Genetic Analysis of Powdery Mildew Resistance in Siyuehuang， a Wheat Landrace in Shanxi Province， China

doi:10.13430/j.cnki.jpgr.20240426002

2025-3-7- 23

Home > Archive>Volume 26, Issue 2, 2025 >309-318. DOI:10.13430/j.cnki.jpgr.20240426002

Genetic Analysis of Powdery Mildew Resistance in Siyuehuang， a Wheat Landrace in Shanxi Province， China
DOI:
                        10.13430/j.cnki.jpgr.20240426002
                    
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                        NIU Huizhen1NIU Huizhen
Jinzhong Agricultural Seed Industry Breeding and Improvement Station，Jinzhong 030600，Shanxi
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REN Yongkang2REN Yongkang
College of Agricultural， Shanxi Agricultural University， Taiyuan 030031
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TANG Zhaohui2TANG Zhaohui
College of Agricultural， Shanxi Agricultural University， Taiyuan 030031
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ZHANG Xiaojun2ZHANG Xiaojun
College of Agricultural， Shanxi Agricultural University， Taiyuan 030031
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NIU Yuqi2NIU Yuqi
College of Agricultural， Shanxi Agricultural University， Taiyuan 030031
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Affiliation:1.Jinzhong Agricultural Seed Industry Breeding and Improvement Station，Jinzhong 030600，Shanxi;2.College of Agricultural， Shanxi Agricultural University， Taiyuan 030031
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Fund Project:Foundation projects： National Key R&D Program of China（2022YFD1200901）； Shanxi Province Wheat Breeding Joint Research（NYGG26）； Shanxi Agricultural University Science and Technology Innovation Enhancement Project（CXGC202305）； Breeding Engineering Project of College of Agriculture， Shanxi Agricultural University（YZ2021-0）

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Abstract:

Powdery mildew is a devastating fungal pathogen that threatens wheat production. Identifying resistant germplasm and exploring resistance genes are crucial for sustainable improvement of wheat genetic resistance. Siyuehuang， a wheat landrace identified with resistance to powdery mildew from Shanxi Province’s wheat germplasm resources， was crossed with susceptible cultivar Jinmai 47. The segregation population was developed and subjected for artificial inoculation for resistance assessment and genetic analysis at seedling stage. We employed the bulked segregant analysis （BSA） combined with a 90K SNP array to locate the resistance genes. The resistance of Siyuehuang to powdery mildew was controlled by a single dominant nuclear gene （provisionally named PmSYH）， with an inheritance pattern in accordance with Mendelian inheritance law. SNP array analysis revealed that polymorphic SNP markers were mainly enriched in the 110 Mb to 140 Mb segment on the short arm of wheat chromosome 7D. SSR （Simple Sequence Repeats） molecular markers were developed in the interval， and six markers linked to PmSYH were obtained for genotyping the F₂ population. PmSYH was mapped in the physical interval of 132.6 Mb to 137.5 Mb， flanked by Sxau7DS-37 and Sxau7DS-48 with genetic distance of 1.8 cM and 3.1 cM， respectively. Comparative analysis with previous localization results suggests that PmSYH is a new resistant gene against powdery mildew. This study provided wheat new genetic resources for wheat resistance breeding， with a certain value for improving resistance to powdery mildew in the northern winter wheat region.

Key words:common wheat;local variety;powdery mildew;resistant disease genes;molecular mapping

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