及基因推导. 中国农业科学,2013,46(16):3354-3368Yang L J,Zeng F S,Gong S J,Shi W Q,Zhang X J,Wang H,Xiang L B,Yu D Z. Evaluation of resistance to powdery mildew in 68 Chinese major wheat cultivars and postulation of their resistance genes. Scientia Agricultura Sinica,2013,46(16):3354-3368
    [36] McIntosh R A,Zhang P,Cowger C,Parks R,Lagudah E S,Hoxha S. Rye-derived powdery mildew resistance gene Pm8 in wheat is suppressed by the Pm3 locus. Theoretical and Applied Genetics,2011,123(3):359-367
    [37] Qi L L,Cao M S,Chen P D,Li W L,Liu D J. Identification,mapping,and application of polymorphic DNA associated with resistance gene Pm21 of wheat. Genome,1996,39(1):191-197
    [38] 曹廷杰,陈永兴,李丹,张艳,王西成,赵虹,刘志勇. 河南小麦新育成品种(系)白粉病抗性鉴定与分子标记检测. 作物学报,2015,41(8):1172-1182Cao T J,Chen Y X,Li D,Zhang Y,Wang X C,Zhao H,Liu Z Y. Identification and molecular detection of powdery mildew resistance of new bred wheat varieties (lines) in Henan province,China. Acta Agronomica Sinica,2015,41(8):1172-1182
    [39] 杜习军. 河南省小麦抗白粉病种质资源筛选及优异基因发掘. 杨凌:西北农林科技大学,2021Du X J. Powdery mildew resistant germplasms screening and excellent genes discovery in wheat (Triticum aestivum L.) of Henan province. Yangling:Northwest A&F University,2021
    [40] 李静婷,刘子记.中国小麦地方品种抗白粉病基因的发掘与定位. 湖北农业科学,2015,54(17):4113-4116Li J T,Liu Z J. Discovery and mapping of powdery mildew resistance gene in wheat landraces in China. Hubei Agricultural Sciences,2015,54(17):4113-4116
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  • Received:April 26,2024
  • Online: January 23,2025
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